PROPERTIES OF PROLIFERATION AND DIFFERENTIATION OF NEONATAL RAT RETINAL PROGENITOR CELLS IN VITRO

Objective To investigate the properties of proliferation and differentiation of neonatal rat retinal progenitor cells （RPCs） in vitro. Methods RPCs were isolated from neonatal SD rats neural retina and cultured in DMEM/F12 ＋ N2 with EGF and bFGF （suspension medium ） or 10 % FBS without EGF and bFGF （ differentiation medium）. The cells grew as suspended spheres or adherent monolayers, depending on different culture conditions. The neural stem cells or retinal progenitors, neurons, astrocytes, retinal ganglion cells, rod photoreceptors and the proliferating cells were evaluated with immunofluorescence analysis by Nestin or Pax6, Map2, GFAP, Thy-1, Rhodopsin and BrdU antibodies respectively. Results RPCs could propagate and differentiate in suspension or differentiation medium and express the markers of Nestin （92.86%） or Pax6 （86.75%）, Map2 （38.54%）, GFAP （20.93%）, Thy-1 （27. 66%） and Rhodopsin（13. 33%）in suspension medium; however, Nestin （60.27%）, Pax6 （52%）, Map2 （34.94%）, GFAP （38.17%）, Thy-1 （30.84%） and Rhodopsin （34.67%） in differentiation medium. 96.4 % of the population in the neurospheres was BrdU-positive cells. The cells could spontaneously adherent forming some subspheres and retinal specific cell types. Conclusion Neonatal rat RPCs possess the high degree of proliferation and can differentiate into neurons, astrocytes, retinal ganglion cells and rod photoreceptors in vitro. There are different proportions for RPCs to differentiate into specific cell types.     

燃料电池轿车动力系统结构设计

对比分析实际燃料电池轿车两种动力系统结构的优缺点,提出燃料电池轿车动力系统设计原则;Plug-in燃料电池轿车动力系统的结构能够继承所述两种动力系统结构的优点并克服其固有缺点;根据动力系统设计原则实现的Plug-in燃料电池轿车动力系统,具有效率高、成本适中的特点;经仿真分析和试验认证,所设计的Plug-in燃料电池轿车动力系统结构和能量分配设计方法准确实用。该方法对串联混合动力、双电源系统汽车的能量设计分配也具一定参考价值。     

钢桥面环氧沥青防水粘结层耐久性影响分析

大量现场调查表明,因防水粘结层失效而造成的钢桥面铺装层脱层或滑移是造成钢桥面铺装病害的重要原因之一,交通荷载、温度、水等因素通常被认为是影响铺装防水粘结层长期使用的主要因素。根据钢桥面铺装特点与技术要求,选择环氧沥青作为防水粘结层进行试验研究。考虑两种温度(25℃、70℃)的拉拔试验和剪切试验,明确了防水粘结层的温度敏感性;选择不同表面构造钢板进行拉拔试验,考察其对环氧沥青防水粘结层粘结性能的影响;以高温浸水拉拔试验和剪切试验,评价了高温水对钢桥面铺装防水粘结层的影响;试验结果表明:随着温度的升高,防水粘结层的拉拔强度和抗剪强度下降很快;随着浸水时间的增加,拉拔和抗剪强度下降较慢,且抗剪强度基本不变。因此,采用环氧沥青作为钢桥面防水粘结层时,温度应作为首要考虑因素。     

Correlation of expression of ER, PR and c-erbB-2 with ultrasonographic features in invasive ductal cancer of breast

Objective To analyze the relationship of the expression level of estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor-2 (HER-2, c-erbB-2) of breast invasive ductal carcinoma (IDC) with ultrasonographic characteristics. Methods Totally 104 patients with IDCs confirmed pathologically were involved in this study. ER, PR and c-erbB-2 expression in the IDC specimens was determined by immunohistochemical staining technique. The correlation between the ultrasonographic features and the positive expression of ER, PR or c-erbB-2 was analyzed. Results The positive expression rate of ER and PR in the group with tumor spiculation sign and posterior acoustic attenuation was higher than that in the group without. The positive expression rate of ER differed significantly (P<0.05) while that of PR did not (P>0.05). The over-expression rate of c-erbB-2 in the group of microcalcification, sufficient blood flow and axillary lymph node metastasis was higher than that in the group of non-microcalcification, deficient blood flow or without axillary lymph node metastasis (P<0.05). The expression of ER, PR and c-erbB-2 was not related to the size of tumor (P>0.05). Conclusion The expression of ER and c-erbB-2 is closely related to the ultrasonographic characteristics of IDC, which may, to some extent, reflect the expression level of ER and c-erbB-2.     

