论经济新常态下交通运输行政执法体制改革

交通运输是国民经济基础性行业,经济新常态下改革交通运输执法体制是贯彻中央全面深化改革、全面依法治国方针的内在要求,是消除执法体制机制弊端、根治执法乱象、破解交通运输发展瓶颈的有效途径,改革的主要内容和目的主要有整合职能、精简机构、核定编制、保障经费四个方面。改革中市辖区不设置执法机构,市管公路治超站移交县级管理,妥善进行富余人员转岗分流,要制定配套政策、采取措施保障改革顺利进行,处理好改革与稳定的关系,做到改革与治理两不误。     

船舶小组立装焊作业单元CPS系统研究与应用

当前船厂小组立装焊作业过程存在作业任务信息无法直接传递至现场,现场实际执行的状态信息无法及时反馈等问题,导致装焊作业混乱、装焊效率低下.为了解决上述问题,针对船舶小组立装焊作业单元进行研究,构建基于信息物理融合(CPS)的智能制造系统.首先分析了小组立装焊作业流程;然后梳理了装焊作业任务数据,构建了小组立装焊作业数据模型,并在此基础上,完成了基于物联网的CPS框架设计以及系统功能开发,实现了对装焊作业过程的有效监控;最后将该系统应用于某船厂小组立装焊现场,有效规范了装焊作业过程,提高了装焊效率.     

基于下游大水位变率的下水式垂直升船机随动技术的设计与实现

下游航道水位变率大,快速适应水位变化的准确停位随动技术,对下水式升船机的传动控制很重要.本文介绍下水式垂直升船机随动控制技术.首先,对控制对象的特性进行分析,描述了系统的速度运行曲线,对位置控制功能进行了研究.然后,搭建试验平台对本控制方案的性能进行了测试,给出了测试的方法和试验结果.实验结果表明本控制系统具有定位精度高、响应速度快等优点,能够适应下游大水位变化率的下水式垂直升船机准确停位要求.     

A proposed vision and vehicle-to-infrastructure communication-based vehicle positioning approach

It is essential to obtain accurate location of vehicles for new applications of Intelligent Transportation Systems. To remedy the defects of present Global Positioning System and vehicle-to-infrastructure (V-I) positioning technology, a new positioning approach based on vision and V-I communication is proposed. This approach aims at lane-level positioning with lower cost than conventional ones. In this approach, the position of the vehicle is represented by its lateral position (the lane number) and longitudinal position (the distance from entrance of the road) in a course coordinate system along the road; the specific lane the vehicle is occupying (the lane number) can be judged using the information of lane lines detected by vision systems; then the distance to the vehicle is obtained by a Road Side Unit (RSU) during the V-I communication; and the longitudinal position is calculated. The error of the approach on typical operating conditions is analyzed, indicating that the new approach can achieve the accuracy of less than 0.31 m for straight road and 0.58 m for typical arc road with ultra-wideband communication and ranging technologies and rational arrangement of RSUs. The feasibility of this approach is presented.     

Approximate optimal AUTOSAR software components deploying approach for automotive E/E system

The AUTOSAR has been developed as the worldwide standard for automotive E/E software systems, making the electronic components of different suppliers to be employed universally. However, as the number of component-based applications in modern automotive embedded systems grows rapidly and the hardware topology becomes increasingly complex, deploying such large number of components in automotive distributed system in manual way is over-dependent on experience of engineers which in turn is time consuming. Furthermore, the resource limitation and scheduling analysis make the problems more complex for developers to find out an approximate optimal deploying approach in system integration. In this paper, we propose a novel method to deploy the AUTOSAR components onto ECUs with the following features. First, a clustering algorithm is designed for deploying components automatically within relatively low time complexity. Second, a fitness function is designed to balance the ECUs load. The goal of our approach is to minimize the communication cost over all the runnable entities while meeting all corresponding timing constraints and balancing all the ECUs load. The experiment results show that our approach is efficient and has well performance by comparing with other existing methods in specific and synthetic data set.     

