车辆超限运输对沥青路面的影响及对策

针对车辆超限运输对沥青路面的严重影响，指出在加强管理的同时，要注重超限运输车辆的检测、调查、预测和科学研究，对车辆超限运输的调查和预测要象交通量调查那样，进行统计和预测，并建立预测模型；提出沥青路面设计施工方面的措施。     

新加坡地铁公司经营管理体制及其启示

从新加坡地铁公司的经营环境出发,探讨了其经营管理体制、公司治理结构、盈利模式。在此基础上,对我国地铁公司的管理体制提出了建议。     

非正常行车机车操纵模拟系统的研究

总结机务非正常行车的主要情况,分析既有非正常行车机车操纵教学模拟系统存在的问题,提出基于SS4G电力机车模拟操纵台非正常行车操纵教学系统的设计思路和技术方案,归纳该系统的技术特点,指出该系统的改进方向。     

预制空心板结构性能检验方法探讨

预制空心板常需要进行结构性能检验，即对构件进行承载力、挠度和裂缝宽度（或抗裂）检验，本文着重介绍了其检验荷载计算和加载程序。     

公路工程施工阶段的投资管理

通过对投资的状态计算，施工过程的实时分析，评价，使施工过程的各阶段的投资消耗都控制制在计划目标内。     

航路变更后的戴直水道通航安全现状及管理对策

分析戴直水道船舶航行事故的原因，并从加强对船员的宣传、教育，增设航标，加强监控，修订《长江下游分道航行规则》以及建立VTS系统等方面提出相应对策，以消除船舶航行隐患。     

Novel experimental strategy for high resolution AFM imaging of membrane-associated bacterial toxins

Bacterial pore-forming toxins （PFTs） are essential virulence factors of many human pathogens. Knowl- edge of their structure within the membrane is critical for an understanding of their function in pathogenesis and for the development of useful therapy. Atomic force microscopy （AFM） has often been employed to structurally interrogate many membrane proteins, including PFTs, owing to its ability to produce sub-nanometer resolution images of samples under aqueous solution. However, an absolute prerequisite for AFM studies is that the samples are single-layered and closely-packed, which is frequently challenging with PFTs. Here, using the prototypical member of the cholesterol-dependent cytolysin family of PFTs, perfringolysin O （PFO）, as a test sample, we have developed a simple, highly robust method that routinely produces clean, closely-packed samples across the entire specimen surface. In this approach, we first use a small Teflon well to prepare the supported lipid bilayer, remove the sample from the well, and then directly apply the proteins to the bilayer. For reasons that are not clear, bilayer preparation in the Teflon well is essential. We anticipate that this simple method will prove widely useful for the preparation of similar samples, and thereby enable AFM imaging of the greatest range of bacterial PFTs to the highest possible resolution.     

多轮反应溶液用量对微生物加固粉土的影响

采用先将巴氏芽孢杆菌的菌液与粉土拌合,然后入渗多轮胶结液(包含等比例的氯化钙和尿素)的方法可对粉土进行有效胶结加固。反应溶液用量(胶结液入渗轮数和菌液体积)对加固效果有重要影响。胶结液入渗轮数试验结果表明：不同入渗轮数加固的试样在水中具有良好的稳定性;随着入渗轮数增加,试样中的CaCO₃含量和无侧限抗压强度逐渐提高,且入渗后期的增幅高于前期。扫描电镜结果表明：入渗轮数较多时沉积的CaCO₃晶体尺寸更大。菌液体积试验结果表明：当菌液用量为1倍孔隙体积(1.0V_v)时,试样中生成的CaCO₃相对较多,无侧限抗压强度也更高。减少菌液体积并不能带来加固效果的提升;当入渗轮数较少时,采用0.6V_v菌液体积的加固效果较差。     

船舶螺旋桨轴圆跳动的检验

简述了船舶螺旋桨轴圆跳动现象的危害,提出了实船检验的几种情况,详细介绍了实船检验的具体方法。     

路基填筑压实工法

系统地介绍了路基填筑压实工法的工艺原理、工法特点、施工工艺及施工控制等。