THE ANALYSIS OF HUMAN CYTOMEGALOVIRUS INFECTION DURING PREGNANCY IN QINBA MOUNTAINOUS AREA

Humancytomegalovirus (HCMV )isoneofthemostcommoncongenitalvirusinfectioninhu manandalsousuallythecauseofmentalretardationandhearinglossofchildreninthedevelopedworld .TheactiveinfectionpercentageofHCMVisabout0 .3% - 12 .0 %there ,butwithregardtothedevel opingcountries ,especiallythepovertymountainousareas,the percentageofCMVinfectionisun known .QinbamountainousareainShaanxiisapoverty strikingarea ,wheretheeconomicconditionandtheculturearebackwardandtheincidenceofMRishigh .TostudyCMVinfec…     

基于零部件VOC散发的材料及工艺研究

为了解某品牌车型近年来内饰件VOC现状,利用袋子法对该品牌所有车型的内饰件进行了分析,测试和统计结果表明零部件VOC中甲苯、二甲苯、甲醛、乙醛存在控制风险;对座椅总成和仪表板总成2个重点零件的材料法测试结果进一步分析,发现泡沫、面料和PP类塑料件等是VOC的主要来源。最后,对不合格原因进行简要分析,并从原材料、工艺、仓储、装备等方面给出相应的优化方案。     

国产轻型汽车排放现状与分析

本文综述了国产轻型汽车的基本情况，指出轻型汽车的排气污染物是造成汽车对大气污染的最主要来源，根据试验结果评述了国产轻型汽车的排放现状，然后进行了试验分析，最后简要介绍了影响汽车对大气污染的各种因素。     

高速铁路轨道基准网测量与数据处理新方法

高速铁路轨道基准网在我国工程测量领域是个新生事物,最近几年才从德国引进。本文在学习德国测量技术的前提下,结合这几年在我国的应用情况,在分析总结其优缺点的基础上,提出与德国方法完全不同的、具有自主知识产权的CPⅣ网测量与数据处理的新方法。新方法中采用13m间隔布设CPⅣ点,CPⅣ点采用强制对中标志,采用构网法进行CPⅣ平面网的自动测量,根据中视法的高差构建具有多余观测的CPⅣ高程网,对CPⅣ平面网和CPⅣ高程网进行严密间接平差和验后精度评定。新的CPⅣ网测量精度和测量效率更高,数量处理理论更严谨。观测实验结果表明：本文提出的新方法理论严谨、切实可行,值得推广应用。     

The β-amyloid protein induces S100β expression in rat hippocampus through a mechanism that involves IL-1

Objective To explore the effect of β-amyloid protein (Aβ) on S100β expression in rat hippocampus and its mechanisms. Methods At 7 days after bilateral stereotaxis injection of different dose of fibrillar Aβ 25-35 and interluekin-1 receptor antagonist (IL-1ra) into the rat CA1 region, the learning and memory abilities of rats were tested with passive avoidance task. Amyloid deposition was detected by using Congo red staining technique. Nissl staining and immunohistochemical techniques were used to analyze the number of neurons, and GFAP and the S100β expression in hippocampal CA1 region , respectively. Results After fibrillar Aβ injection, the step-through latency of rats was significantly shortened compared to that of the control group. The GFAP positive astrocytes were found surrounding amyloid deposition. Neuronal loss occurred in the pyramidal cell layer of CA1 region. The number of S100β positive cells in Aβ-treated group was significantly increased compared with that in the control group. After IL-1ra injection, the number of S100β positive cells was significantly decreased. Conclusion Intrahippocampal injection of Aβ 25-35 could cause similar pathologic changes of Alzheimer's disease. Aβ 25-35 was capable of up-regulating S100β expression in a dose-dependent manner. The injection of IL-1ra could attenuate the effect of Aβ on S100β expression.     

PROLIFERATION AND MIGRATION OF EPENDYMAL CELLS IN THE BRAIN OF ADULT RATS AFTER PERMANENT FOCAL CEREBRAL ISCHEMIA

Objective Ependymal cells are thought to be the primary source of neural stem cells in the adult central nervous system. The purpose of this study is to examine spatial and temporal profiles of ependymal cell proliferation and migration after focal cerebral ischemia. Methods Eighty male Sprague Dawley rats underwent permanent middle cerebral artery occlusion after injection of 10/μL of 0.2% Dil into the lateral ventricle. Rats were sacrificed and brain sections were acquired for pathological evaluation and laser confocal imaging at day 1,3,7,11,14,21 and 28 after ischemia. Results The density of Dil-labeled cells in the ischemic ipsilateral subventricular zone was significantly higher than that in the control group and these labeled cells dispersed in the ischemic ipsilateral subventricular zone and/or were located in ependyma from day 1 to 11. In the ischemic ipsilateral cortex, some Diilabeled cells occurred in peri-infarction and infarction of parietal region at day14 and peaked at day 21 when some Dil-labeled cell nodules were found in this region. During postischemic day 14--28, a significant decrease in labeled celldensity in the ischemic ipsilateral subventricular zone was coincident with a significant increase in labeled cells density in the cortex (peri-infarction and infarction). Conclusion The results indicate that ependymal cells proliferate and migrate after focal cerebral ischemia in the adult rat brain.