The exclusive economic zone: criteria and machinery for the resolution of international conflicts between different users of the EEZ

It is highly probable that one of the results of the Third United Nations Conference on the Law of the Sea will be the recognition of a new jurisdictional zone—the exclusive economic zone (EEZ). This 200-mile zone will be used for a variety of potentially conflicting purposes by both the coastal state and non-coastal states and it is therefore important to ensure that the new convention emerging from the conference should establish criteria and make provision for machinery for the resolution of the international conflicts between different users which will undoubtedly arise. This paper contains a critical analysis of the latest draft articles under consideration at the Conference and considers the criteria by reference to which disputes might be resolved.     

Tex13基因非放射性原位杂交探针的制备

目的研究制备地高辛(DIG)标记的Tex13(testis expressed gene 13)基因非放射性原位杂交探针,用于检测小鼠性腺Tex13基因的表达。方法采用RT-PCR方法扩增Tex13靶序列,将其插入带SP6和T7 RNA聚合酶启动子的pGEM-T Easy质粒,转入大肠杆菌DH10B,经蓝白斑筛选获得重组质粒,用T7和SP6引物对重组质粒进行PCR扩增,以此PCR产物为模板,以DIG RNA Labeling Mix为底物,经SP6 RNA聚合酶和T7 RNA聚合酶体外转录,分别制备DIG标记的反义链和正义链RNA探针。结果应用正常小鼠睾丸组织非放射性原位杂交实验,证实制备的探针具有较高的特异性和敏感性。结论 DIG标记Tex13基因杂交探针的成功制备,为进一步研究Tex13基因在小鼠性腺中的表达定位和发育规律以及它在减数分裂过程中的作用提供了实验基础。     

锻件材料可以延长车轮寿命

在车辆修理的总费用中,轮对维修的费用-般会占到30%.轮对维修的费用主要发生在用于车轮镟修和轮对的更换上.尽管材料因素限制了车轮的使用寿命早已成为共识,但到目前为止,在采用新型材料以显著提高车轮寿命方面并没有太大的进展.     

Prospects for maritime developments in the Baltic

Considerable change has occurred in the Baltic basin over recent years as a consequence of the re-unification of West and East Germany, political change in Polandd, the collapse of the former Soviet Union and the emergence of the independent Baltic states of Latvia, Lithuniania and Estonia. This paper reflects on the nature of the impact of these recent changes and considers trends and developments that may emerge for the future, by concentrating on the eastern Baltic regions(Poland to Russia). It is concerned more with general maritime markets and the ports and shipping interface than with the shipping industry and shipbuilding sectors of these regions. Much of the background material for the paper has been derived from participation in projects for the CIS, in Latvia and in Poland and for which the EBRD, EC (PHARE Programme) and World Bank are respectively acknowledged as the project instigator and funding agencies.     

Aspects of family and kinship in a North Carolina coastal community: a comparative study

The almost total absence of social-science data on North Carolina coastal fishing communities has provoked this preliminary study of the role played by family and kinship ties among fishermen and boat builders in such a community, with a view to developing new fisheries management strategies from an assessment of the social and economic impacts of those which already exist.     

The expansion of the national fleet of the Republic of China on Taiwan 1960-1985: the policies adopted and conflicts created

Doubting that its infant shipping industry would be able to overcome, unaided, the bottleneck it was becoming for the country's export expansion policy, the government of the Republic of China has implemented in the last 25 years a set of shipping policies to address the problem. This paper examines the details of these policies, assesses the extent to which they have succeeded, and comments on the conflicts their implementation has created within the shipping and shipbuilding sectors.