氧化苦参碱对慢性肾脏病患者血清TGF-β1和Col Ⅲ的影响

目的观察氧化苦参碱对慢性肾脏病(CKD)患者血清转化生长因子-β1(TGF-β1)、Ⅲ型胶原(ColⅢ)的影响,探讨其在肾间质纤维化发生发展过程中的作用和治疗价值。方法选取健康对照者10例、慢性肾脏病患者40例,根据肾小球滤过率(GFR)分为轻度肾损害组(GFR<90 mL/min.1.73 m2,CKDⅠ)、中重度肾损害组(GFR<60 mL/min.1.73 m2,CKDⅡ),再分层随机抽样,分为常规治疗组和氧化苦参碱组。利用双抗体夹心酶联免疫吸附法分别检测各研究对象治疗前、后血清TGF-β1、ColⅢ含量,采用t检验比较两组TGF-β1、ColⅢ水平的差异。结果氧化苦参碱组TGF-β1、ColⅢ含量显著低于常规治疗组(P<0.05)。结论氧化苦参碱可能通过下调TGF-β1、ColⅢ水平,减少ECM沉积,从而达到防止肾间质纤维化的作用。     

刀豆蛋白A条件化培养基对体外培养的兔角膜内皮细胞生长的影响

目的研究刀豆蛋白A(concanavalin A,ConA)条件化培养基对体外培养的兔角膜内皮细胞(rabbit corneal endothelial cells,RCECs)生长的影响。方法采用揭取后弹力层及内皮细胞联合组织块法进行RCECs的体外原代培养,分别用含有体积分数为5%、10%、15%、20%ConA的活化大鼠脾细胞条件上清液的RPMI1640培养基进行RCECs原代培养,并用含10%(体积分数)胎牛血清(fetal bovine serum,FBS)的RPMI1640培养基作为对照。神经烯醇化酶免疫组织化学染色法进行细胞鉴定;噻唑蓝比色法观察各组RCECs生长的状况;SPSS11.0One-way ANOVA及LSD检验进行统计分析。结果成功进行了RCECs的体外培养,培养细胞内神经烯醇化酶阳性表达。ConA条件化培养基各组细胞增殖均高于对照组(P<0.001),且10%、15%ConA条件化培养基组的RCECs细胞数量明显高于其他各实验组(P<0.001)。结论ConA条件化培养基具有促进RCECs生长的作用,可作为培养辅佐剂。     

动力总成悬置支架多目标拓扑优化和强度分析

基于Optistruct,以支架的最小柔度、最大固有频率作为优化目标函数;以应力、体积作为约束函数,对一发动机后拉杆安装支架进行拓扑优化,优化结果表明:在满足悬置支架强度和模态要求下,质量减少了12%。     

Proteus在电气信息类实验教学改革中的应用

针对电气自动化专业人才培养的特点,提出了Proteus仿真软件在电气信息类实验教学改革中的应用。通过Proteus软件在模拟电子技术、数字电子技术以及单片机课程中的应用举例表明,该教学改革方案不仅能提高实验教学质量,还能提高学生的创新思维和学习能力。     

冰区航行船舶电力推进轴系机电耦合的扭振分析

通过螺旋桨桨叶与冰块的相互作用过程,建立单冰块对螺旋桨扭转振动的激励函数;同时建立机电耦合振动数学模型,推导得到机电耦联动力学方程,研究电机电磁激励力对扭转振动的影响。以3500吨科考船电力推进轴系为研究对象,基于冰载荷激励力和电磁激励力对轴系扭转振动进行分析,为复杂的船舶电力推进轴系在冰区航行时的振动研究奠定了基础。     

Marine accident analysis for collision and grounding in oil tanker using FTA method

In this study, collision and grounding data registered in GISIS (Global Integrated Shipping Information System) were investigated for oil tankers. The database includes the information of the collision and grounding accidents during the period between 1998 and 2010 in oil tankers. The risk assessments were carried out using fault tree analysis (FTA) programme for the incidents as collision and grounding occurred in oil tankers. In this study, we were able to investigate first the potential problems which cause the collision and grounding accidents have been determined, second, the occurrence of accidents has been shown with causal factors by the FTA method, and, finally, the significance degree of the initial events causing occurrence of accidents have been put forth. Collision in oil tanker resulted in economical loss (81%), pollution (6%) and death or injury (13%). Grounding in oil tanker resulted in economical loss (91%) and pollution (9%). According to the FTA results, the main reason for the accidents originating from human error is as follows: for collision accidents, Convention on the International Regulations for Preventing Collisions at Sea (COLREG) violation and the lack of communication between vessels; and for grounding accidents, the interpretation failure of the officer on watch and lack of communication in the bridge resource management.     

舰艇结构在水中兵器静态爆炸作用下的冲击响应

运用通用有限元程序ABAQUS对水中兵器静态爆炸攻击作用下的舰艇冲击响应进行数值模拟分析,并数值模拟出舰艇关键部位的冲击响应特征,成功地解决了流-固耦合和单元失效等关键问题,数值模拟出水域流场压力、舰艇应力响应和加速度响应的结果。数值分析获得的舰艇在水中兵器静态爆炸试验工况下的响应结果与理论计算结果基本相符,具有较高可信度,为水中兵器静态爆炸试验工况的设置和舰艇抗爆结构的设计提供有力的依据。     

Transportation infrastructure improvement and real estate value: impact of level crossing removal project on housing prices

This paper studies the impact of removing the level crossing, which constitutes traffic hazard to the society, on house prices by conducting a quasi-natural experiment using the Level Crossing Removal Project (LXRP) implemented by the Victoria state government in Australia since 2015. Using a difference-in-differences method, we analyzed the changes in housing prices due to the improvement of transportation infrastructure, gauging the LXRP’s impact on house and unit submarkets separately. We found that the prices for house and unit markets increased significantly after the removal of level crossings, with the value uplift decreasing with distance from the removal site. This paper contributes to the existing literature by adding an empirical study related to the enhancement of infrastructure aiming to improve the traffic safety in the urban context. Unlike previous studies, this study examines the effect of improvement projects for existing infrastructure and provides relevant implications to improve the efficiency of investing public resources in infrastructure improvement.