跨既有铁路钢桁梁拖拉施工技术

山西中南部铁路通道采用96 m钢桁梁跨越京沪铁路,施工采用整体拼装后,采用钢梁前端不设置导梁,在两个主墩间增设临时支墩施工技术,完成拖拉施工。以此为例探讨了跨越既有铁路架设钢桁梁的施工技术。     

Microstructures and mechanical properties of various Mg-Li wrought alloys

Three kinds of ultra-light Mg-Li wrought alloys,Mg-5.5Li-3Al-1Zn-1Ce,Mg-7.0Li-3Al-1Zn-1Ce and Mg-10.5Li-3Al-1Zn-1Ce,were prepared by vacuum induction melting furnace;then the alloys were extruded and rolled.The microstructures and properties of these alloys were studied.The results indicate that the Mg-5.5Li3Al-1Zn-1Ce alloy has the highest strength,the Mg-10.5Li-3Al-1Zn-1Ce alloy has maximum elongation,and the Mg-7.0Li-3Al-1Zn-1Ce alloy has good comprehensive performance.Rolling deformation can increase strength further after extrusion deformation.     

Durability of concrete made with manganese slag as supplementary cementitious materials

This paper discusses mineral composition and pore microstructure characteristics of water-cooled manganese slag and its effects on durability of concrete. The Mn slag has an alveolate pore structure, and the ground Mn slag is characterized by multiangular shape which consists of α′-C₂S, C₃MS₂, CaO·MnO·2SiO₂ and C₂AS. Experimental results show that the Mn slag has potential hydraulic reactivity. Concrete made with Mn slag as supplementary cementitious materials (SCMs) exhibits very low strength loss and weight loss in the synthetic seawater corrosion and freezing-thawing cycle tests. The research provides useful reference for knowing about Mn slag and for applying Mn slag to improve the durability of concrete.     

Novel material of hydrogen generation

A novel aluminum alloy which could react with water and generate hydrogen at room temperature was prepared via mechanical alloying (MA). The results shows that milling time, additives and mass ratio have a significant role in the hydrogen production rate. The highest hydrogen production rate of alloys reaches 95% of theoretical value. The velocity of hydrogen generation reaches more over 200 mL/min for a gram alloy. Active materials (Ga and In) included in the aluminum alloys can be recycled and used repeatedly. The hydrolysis reaction between aluminum and H₂O will take place and release a large amount of heat, which contributes to increasing the velocity of hydrogen generation. The reaction products consist of AlO(OH) and hydrogen.     

Biocompatibility evaluation of polyethylene terephthalate artificial ligament coating hydroxyapatite by fibroblasts cells in vitro

Hydroxyapatite (HA) based materials have been widely used in the field of ligament tissue engineering in the past decades. It has been previously reported that HA can increase the penetration of marrow-derived mesenchymal stem cells (MSCs) and MSCs cells into scaffolds due to increased cell differentiation in biological media. Additionally, it was found that there are much difference between MSCs and anterior cruciate ligament (ACL) cells. For that reason, we mainly evaluate the biocompatibility of polyethylene terephthalate (PET) silk scaffold with fibroblasts cells in vitro. We cultured mouse fibroblasts cells on the substrate of PET fiber and PET-HA scaffold, respectively, and then observed the morphology by using scanning electron microscopy. Our data indicate that PET-HA scaffold has good biocompatibility with fibroblasts cells and can potentially be useful in enhancing the fibroblasts cell differentiation and proliferation.     

地铁盾构施工对临近通信发射塔的工后影响分析

地铁盾构施工会使周边临近构筑物产生不均匀沉降和倾斜,对构筑物结构的稳定性产生不良影响.北京地铁某盾构区间近距离下穿铁路通信发射塔一年后,经检测发现发射塔基础产生了较大不均匀沉降.基于该铁路通信发射塔,结合工前、工后（施工1年后）检测结果及施工过程监测数据,采用有限元数值模拟分析方法,考虑地铁盾构施工造成的结构不均匀沉降及结构初始差异沉降,综合分析盾构近距离施工对通信发射塔结构稳定性的影响,评价通信发射塔的安全状况.研究表明：盾构隧道施工所产生的不均匀沉降造成的通信发射塔结构变形在安全容许范围内,结构变形均能满足发射塔材料安全性要求,通信发射塔基础东北角与东南角塔基承载力安全系数较低,需对其采取加固措施.     

