THE EXPRESSION OF BCL-2, BAX AND CASPASE-3 IN NEURON OF THE SPINAL CORD ANTERIOR HORN AFTER CAUDA EQUINA ACUTE COMPRESSION

Objective To explore the influence of the acute cauda equina compression on the iumbosacral spinal cord; To clarify the pathologic change of the motor neuron after acute cauda equina compression. Methods 27 canis familiaris were randomly divided into 9 groups （3 in each） ： one for normal group, one for control group, and seven for compression groups. The control group and compressed groups was given operation and the sac made of silica gel was placed under the lamina of L5-6. Water was injected into the sac until their posterior legs paralysis in compressed groups, the animals had been compressed for 4, 8, 12, 24, 48, 72, 168 hours. The control group were not injected water. Cells apoptosis was investigated with the technology of TdT-mediated biotin dUTP nick end-labeling （TUNEL） staining. The Bcl-2 Bax and Caspase-3 protein was investigated by immunohistochemical method. Results TUNEL staining cells in anterior horn presented after compressed 8-12 hours, and at 72 hours the number of positive cells got to maximum, it decreased subsequently after 168 hours. The protein of Bax, Bcl-2 expressed a little in normal motor neuron. The caspase-3 protein didn＇t express in normal ceil. They all reached the peak at 72 hours after compression. Conclusion The apoptosis of motor neuron occurred earlier after eauda equina acute compression. Bax protein restrained Bcl-2 protein then active caspase-3 and conduced apoptosis of motor neuron.     

脾虚证大鼠侧脑室注射孤啡肽对细胞免疫功能的影响

目的脾虚证大鼠侧脑室注射孤啡肽,研究其对细胞免疫功能的影响,从神经内分泌的角度探索脾虚的细胞免疫学变化本质。方法用100%大黄煎剂灌胃复制脾虚证大鼠模型,四君子汤作为反证药物,侧脑室埋管,注射孤啡肽,MTT法检测脾T淋巴细胞增殖能力、足跖增厚法检测迟发型变态反应程度,判定细胞免疫功能改变情况。结果灌服大黄煎剂后,大鼠逐渐出现腹泻,或伴有脱肛,食量减少,精神不振,懒动,被毛失去光泽,形体消瘦等脾虚症状,而空白对照组(BC组)没有上述症状;T淋巴细胞增殖能力、迟发型变态反应程度与BC组比较明显减弱(P<0.01)。四君子汤治疗组(DFMD组)和孤啡肽组(OFQ组)大鼠上述脾虚症状及检测指标明显改善(P<0.01)。结论脾虚证大鼠侧脑室注射孤啡肽能提高细胞免疫功能。     

BPON系统的ONT研究和嵌入式系统设计

宽带无源光网络（BPON）系统是一种优良的高速、宽带接入解决方案,是实现光纤到户（FTTP）的重要解决方案之一.在介绍BPON系统的网络分层结构基础上,分析了光网络终端（ONT）设备实现的主要功能,讨论了实现方案.利用嵌入式思想开发ONT系统,并研究了软件设计的主要方法.将软件划分为5个模块,设计出每一模块的流程,并讨论了其中的消息处理核心功能的实现过程.     

Catalytic Combustion of Lean Methane Assisted by Electric Field over Pd/Co<Subscript>3</Subscript>O<Subscript>4</Subscript> Catalysts at Low Temperature

A series of Pd/Co₃O₄ catalysts were prepared by Self-Propagating High-Temperature Synthesis (SHS) method in this study, and electric field was applied for catalytic combustion of lean methane over Pd/Co₃O₄ catalysts at low temperature. When electric field was applied, the catalytic combustion performance of Pd/Co₃O₄ catalysts was greatly improved, and the application of electric field could reduce the load of active element Pd to some extent while maintaining the same efficiency. Based on experimental tests and the analysis results of X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), H₂-temperature-programmed reduction (H₂-TPR) and in-situ diffuse reflectance infrared Fourier transform spectroscopy (in-situ DRIFTS), the mechanism of catalytic oxidation of CH₄ over Pd/Co₃O₄ catalysts in electric field was proposed. The catalytic combustion of CH₄ occurs only when the temperature is higher than 250 °C normally, but when electric field was applied, the whole process of CH₄ oxidation was promoted significantly and the reaction temperature was reduced. Electric field could promote the reduction of the support Co₃O₄ to release the lattice oxygen, resulting in the increase of PdO_x and the surface chemisorbed oxygen, which could provide more active sites for the low-temperature oxidation of CH₄. Furthermore, electric field could accelerate the dehydroxylation of CoOOH to further enhance the activity of the catalysts.     

