Application of digital image correlation method to in-situ dynamic strain measurement

Application of digital image correlation (DIC) method to determination of in-situ dynamic strain is presented in this study. Firstly, an integrative software is programmed based on the DIC algorithms and point-wise least-squares fitting technique. Then, simulated speckle images are generated to study the computational accuracy of this software. The experimental setup and procedures for measuring in-situ dynamic strain through both DIC and strain gauge are proposed. The DIC results are close to those measured by strain gauge. This fact reveals that DIC is a practical and effective tool for in-situ dynamic strain measurement. Finally, the full-field in-situ dynamic strain of another specimen is measured by DIC, and the overall distribution of the strain in the measurement area is clearly shown.     

基于PANDA软件的框架网坐标解算可靠性分析

应用高精度定位解算软件PANDA对框架网进行解算,并与Bernese解进行比较,探讨不同软件模型下框架网的点位坐标解算方法及其精度;通过算例表明PANDA软件的定位解算结果与Bernese的解有较好的一致性,表明PANDA软件有较高的可靠性和稳定性。     

乘用车进气系统核心试验方法解读

通过对进气系统在零部件系统试验、发动机台架试验、整车试验上的核心试验方法解读,让读者对进气系统有更全面的认识。     

Simulation of mixed gas formation for a spray-wall complex guided LPG direct injection engine

To obtain an ultralean air-fuel ratio and to reduce engine-out NOX and HC emissions induced by the richer mixture near the spark plug, a spray and wall complex guided combustion system has been developed by utilizing the fuel characteristics of LPG. The new combustion system configuration is optimized by using a commercial CFD code, FIRE V2013, and the reliability of the system has been experimentally demonstrated by Plane Laser-Induced Fluorescence (PLIF). The mixture formation in the new combustion system under part load (2,000 rpm) is numerically simulated. With an injection timing of 40°CA BTDC, the LPG spray which is injected from two upper holes, reaches the ignition point, and the other part of the LPG spray which is injected from the bottom hole, is directed to the ignition point through the vertical vortices at the same time. At the ignition timing of about 20°CA BTDC, the two-part mixtures have been shown to form a stable and richer stratified mixture around the ignition point, and the maximum global air-fuel ratio reaches to 60: 1.     

论个人所得税税制模式改革

文中介绍了现行个人所得税中存在的问题,提出进行“综合征收模式”改革的必要性、合理性以及实行条件。     

大型活动交通影响评价方法

大型活动吸引和产生的突增交通量会造成周边地区的交通拥挤。通过比较大型活动与大型商业建设项目的交通影响评价特点和分析大型活动交通管理的影响因素,分别针对“提供一次性服务的大型活动”和“提供长效服务的大型活动”,设计了交通需求的预测方法。以交叉口服务水平分析方法为基础,提出一种了大型活动的交通影响评价方法。以长春市南岭体育场举办的“中国足球甲A联赛”为对象,运用Vissim微观交通仿真软件对所提出的方法进行了初步验证。结果表明,在大型活动条件下,所提出的方法能够确定需要进行交通管制的空间范围。     

新加坡公共交通发展经验及启示

通过实际考察和走访咨询,系统地总结了新加坡的公共交通发展经验。首先介绍了新加坡对小汽车和公共交通发展采取的＂推、拉策略＂,以及新加坡公共交通的发展历程。在此基础上,对新加坡公共交通发展的成功经验进行了归纳和总结,包括管理机制、公共交通设施建设、公共交通运营系统、政策法规等方面的内容。结合我国城市交通发展的现状和特点,对城市公共交通的发展提出了措施和建议。     

A data model applied to the knowledge-based ICAS

Image-guided computer aided surgery system (ICAS) contributes to safeness and success of surgery operations by means of displaying anatomical structures and showing correlative information to surgeons in the process of operation. Based on analysis of requirements for ICAS, a new concept of clinical knowledge-based ICAS was proposed. Designing a reasonable data structure model is essential for realizing this new concept. The traditional data structure is limited in expressing and reusing the clinical knowledge such as locating an anatomical object, topological relations of anatomical objects and correlative clinical attributes. A data structure model called mixed adjacency lists by octree-path-chain (MALOC) was outlined, which can combine patient's images with clinical knowledge, as well as efficiently locate the instrument and search the objects' information. The efficiency of data structures was analyzed and experimental results were given in comparison to other traditional data structures. The result of the nasal surgery experiment proves that MALOC is a proper model for clinical knowledge-based ICAS that has advantages in not only locating the operative instrument precisely but also proving surgeons with real-time operation-correlative information. It is shown that the clinical knowledge-based ICAS with MALOC model has advantages in terms of safety and success of surgical operations, and help in accurately locating the operative instrument and providing operation-correlative knowledge and information to surgeons in the process of operations.     

幽门螺杆菌尿素通道蛋白基因UreI载体的构建、融合表达及纯化

目的构建幽门螺杆菌(H.pylori,Hp)尿素通道蛋白基因UreI的融合表达载体,并在E.coliBL21中表达,为进一步研究UreI的功能奠定基础。方法利用分子克隆技术以Hp DNA染色体为模板,扩增Hp UreI基因片段,将目的基因UreI与载体pET32a( )分别经kpnⅠ和HindⅢ双酶切,纯化,连接。筛选阳性重组载体,转化大肠杆菌BL21,以IPTG诱导表达pET32a( )/UreI融合蛋白。表达产物经Ni-NTA琼脂糖树脂纯化后,用SDS-PAGE及Western blot分析。结果经酶切、测序分析表明,插入的基因片段为Hp UreI编码基因,由585 bp组成,与GenBank相应菌株HP AM417 609序列完全一致,无碱基突变。经SDS-PAGE分析显示重组质粒pET32a( )/UreI在原核细胞中得到了高效融合表达,其重组融合蛋白分子量为130 ku,经Ni-NTA琼脂糖树脂纯化后,其纯度达95%以上,Western blot分析显示,该重组融合蛋白可被6-His鼠抗单克隆抗体所识别,具有良好的反应原性。结论成功地克隆和构建了原核表达载体pET32a( )/UreI,并在原核细胞中得到了高效融合表达。     

城市老城区健康道路交通模式探讨

为解决城市老城区交通压力持续增大的问题,从道路系统、公共交通系统、慢行交通系统三个方面进行研究,基于健康城市理念,并借鉴国外城市老城区交通发展模式,提出国内城市老城区健康道路交通模式应具有道路网络"高密度、小街区、微循环",公共交通全覆盖、高效率,慢行交通空间连续、设施完善、与公共交通无缝衔接等特征,为老城区健康道路交通规划设计提供参考。