拟“车-桥耦合系统”的动态近似分析法

目前对于车体通过桥梁的动力响应问题,通常是将车辆子系统和桥梁结构耦合为一个系统,同时进行分析.文中提出一种近似但具有足够精度的方法用以计算车-桥耦合系统的动力响应.将车-桥耦合方程分解为两个部分,忽略作用在桥梁上的由移动车辆产生的惯性力项,只剩下移动力项.计算出桥梁在移动力作用下的响应,并把此响应作为车体动力响应的激励源反馈给车体,从而计算出车体的响应.从数值计算的角度,采用MATLAB编程并与车桥耦合系统精确的数值解作对比,验证了轻车过重桥情况下本文方法的可行性.通过分析表明,此方法能避免车-桥耦合系统计算时的庞大运算.     

动态瞬时心率抗干扰检测算法设计

为了采集准确的心电信号,设计了一套基于瞬时心率信号抗干扰测量的方法,针对微弱并易受干扰的心电采样信号,进行有效地硬件以及软件滤波,通过软件数字滤波算法,得到真实的心率信号．实验结果表明,当运动状态稳定时,通过对采样信号脉宽限制及16个采样数据做滑动平均处理,得到的数据能够显示人体运动时的真实心率,数据波动正确的反映了心率状态,能够提供稳定准确的数值依据．     

人骨髓间充质干细胞生物学特性及腺病毒介导的GFP标记

目的建立人骨髓间充质干细胞(hMSCs)分离、培养以及鉴定的方法,同时应用腺病毒介导的绿色荧光蛋白(Ad-GFP)检测细胞转染的效率。方法应用人淋巴细胞分离液(Ficoll)对hMSCs进行分离,经贴壁纯化后用MTT法检测增殖能力;并对其成骨、成脂、成平滑肌诱导分别进行碱性磷酸酶(ALP)、油红O染色及α-SMA免疫细胞化学检测;采用流式细胞仪(FCM)检测细胞表面标志的表达。用Ad-GFP转染,并应用荧光显微镜与FCM检测转染效率。结果 hMSCs镜下为成纤维样形态,约在第4天进入对数增长期;分化诱导后ALP染色、Von kossa银染、油红O染色以及α-SMA均为阳性;FCM检测CD14、CD31、CD45表达阴性,CD44、CD73、CD106及PDGFRβ表达阳性;当感染复数MOI=200,细胞培养48h时感染效率较高,FCM检测显示为99.81%。结论经过对增殖、分化能力以及表面标志的分析,证明用Ficoll可以分离得到较纯的hMSCs,Ad-GFP可以很好地感染细胞,为组织工程种子细胞的体内示踪提供了途径。     

热疗对膀胱癌细胞BIU-87/HCPT耐药相关基因的影响

目的观察热疗对耐羟基喜树碱膀胱癌细胞株BIU-87/HCPT多药耐药的逆转作用,并探讨其逆转肿瘤多药耐药的机制。方法不同温度条件下热疗、化疗、热疗联合化疗处理耐药膀胱癌细胞株BIU-87/HCPT后,用MTT法检测细胞增殖,流式细胞仪检测细胞周期、细胞凋亡、P-gp、多药耐药相关蛋白1(MRP1)、谷胱甘肽硫-转移酶π(GST-π)和survivin表达的变化。结果热疗对BIU-87/HCPT细胞具有抑制作用,呈温度依赖性且在43℃时最强。热疗可显著增加羟基喜树碱对BIU-87/HCPT细胞的抑制率,作用呈温度依赖性,热疗明显增加化疗的作用。热疗联合化疗明显减少P-gp、MRP1、GST-π和survivin的表达并呈温度依赖性。结论热疗可促进膀胱癌细胞株BIU-87/HCPT凋亡,增强化疗药物作用,部分逆转膀胱癌细胞的耐药性,减少P-gp、MRP1、GST-π和survivin的表达。