对海员培训收费标准的探讨

STCW78/95公约已于1997年2月1日生效,公约要求各缔约国最迟应于1998年8月1日实施STCW78/95公约.对于已于1998年8月1日前进入海员队伍的人员,以及在接受教育和培训的人员,最迟在2002年2月1日前满足STCW78/95公约要求.我国是该公约的缔约国,海员的各项履约培训是当前航海教育的迫切任务.广泛开展的海员履约培训全面提高了我国海员的技术素质,使我国海员队伍符合国际标准,为保证我国海运事业发展以及发展海员劳务输出作出了贡献.各院校和培训单位在从事履约培训中提高了办学水平也获得了一定经济效益.我国海员培训正在进一步发展中.但是几十年来旧的计划经济的教育模式是:"办学是完成上级交给的任务,投入是国家拿钱".旧观念严重制约了海员培训教育的发展,培训收费不合理,收费与投入不成比例,使培训单位不能自我积累自我发展.在国家不能投入更多资金发展航海教育的情况下,航海教育培训投入不足,设备短缺,培训仪器落后,使航海教育培训远跟不上日趋现代化的航运事业发展的需要,制约了航海教育培训进一步发展提高.     

隧道坡度因素对柴油车烟雾排放量的影响

通过发动机台架模拟 ,控制吸入气缸一定废气量而营造隧道污染环境 ,进行柴油车在实际道路坡度运行时动态烟雾排放测试 ,以及在实际隧道环境下 ,柴油车坡度运行时动态烟雾排放测试 ,获得在不同坡度环境下柴油车烟雾排放的道路实测数据。结果分析表明 ,随着道路坡度的增加 ,柴油车烟雾排放量急剧上升 ,而在相同坡度下 ,车速越高烟雾排放量越大 ,且隧道内坡度每增加1 % ,柴油车烟雾排放量大约增加 70 %～ 1 2 0 %。     

水飞蓟宾对人膀胱癌细胞株5637增殖的抑制作用及其机制

目的研究水飞蓟宾(Silibinin,SB)在体外对人膀胱癌细胞株5637增殖的抑制作用及其相关机制。方法采用MTT法观察不同浓度SB对人膀胱癌细胞株5637增殖的抑制作用;倒置显微镜下观察细胞形态变化;RT-PCR检测凋亡抑制因子Survivin的mRNA表达变化。结果MTT检测发现:随着SB浓度增加和作用时间延长,对人膀胱癌细胞株5637增殖的抑制作用增强,且各组间比较均有显著性差异(P<0.01),并呈时间-剂量依赖关系;倒置显微镜发现应用SB干预后,5637细胞体积缩小,变形,胞浆浓缩,核固缩深染;人膀胱癌细胞株5637中Survivin的mRNA表达随着SB浓度增加和时间延长相应下降。结论SB可抑制人膀胱癌细胞株5637的增殖;并下调凋亡抑制因子Survivin的mRNA表达。     

硒对培养人肝细胞的毒性作用

原代培养人胚肝细胞，观察外加不同剂量亚硒酸钠对肝细胞生长代谢的影响。结果表明，当培养液中硒的作用浓度增至1．156×10－6mol／L时，肝细胞聚缩、脱落，丙氨酸转氨酶外释增多，白蛋白合成减少，存活率下降。提示过量硒具有明显的肝细胞毒性作用。     

铁路枢纽作业轮廓计划优化模型及算法

本文给出铁路枢纽日常运输组织工作轮廓计划多商品时空网络流模型及算法，对次日枢纽内大小车流、机车应用配合我纽内各站装、卸、排空计划作出合理安排。揭示枢纽内各站次日工作的重点与关键。为进一步编制日班计划及阶段计划提供指导及辅助决策基础。