Novel experimental strategy for high resolution AFM imaging of membrane-associated bacterial toxins

Bacterial pore-forming toxins （PFTs） are essential virulence factors of many human pathogens. Knowl- edge of their structure within the membrane is critical for an understanding of their function in pathogenesis and for the development of useful therapy. Atomic force microscopy （AFM） has often been employed to structurally interrogate many membrane proteins, including PFTs, owing to its ability to produce sub-nanometer resolution images of samples under aqueous solution. However, an absolute prerequisite for AFM studies is that the samples are single-layered and closely-packed, which is frequently challenging with PFTs. Here, using the prototypical member of the cholesterol-dependent cytolysin family of PFTs, perfringolysin O （PFO）, as a test sample, we have developed a simple, highly robust method that routinely produces clean, closely-packed samples across the entire specimen surface. In this approach, we first use a small Teflon well to prepare the supported lipid bilayer, remove the sample from the well, and then directly apply the proteins to the bilayer. For reasons that are not clear, bilayer preparation in the Teflon well is essential. We anticipate that this simple method will prove widely useful for the preparation of similar samples, and thereby enable AFM imaging of the greatest range of bacterial PFTs to the highest possible resolution.     

进气掺氢与富氧燃烧对汽油机性能影响的试验研究

在JL3G10汽油机的基础上,搭建了发动机台架以进行掺氢富氧条件下的台架试验。利用该台架分别对不同进气含氧量(体积比),不同进气掺氢比以及富氧掺氢时汽油机的动力性与排放进行了试验研究。研究表明:相比原机,进气含氧量为25%时汽油机功率与扭矩提高了20.7%,HC排放减少36%,CO排放减少10.6%,但NOx排放增加了149.6%;2%进气掺氢比下的HC排放相比原机降低31.2%,CO排放降低46.1%,NOx排放则增加12.6%;富氧掺氢(氢氧体积比为2∶1)时,掺混比例为5.06%的汽油机较原机在动力性与排放上均有提升。     

客运专线抗台风措施研究

客运专线高速车辆轻量化,使其抗强风能力减弱。为保证客运专线安全运输,从线路、路基、桥梁、隧道、站场及电气化设施等方面提出抗台风的设计原则,可采用的运输安全监控预警及运营安全措施。以上研究成果可确保沿海铁路的运营安全,进一步提高沿海铁路建设质量。     

Identity-based broadcast encryption with shorter transmissions

This paper describes two identity-based broadcast encryption(IBBE) schemes for mobile ad hoc networks.The first scheme proposed achieves sub-linear size cipertexts and the second scheme achieves O(1)-size ciphertexts.Furthermore, when the public keys are transmitted, the two schemes have short transmissions and achieve O(1) user storage cost, which are important for a mobile ad hoc network.Finally, the proposed schemes are provable security under the decision generalized bilinear Di?-Hellman(GBDH) assumption in the random oracles model.     

TNFα、Leptin-Rb、NF-κB和PPAR-γ在酒精性肝病大鼠肝细胞中的表达

目的 探讨TNFα、NF-κB、Leptin-Rb和PPAR-γ等在大鼠酒精性肝病中与肝组织炎症、坏死和纤维化的关系.方法 胃造瘘法建立长期大鼠酒精性肝病模型,分别在第4、8、12、24和52周用免疫组化、原位杂交和RT-PCR技术检测不同阶段大鼠肝组织中TNFα、NF-κB、PPAR-γ和Leptin-Rb等的表达.结果 TNFα、NF-κB和Leptin-Rb水平在模型组大鼠肝细胞中,第8周表达逐渐增强,第24周起高水平表达一直持续至52周(P<0.01).PPAR-γ在对照组和第4、8周模型组肝细胞中表达最强,第12周表达开始减弱.TNFα和NF-κB在肝细胞中的表达呈明显正相关关系,与PPAR-γ则呈负相关关系(P<0.01).结论 TNFα和NF-κB与肝细胞病变程度有很好的一致性关系,Leptin-Rb在肝细胞的表达与肝纤维化发展相一致,PPAR-γ则刚好相反.     

某商用车散热器面罩开裂分析与改进

为解决散热器面罩开裂问题,提出了焊点优化方案和散热器面罩加强板外置/加强板内置方案,并进行了模块化设计,制定了2组改进措施。有限元分析结果表明:2种改进措施与原状态相比,均有良好应力改善效果,满足应力要求。     

Geoswath Plus多波束水深数据抽稀算法研究

根据多波束数据量大的特点,采用VC++程序开发语言,结合工程实际需要,开发出一套多波束数据抽稀程序,有效解决了用户从原始多波束水深数据中提取所需数据的问题。     

基于PLC与变频器的船用载货电梯控制系统研究

某型船用载货电梯采用PLC控制。PLC单独使用时,在控制装置在使用过程中存在一些不足,会出现运行不稳定、能耗大、噪音大等问题。通过加装变频器对某型船的PLC单独控制的系统进行系统的升级改造,介绍了基于PLC与变频器的电梯控制系统硬件组成方案及逻辑控制程序。仿真结果表明加装变频器后,电梯故障率降低,提高了电梯运行的安全性和可靠性。     

某型船电网振荡的原因分析及对策

本文分析了某型船电力系统在电网振荡时各电气参数变化的特点及电网发生振荡的原因,阐述了处理电网振荡的方法,提出了一种新的处理方法,并提出了一些建议。     

Numerical Investigations on the Aerodynamic Performance of Wind Turbine：Downwind Versus Upwind Configuration

Although the upwind configuration is more popular in the field of wind energy, the downwind one is a promising type for the offshore wind energy due to its special advantages. Different configurations ...