Direct resolution of the pitch of DNA on positively charged lipid bilayers by frequency-modulation AFM

High resolution structural studies of DNA and DNA binding proteins by atomic force microscopy (AFM) require well-bound samples on suitably flat substrates. Adsorbing the DNA onto a positively charged supported lipid bilayer has previously been shown to be a potentially effective strategy for structural studies with AFM. Here, using our home-built frequency-modulation AFM (FM-AFM), we show that these bilayer substrates are only maximally effective for high resolution AFM when the samples are short, linear DNA, compared with circular plasmid DNA. We find that, with the former sample, the measured width of the DNA is about 2 nm, the known DNA diameter, and there is a clear height modulation along the length of the DNA with a periodicity of about 3.4 nm, in excellent agreement with the known pitch of the double helix. This sample preparation strategy is expected to enable higher resolution studies of DNA and DNA binding proteins with FM-AFM than that can presently be achieved.     

The case for space: the measurement of capacity utilisation,its relationship with reactionary delay and the calculation of the capacity charge for the British rail network

In Britain, capacity charges are levied on train operators to cover the costs imposed by increased reactionary delays – i.e. the delay that an already late train causes to a following train. These charges are based on the link between capacity utilisation and the level of reactionary delay. An established method for measuring capacity utilisation was adopted called The Capacity Utilisation Index (CUI). In this paper, we propose an alternative methodology based on the theory that the level of reactionary delay will be determined by the minimum gaps that exist between trains. We test this measure with a new data-set for the East Coast Main Line and show that it performs better than CUI. Finally, we comment on the implications of this finding for charging and for the construction of timetables.     

Wave propagation in head on bonnet impact: Material and design issues

Head on bonnet impact is becoming more and more important in automotive design as regulations on pedestrian safety become more demanding. Despite the relatively low amount of energy involved, these impacts are truly dynamic phenomena as the event duration is comparable with the traveling time of the different wavefronts generated by the impact. In this paper, we show that we can build up a simplified model for the impact based on wave propagation analysis. Using this model, we can analyze head acceleration on existing bonnets or predict it on new ones. Head acceleration in a bonnet impact can thus be estimated over the whole area of the bonnet with a few minutes of CPU.     

Transportation planning for special events: a review of expo 86 in Vancouver

The authors examine the problems of transportation planning for special events such as Worlds Fairs, Olympic Games etc., and use as a case study the experiences gained at EXPO 86 in Vancouver. Results indicate that sufficient flexibility exists in the urban infrastructure to accommodate the transport access and parking requirements of major, short‐term special events.     

Causal linkages between highways and sector-level employment

While transport infrastructure investments have usually been viewed to have long-term impacts on employment, what is perhaps not immediately clear is the direction of causality. This paper has sought to disentangle the causal relationship between highway infrastructure and employment, using panel data for the 48 contiguous US states from 1984 to 1997. Of particular emphasis in this analysis is the sectoral differences in the causal and spatial effects of highway capacity expansions for employment growth in alternative sectors of the economy. The results indicate that lane-mile additions of own-state major highways could increase state employment growth in the services sector while reducing growth in manufacturing. However, the causal relationship is also found to work the other way around. That is, both the rapid growth in services employment and the slowdown in manufacturing jobs temporally lead to increases in roadway capacity of non-interstate major roads. Our analysis also shows that highway infrastructure could produce both positive and negative employment spillovers across states. We find that improvements in non-interstate major roads outside the state border are beneficial to the manufacturing sector which generally serves regional and national markets. For the services sector, however, employment gains from interstate highways in the same state may come at the expense of other states as there is clear evidence of negative employment spillovers from interstate lane-mile additions.     

Diatom stratigraphy and long-term dissolved silica concentrations in the Baltic Sea

In many parts of the world coastal waters with anthropogenic eutrophication have experienced a gradual depletion of dissolved silica (DSi) stocks. This could put pressure on spring bloom diatom populations, e.g. by limiting the intensity of blooms or by causing shifts in species composition. In addition, eutrophication driven enhanced diatom growth is responsible for the redistribution of DSi from the water phase to the sediments, and changes in the growth conditions may be reflected in the sediment diatom stratigraphy.To test for changes in diatom communities we have analyzed four sediment cores from the Baltic Sea covering approximately the last 100 years. The sediment cores originate from the western Gulf of Finland, the Kattegat, the Baltic Proper and the Gulf of Riga. Three out of the four cores reveal only minor changes in composition of diatom assemblages, while the Gulf of Riga core contains major changes, occurring after the second World War. This area is set apart from the other Baltic Sea basins by a high frequency of low after spring bloom DSi concentrations (< 2 µmol L^− 1) during a relatively well defined time period from 1991–1998. In 1991 to 1993 a rapid decline of DSi spring concentrations and winter stocks (down to 5 µmol L^− 1) in the Gulf was preceded by exceptionally intense diatom spring blooms dominated by the heavily silicified species Thalassiosira baltica (1991–1992; up to 5.5 mg ww L^− 1). T. baltica has been the principal spring bloom diatom in the Gulf of Riga since records began in 1975. DSi consumption and biomass yield experiments with cultured T. baltica suggest that intense blooms can potentially exhaust the DSi stock of the water column and exceed the annual Si dissolution in the Gulf of Riga. The phytoplankton time series reveals another exceptional T. baltica bloom period in 1981–1983 (up to 8 mg L^− 1), which, however, took place before the regular DSi measurements. These periods may be reflected in the conspicuous accumulation of T. baltica frustules in the sediment core corresponding to ca. 1975–1985.     

