钢箱梁桥地震响应的有限元分析

针对典型的工程实例,基于大型有限元软件,采用纤维模型模拟梁柱单元,建立了钢箱梁桥的三维有限元模型,合理选取了强震记录,进行了罕遇地震作用下桥梁结构的弹塑性时程分析,为桥梁结构抗震设计和提高抗震安全性提供了科学依据和重要参考.     

桥梁加宽中新增梁对旧桥的影响

依托黄土地区高速公路改扩建桥梁加宽工程,选取一座3孔13 m简支空心板桥,分析新增空心板梁对旧桥原有板梁的影响。基于不同加宽方案对新旧桥空心板梁横向分布系数进行了计算,计算结果表明增加新梁截面刚度可有效降低旧梁横向分布系数。建议采用移旧桥边梁为新桥边梁并且提高新增空心板梁刚度的加宽方案。     

四川旅游资源交通系统综合布局分析

作为影响旅游活动的重要因素之一,交通布局直接关系到旅游业的发展。本文根据四川旅游资源的现状、地理位置等情况,将四川划分出三大旅游经济圈,从各旅游经济圈交通现状及客流特征入手,利用关联分析的方法,结合数据对比,剖析了各旅游经济圈与交通的关联。最后,在整合四川旅游资源的前提下,得出了四川旅游资源交通系统综合布局的相关结论。     

区域道路运输相对效率截面分析

随着区域路网的完善,其承载的道路运输业也呈现快速发展态势。在区域层面上,实现两者的良性互动,需要因地制宜,实现区域与运输业阶段性的协调决策,进而保证在全社会范围内对优化资源配置。这些分析的实现需要合适的评价方法对道路运输业投入要素与产出要素间的内在关系进行深入研究,并能对投入、产出要素进行量化控制。本文引入数据包络分析方法（Data Envelopment Analyses,简称DEA方法）,采用CCR模型（Charnes,Cooper and Rhodes Model）以各区域道路运输为决策单元;构筑各区域道路运输生产前沿面,评价各单元的相对效率,分析各要素投入的变动对产出的影响。     

大型活动交通影响评价方法

大型活动吸引和产生的突增交通量会造成周边地区的交通拥挤。通过比较大型活动与大型商业建设项目的交通影响评价特点和分析大型活动交通管理的影响因素,分别针对“提供一次性服务的大型活动”和“提供长效服务的大型活动”,设计了交通需求的预测方法。以交叉口服务水平分析方法为基础,提出一种了大型活动的交通影响评价方法。以长春市南岭体育场举办的“中国足球甲A联赛”为对象,运用Vissim微观交通仿真软件对所提出的方法进行了初步验证。结果表明,在大型活动条件下,所提出的方法能够确定需要进行交通管制的空间范围。     

Modeling research on manufacturing execution system based on large-scale system cybernetics

A cybernetics model of manufacturing execution system(MES CM) was proposed and studied from the viewpoint of cybernetics.Combining with the features of manufacturing system, the MES CM was modeled by"generalized modeling"method that is discussed in large-scale system theory.The mathematical model of MES CM was constructed by the generalized operator model, and the main characteristics of MES CM were analyzed.     

Effects of rosiglitazone on rabbit atherosclerosis

We sought to validate magnetic resonance imaging(MRI) for documenting the effects of rosiglitazone on rabbit atherosclerosis and the possible mechanism by treatment on the lesions.24 New Zealand white rabbits were randomly divided into normal group, control group and treatment group.After 4 weeks, all rabbits underwent MRI.After examination, the number of circulating endothelial progenitor cells(EPCs) and level of lipid, glucose, nitric oxide(NO) and superoxide anion(O-2·) were measured.MRI demonstrated the regression of atherosclerotic lesions by rosiglitazone which has good correlation with histopathology.Treatment not only increased the level of EPCs and NO, but also raised the ability of inhibition of O2-·.MRI is a promising noninvasive technology to detect the effects of therapeutic interventions.Rosiglitazone slows the progression of atherosclerosis that may in part attribute to its improvement of mobilization of EPCs and the reduced oxidative stress.     

Sensitivity analysis of parameters for multi-stand roll forming using a new booting model

Multi-stand roll forming is a process that has very complicated deformation behaviour and shows significant nonlinearity.In this paper, the sensitivity analysis of parameters for multi-stand roll forming was performed via a new booting finite element method(FEM) model.Compared with the most of simulation, the new model is more consistent with production process and can account for the effects of roll rotating speed.Based on the model, the process of an open section channel formed with 10 passes was simulated and the sensitivity analysis was conducted with orthogonal experiment design combined FEM model.The multi-stand roll forming process can be efficiently analyzed by the new booting model.And sensitivity analysis shows the hardening exponent plays an important role in controlling the quality of the products.     

幽门螺杆菌尿素通道蛋白基因UreI载体的构建、融合表达及纯化

目的构建幽门螺杆菌(H.pylori,Hp)尿素通道蛋白基因UreI的融合表达载体,并在E.coliBL21中表达,为进一步研究UreI的功能奠定基础。方法利用分子克隆技术以Hp DNA染色体为模板,扩增Hp UreI基因片段,将目的基因UreI与载体pET32a( )分别经kpnⅠ和HindⅢ双酶切,纯化,连接。筛选阳性重组载体,转化大肠杆菌BL21,以IPTG诱导表达pET32a( )/UreI融合蛋白。表达产物经Ni-NTA琼脂糖树脂纯化后,用SDS-PAGE及Western blot分析。结果经酶切、测序分析表明,插入的基因片段为Hp UreI编码基因,由585 bp组成,与GenBank相应菌株HP AM417 609序列完全一致,无碱基突变。经SDS-PAGE分析显示重组质粒pET32a( )/UreI在原核细胞中得到了高效融合表达,其重组融合蛋白分子量为130 ku,经Ni-NTA琼脂糖树脂纯化后,其纯度达95%以上,Western blot分析显示,该重组融合蛋白可被6-His鼠抗单克隆抗体所识别,具有良好的反应原性。结论成功地克隆和构建了原核表达载体pET32a( )/UreI,并在原核细胞中得到了高效融合表达。     

掺锶磷酸钙骨水泥的体外细胞生物学性能评价及其与掺锶量的相关性

目的 评价"SrHPO4/Ca4(PO4)2O/CaHPO4"系掺锶磷灰石骨水泥的生物相容性及其与掺锶量的关系.方法 通过急性全身毒性、热原性、溶血性、体外细胞毒性以及成骨细胞在材料表面的黏附、增殖与表达等实验,检测材料的性能及生物降解潜能.结果 "SrHPO4/Ca4(PO4)2O/CaHPO4"系掺锶磷灰石骨水泥的急性全身毒性、热原性、溶血性、体外细胞毒性均为合格.Sr-CPC的热原性、溶血性以及碱性磷酸酶值与掺锶量呈非线性关系.结论 含锶磷酸钙骨水泥体外细胞生物相容性良好,是安全的新型骨组织工程支架材料.