Factors associated with bicycle ownership and use: a study of six small U.S. cities

As a means of transportation and as a form of physical activity, bicycling generates benefits to the bicyclist as well as to the community as a whole. Bicycling now accounts for less than 1 percent of all trips for all purposes in the U.S., but evidence from other western countries suggests that under the right conditions, bicycling levels can be significantly higher. Indeed, the experiences of some U.S. cities suggest that it is possible to create conditions conducive to higher levels of bicycling even in the U.S. However, the extent to which bicycle investments have contributed to bicycling levels in these communities has not been rigorously assessed. The purpose of this study is to provide a better understanding of the determinants of bicycle ownership and use as a basis for identifying ways to promote bicycling. A cross-sectional study of six cities was designed to test the importance of bicycle infrastructure and other physical environment factors relative to individual factors and social environment factors, using a nested logit model to examine ownership and use decisions jointly. The results show strong effects of individual attitudes and physical and social environment factors on bicycle ownership and use.     

沥青混合料的集料比表面积确定方法的研究

通过对沥青混合料集料比表面积确定的传统方法进行研究,并对矿粉和集料的表面积进行了实测,以及对沥青混合料的集料表面积系数进行推导,认为现有的确定沥青混合料的集料比表面积的方法值得商榷.通过对这两种方法进行分析,并结合沥青混合料的集料比表面积的实测结果,提出了不同于常用方法的沥青混合料的集料比表面积的确定方法,并确定了集料...     

核受体免疫组织化学染色时抗原修复方法的选择

目的 探讨不同抗原修复法对DA.1U大鼠肝组织内核受体检测结果的影响.方法 应用微波修复法、高压修复法、酶修复法和高压加酶修复法对DA.1U大鼠肝组织切片进行修复,然后进行免疫组化染色.结果 DA.1U大鼠肝组织内核受体LXR-α、LXR-β、PPAR-γ和FXR经高压抗原修复后,染色阳性强度较其他修复方法明显增强,而且定位较佳.结论 在核受体免疫组化染色中高压抗原修复法染色效果优于其他抗原修复法.     

发动机冷却风扇气动性能的计算方法

介绍了发动机冷却风扇气动性能的计算方法、建模方法和求解技术。利用CFD软件Fluent计算了某款风扇的静压、功率和效率与流量的关系,试验结果验证了计算结果。通过对风扇内部压力场和速度场的研究,分析了风扇效率低下的原因,并提出了改进方案。计算结果表明,叶尖间隙对风扇性能有重要影响,叶尖间隙过大将导致风扇效率低下,故应减小叶尖间隙或安装圆环以提高风扇性能。     

Approximations to the magic formula

Pacejka’s tire model is widely used and well-known by the automotive engineering community. The magic formula describes the brake force, side force and self-aligning torque in terms of the longitudinal slip and slip angle, plus several corrections. This paper uses approximation theory to obtain different types of approximations to the magic formula: rational functions (RA) resulting from the Remez algorithm, expansions in a series of Chebyshev polynomials (ACh), a series of Chebyshev rational polynomials (ARChPs), a series of rational orthogonal functions (ORF) and a series of ARChPs that result from grade-1 ORFs. The last expansion shows the fastest convergence and most effective computation. Jacobi rational polynomials can also be obtained to complement this expansion and facilitate fine-tuning in specific areas of the error curve. This work is complemented by obtaining the original rational approximations to the inverse tangent function, which take advantage of the curve symmetry to reduce the computation load and provide models that include the influence of the vertical load. The convergence properties of the development in series and the error values resulting from numeric examples for the three types of stress are shown. The proposed final ARChP expressions show very low error (1%) compared to the original magic formula. They can be computed 20 times faster; they can be evaluated, derived and integrated analytically easily; and their coefficients can be obtained from tests using common least-squares algorithms.     

Robust lane markings detection and road geometry computation

Detection of lane markings based on a camera sensor can be a low-cost solution to lane departure and curve-over-speed warnings. A number of methods and implementations have been reported in the literature. However, reliable detection is still an issue because of cast shadows, worn and occluded markings, variable ambient lighting conditions, for example. We focus on increasing detection reliability in two ways. First, we employed an image feature other than the commonly used edges: ridges, which we claim addresses this problem better. Second, we adapted RANSAC, a generic robust estimation method, to fit a parametric model of a pair of lane lines to the image features, based on both ridgeness and ridge orientation. In addition, the model was fitted for the left and right lane lines simultaneously to enforce a consistent result. Four measures of interest for driver assistance applications were directly computed from the fitted parametric model at each frame: lane width, lane curvature, and vehicle yaw angle and lateral offset with regard the lane medial axis. We qualitatively assessed our method in video sequences captured on several road types and under very different lighting conditions. We also quantitatively assessed it on synthetic but realistic video sequences for which road geometry and vehicle trajectory ground truth are known.     