Bridging the gap between complex data and decision-makers: an example of an innovative interactive tool

Abstract

The increasing capacity of technological tools, as well as the advent of geographic information systems, has multiplied the ability to process large sets of microdata. At the same time, modeling concerns and the quest for a more in-depth understanding of individual behaviors is requiring more, and better, data. The gap between scientific modeling and informational tools for decision-making seems to be widening.

In the Greater Montreal area, large sets of microdata have been available for more than 30 years. Along with the development of modeling and planning tools, particular attention has been given to the continuous enlightening of planners and decision-makers with respect to the outcomes of the various surveys. This has led to the development of particular interactive tools, specifically addressed at local planners, which present the most significant information regarding travel and demography, for geopolitically relevant areas.

This paper presents the most recent interactive tool that has been developed for local planners in the Montreal area. It integrates information from both the most recent travel survey held in the region and the Canadian census. It offers both ease of use and relevant analytical means to assist in exploring the complex relations between spatial locations, demographic features, and activity-travel indicators.     

MINOE: A Software Tool to Analyze Ocean Management Efforts in the Context of Ecosystems

Transitioning ocean governance into an integrated ecosystem-based approach requires improved knowledge of existing governance arrangements. This article presents a software tool, MINOE, to assist policymakers, scientists, and others involved in ecosystem-based management initiatives to navigate through management documents as they relate to a user-defined ecosystem. The tool uses a conceptually modeled ecosystem, defined by the user, and text analysis of a set of management-related documents to determine which ecosystem linkages are potentially acknowledged in the documents. For illustration, the set of documents included with MINOE currently (and used to demonstrate the software in this article) are laws and regulations from four geopolitical jurisdictions for the year 2006; however, users may also import other documents for a more tailored application. Features include an interactive matrix containing results about the set of management documents within the user's scope and scale of interest. In addition, MINOE includes metrics and visualization tools to synthesize information derived from the documents. The article presents the software tool, describes potential uses for the tool, and ends with a discussion of future work to expand the program.     

Have tropical marine protected areas worked? An initial analysis of their success

A multinomial choice framework was used to analyze data from hypothetical storm forecast scenarios administered via mail survey to a random sample of U.S. Gulf Coast residents. Results indicate that the issuance of a mandatory evacuation notice and the presence of higher wind speeds had the largest influence on increasing the likelihood of evacuation. Age, race, disability, distance, and education were significant in explaining one's decision to wait relative to choosing to evacuate. Blacks and disabled individuals were strictly less likely to wait and more likely to make an immediate evacuation decision. Hurricane Katrina evacuees and those with an evacuation destination identified were also more likely to decide to evacuate, but were also more likely to wait before deciding. Results indicate that residents of mobile homes were more likely to either evacuate or wait before making a decision, but strictly less likely not to evacuate. Respondents very confident in being rescued were strictly more likely not to evacuate. Results indicate that not having an evacuation destination identified was the most influential factor regarding the likelihood of not knowing what choice to make.     

The visual effects of port redevelopment alternatives

Abstract

In the early 1970s, Seattle Piers 90/91 were declared surplus federal property by the GSA. These obsolescent half‐mile‐long piers and their adjacent uplands were purchased by the Port of Seattle for redevelopment. This urban waterfront site of almost 200 acres presents the Port of Seattle with a number of severe planning problems. While it is the last large deep‐water site on Elliott Bay, it is also in a very sensitive location, since it is overlooked on two sides by vocal residential communities with a history of active intervention in development projects on visual and other environmental grounds.

At Piers 90/91, public concerns over the visual effects of port redevelopment include:

1. nuisance effects of night‐operations lighting on adjacent hillside residences;

2. nighttime obstruction of distant views due to increased foreground illumination;

3. day and night view obstruction by tall structures such as container‐handling cranes;

4. alteration of the character of both day and nighttime views by new port facilities.

As part of a program to study the effects of alternative redevelopment strategies for Piers 90/91, the Port has undertaken a through examination of the visual effects of different uses, their visual appropriateness, and the ways by which adverse visual effects can be mitigated. The results were incorporated into an environmental impact statement which documented compliance with the Seattle Shoreline Master Program and with view protection and glare provisions of the City's S.E.P.A. guidelines.

A systematic Visual Resource Management (VRM) approach was successfully employed on this coastal zone project. Major elements included visibility mapping, key view selection, analysis of existing visual character, simulation of alternatives, assessment of relative visual compatibility, and determination of effective mitigation measures. Community involvement has also been a critical element in this approach to assessing and managing the visual effects of redevelopment in a major urban port.