人脑源性神经营养因子基因克隆及序列分析

目的　克隆人脑源性神经营养因子 (hBDNF)基因并进行序列分析。方法　提取健康成人末梢血白细胞基因组DNA作为模板 ,应用PCR技术和T 载体克隆法克隆hBDNF基因 ,筛选阳性克隆、酶切鉴定 ,并进行序列测定和分析。结果　DNA序列测定的结果与GenBank提供的已知序列 (M6 1 1 81 )比较 ,所克隆的hBDNF基因从起始密码子ATG到终止密码子TAG全长共744bp ,序列完全相同。结论　自人基因组DNA中克隆hBDNF基因 ,为进一步开展阿尔茨海默病 (AD)的基因治疗积累了资料。     

EXPRESSING HUMAN MATURED BRAIN-DERIVED NEUROTROPHIC FACTOR GENE IN E. Coli AND DETERMINING ITS BIOACTIVITY

Brain-derived neurotrophic factor (BDNF)is amember of the family of neurotrophins['J. The hu-man BDNF gene is localized on chromosome of1lp13. The nucleotide sequence that is 744bpIength including signal sequence and prosequenceshows an open reading frame to encode a precursorof 247 amino acids. The matured BDNF consists ofll9 amino acids (relative molecular mass of 14Kd,PI~l0)['.'j. The activity of BDNF in the nervoussystem is widespread. BDNF could support the sur-vival of cranial …     

长缨剑胆舒——中国船级社走过45年

无论世界怎么变化,一种传统的交流手段始终影响着人们的生活--那就是船舶和航运. 占地球表面积约71%的海洋将陆地分割,这就决定了各国之间的物质交流必须依靠海上交通.统计表明,国际贸易的90%的份额由海上船舶运输.而承担海上风险管理的公益性机构--船级社,则是船舶在浩瀚海洋安全航行的有力保障.     

用P-Y曲线法计算板桩结构

根据P-Y曲线法原理分别对沉入砂性土地基及粘性土地基的板桩码头进行了例题计算,并且与传统的m法进行了比较。     

Geometric calibration algorithm of polarization camera using planar patterns

Polarization pattern provides additional information besides spectral signatures. It can be used in many applications, such as navigation, defect detection and object identification. A novel polarization camera composed of four synchronized cameras is proposed, and it can realize real-time polarization measurement. This study particularly concentrates on the geometric calibration of the system. The projection model is analyzed and the multi-camera calibration algorithm is proposed. Firstly, each camera is calibrated separately using planar patterns, and then the geometric calibration algorithms are performed. Due to the geometrical constraint, a global optimization method results in smaller estimation uncertainties. A mean rotation error of 0.025° and a mean translation error of 0.26mm are achieved after geometric calibration. The images are rectified to establish a correspondence among cameras and are combined to acquire the polarization measurement. The polarization pattern of the skylight is measured by the system and the results are consistent with the previous studies.     

Uncertainty analysis of value for money assessment for public-private partnership projects

In recent years, private sectors are encouraged to take an active part in franchising of urban infrastructure investments and operations, which promotes the rapid development of public-private partnership (PPP) in infrastructure and public service supply. Value for money (VFM) assessment has been officially proposed to provide a reference for selection of projects planning to adopt PPP. Based on the bottlenecks of VFM application in China and the uncertainties for urban infrastructure PPP projects, a discounted cash flow (DCF) model is established for VFM of infrastructure PPP projects. Then, a Monte Carlo simulation model is established on the basis of uncertainty factors for VFM. Through the analysis of Huai’an trams PPP project, coping strategies of uncertainties for VFM are put forward. Findings of the research may propel the establishment of a complete VFM evaluation system for PPP projects. Key instructional functions of VFM during the process of decision-making can be brought into full play and PPP may develop orderly.     

人工湖旁修建浅埋富水隧道暗挖技术

通过一个在湖旁用暗挖法修筑的浅埋富水隧道的实例,阐述在湖旁修筑浅埋富水地下隧道的暗挖技术。     

多年冻土路基水分迁移热力学性能分析

从水分场与温度场相互作用的角度,分别建立温度场单场控制方程和水热耦合效应的控制方程,应用有限元方法进行计算。通过对单因素温度场的计算结果与水热耦合作用下的温度场计算结果比较,分析水分迁移作用对路基温度场的影响。     

船用多电机转子磁链电流模型仿真分析

针对大多数船用感应电机采用一台变频器控制一台电机,成本较高的问题,本文提出了用一台变频器控制两台电动机的方案,从而降低成本.首先以转子磁链的电流模型和单电机控制方法为基础,并利用Matlab中的Simulink软件构建了一台变频器控制两台并联异步电动机的模型.然后使用ODE45算法,对两台并联的异步电动机进行仿真分析....     

基于模糊逻辑控制策略的混合动力汽车仿真研究

以燃油经济性和排放性能为主要控制目标,提出一种基于模糊逻辑的能量分配控制策略,应用ADVISOR仿真软件,对制定的控制策略在不同的道路循环工况下进行仿真。仿真结果表明,所设计的模糊逻辑控制策略能够合理分配发动机和电机的转矩,可使发动机工作在效率较高、排放较低的中等负荷区,整车的燃油经济性较好、排放较低。