连续配筋混凝土路面纵向钢筋荷载疲劳断裂分析

采用ANSYS非线性有限元程序,分析标准轴载作用下连续配筋混凝土路面横向开裂稳定后裂缝处纵向钢筋的塑性应变,并利用损伤理论及屈服准则推导重复荷载作用下裂缝处纵向钢筋断裂的疲劳公式,具有一定的理论和现实意义。     

TGF-β诱导骨髓干细胞分化为心肌样细胞的研究

目的探讨转化生长因子β(TGF-β)对骨髓间充质干细胞(MSCs)定向分化为心肌样细胞的影响。方法取SD大鼠四肢骨骨髓,分离培养MSCs,应用TGF-β1对第二代MSCs定向诱导,相差显微镜观察细胞形态学变化,应用免疫细胞化学技术检测诱导后4周MSCs结蛋白(desmin),α-横纹肌肌动蛋白(α-sarcomeric actin)、心肌肌钙蛋白T(CTnT)及p38MAPK的表达情况,并根据CTnT这一心肌源性特异性标记物的阳性率,分析心肌细胞的转化率;同时,以半定量RT-PCR方法在诱导后7、21、28d检测细胞心肌早期转录因子GATA-4和心肌特异性肌凝蛋白重链α-MHC的表达。结果①加入TGF-β1诱导后的MSCs,于7d时形态已转变成类长柱形,28d时细胞体积则变小,呈条梭状排列,可观察到类肌管样结构。②TGF-β1诱导4周后的MSCs均可见desmin、α-sarcomeric actin及p38MAPK阳性细胞。诱导组的心肌样细胞转化率均显著高于对照组(P均<0.01)。③RT-PCR结果显示,诱导组细胞GATA-4于诱导后7d弱表达,21d表达增强,28d表达减弱。α-MHC在诱导后7d不表达,21d弱表达,28d表达明显。结论骨髓间充质干细胞在TGF-β诱导下可定向分化为心肌样细胞。     

肿瘤坏死因子α、白介素1β和舒马曲坦对大鼠三叉神经节离体培养后降钙素基因相关肽mRNA表达水平的影响

目的探讨肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)和抗偏头痛药物舒马曲坦(Sumatriptan)对大鼠三叉神经节(TG)离体培养后降钙素基因相关肽(CGRP)mRNA表达水平的影响及其细胞内信号转导机制。方法将不同浓度的TNF-α、IL-1β、IL-2和Sumatriptan分别加入DMEM培养液中,与SD大鼠TG离体孵育24h;进一步将细胞内丝裂原激活的蛋白激酶(MAPK)信号转导系统ERK1/2、JNK、P38三条信号通路关键激酶特异性阻断剂PD98059、SP600125、SB239063加入DMEM培养液中,30min后分别加入有效浓度的炎性因子TNF-α和IL-1β后,与大鼠TG共同孵育24h,实时定量PCR(RT-PCR)法检测TG内CGRP-mRNA表达水平变化。结果 50μg/L的TNF-α、25μg/L的IL-1β可明显上调离体TG内CGRP-mRNA表达水平(P<0.05),0.1g/L和0.5g/L Sumatriptan能显著下调TG内CGRP-mRNA表达水平,而20～80μg/L的IL-2对CGRP-mRNA表达无影响。特异性阻滞剂SP600125、SB239063分别与50μg/L TNF-α、25μg/L IL-1β孵育24h后,CGRP-mRNA表达量较对照组显著降低(P<0.05)。结论 TG离体培养时,炎性因子TNF-α、IL-1β可显著上调CGRP-mRNA表达水平;一定浓度的舒马曲坦能下调CGRP-mRNA表达;JNK和P38信号转导通路参与了TNF-α、IL-1β诱导的TG内CGRP-mRNA上调过程。     

3-羟基丁酸对体外培养大鼠皮质神经干细胞增殖分化的影响

目的研究体外条件下3-羟基丁酸(3-HB)对神经干细胞(NSCs)增殖及分化的影响。方法分离培养孕14.5d大鼠胚胎大脑皮质NSCs,分别以不同质量浓度3-HB(0.1、0.05、0.02、0.01g/L)处理培养至第3代的NSCs,以未加药组作为对照。CCK-8试剂盒检测细胞增殖、活力,免疫细胞化学染色观察NSCs的分化情况。结果与对照组相比,3-HB对NSCs的增殖、活力没有显著影响,但3-HB处理组NSCs分化为β-tubulinⅢ阳性细胞(神经元)的比例增加,而分化为GFAP阳性细胞(胶质细胞)的比例没有显著变化。结论 3-HB不影响NSCs的增殖,但可促进其向神经元方向分化。