小直径盾构施工中管片纵向应力监测研究

为了探索小直径盾构法隧道在施工过程中管片纵向应力的变化规律,对北京槐房再生水厂污水隧道管片纵向应力进行了现场监测:将第976环、第1 054环管片分别设为第1和第2监测断面,2监测断面各预埋5个纵向应力计,各监测断面从本监测断面管片安装后即开始监测,当盾构掘进至第1 129环时停止监测。研究表明:1)在管片离开盾尾50环后,其纵向应力波动值小于管片拼装期间应力值的5%。2)在盾构掘进期间,管片距离盾构越远,其纵向压应力值越小。3)在管片拼装期间,管片距离盾构越远,其纵向压应力经历了先增大后减小的过程。4)管片距离盾构108环后,该管片纵向压应力趋近于0.2~0.3 MPa。5)随着盾构推进,管片纵向应力经历了4个阶段的变化过程,即周期性剧烈波动阶段—动态稳定阶段—逐渐衰减阶段—趋于稳定阶段。     

公路客运市场退出影响因素实证分析

通过对广东省公路客运企业和政府主管部门的问卷调查,采用因子分析方法,从影响客运市场退出的相关主体及相关行为中筛选出关键变量．运用非参数检验过程,就各关键变量对退出的影响程度进行比较,最终得出驾驶员和承包经营人的相关行为对公路客运市场退出最具影响的结论,为政府部门进行行业管理和政策制定提供了实证依据．     

Apoptin原核表达载体的构建及在大肠杆菌中的表达

目的构建Apoptin的原核表达载体,并制备抗原物质Apoptin融合蛋白。方法在获得Apoptin融合基因的基础上,成功构建了Apoptin的高效原核表达载体pET-28a( )-Apoptin,将该质粒转化至大肠杆菌E.coliBL21(DE3)受体菌中,以IPTG对其进行诱导表达,聚丙烯酰胺凝胶电泳分析目的蛋白。结果转化有Apoptin的原核表达载体pET-28a( )-Apoptin的大肠杆菌E.coliBL21(DE3)经IPTG诱导后,经SDS-PAGE分析,在相对分子质量约17 000的位置出现目的蛋白条带,大小与Apoptin融合蛋白一致。结论Apoptin原核表达载体pET-28a( )-Apoptin能够表达出Apoptin融合蛋白,为进一步的Apoptin研究和制备Apoptin抗体奠定了基础。     

幽门螺杆菌尿素通道蛋白基因UreI载体的构建、融合表达及纯化

目的构建幽门螺杆菌(H.pylori,Hp)尿素通道蛋白基因UreI的融合表达载体,并在E.coliBL21中表达,为进一步研究UreI的功能奠定基础。方法利用分子克隆技术以Hp DNA染色体为模板,扩增Hp UreI基因片段,将目的基因UreI与载体pET32a( )分别经kpnⅠ和HindⅢ双酶切,纯化,连接。筛选阳性重组载体,转化大肠杆菌BL21,以IPTG诱导表达pET32a( )/UreI融合蛋白。表达产物经Ni-NTA琼脂糖树脂纯化后,用SDS-PAGE及Western blot分析。结果经酶切、测序分析表明,插入的基因片段为Hp UreI编码基因,由585 bp组成,与GenBank相应菌株HP AM417 609序列完全一致,无碱基突变。经SDS-PAGE分析显示重组质粒pET32a( )/UreI在原核细胞中得到了高效融合表达,其重组融合蛋白分子量为130 ku,经Ni-NTA琼脂糖树脂纯化后,其纯度达95%以上,Western blot分析显示,该重组融合蛋白可被6-His鼠抗单克隆抗体所识别,具有良好的反应原性。结论成功地克隆和构建了原核表达载体pET32a( )/UreI,并在原核细胞中得到了高效融合表达。     

库区公路边坡稳定性分析及其防护处治

库区公路由于其工程环境的复杂性,其稳定性不但受到岩土性质、边坡形式的影响,还受到库区水位不断涨落的影响,处治不当极易发生失稳。对影响库区公路库岸边坡稳定性的主要因素进行分析,水成为制约其稳定性的关键因素,并提出相关的防护处治措施,建议采用综合治理的方案。