新型EGFP标记的杆状病毒转移载体的构建及EGFP的制备

目的构建可表达增强型绿色荧光蛋白(EGFP)融合蛋白的昆虫杆状病毒转移载体,利用Sf-9细胞表达、制备EGFP。方法用PCR法从pEGFP质粒中扩增EGFP基因,NcoⅠ/SalⅠ双酶切,克隆入杆状病毒转移载体NcoⅠ/SalⅠ酶切位点之间,获得pFB-EGFP质粒;转化E.coliDH10Bac,通过转座子Tn7的介导,获得重组杆状病毒Ac-EGFP;感染Sf-9昆虫细胞;利用激光共聚焦显微镜和SDS-PAGE观察并检测EGFP的表达。非变性法纯化EGFP。结果荧光显微镜和激光共聚焦显微镜证实感染的Sf-9细胞可表达EGFP;通过SDS-PAGE获得的EGFP,大小为27ku。结论重组pFB-EGFP载体具备表达EGFP的能力,为分子生物学研究提供了一种新型可表达EGFP融合蛋白的昆虫杆状病毒表达载体。     

异丙酚对兔肝缺血再灌注损伤时内皮素-1和一氧化氮的影响

目的探讨异丙酚对兔在体肝缺血再灌注损伤时内皮素-1(ET-1)和一氧化氮(NO)的影响。方法40只家兔随机分为4组,每组10只,分别为缺血再灌注组(A组)、缺血再灌注加生理盐水2 mL/(kg.h)组(B组)、缺血再灌注加异丙酚8mg/(kg.h)组(C组)和假手术对照组(D组)。各组于再灌注后306、0 min测定肝组织和血浆中ET-1与NO水平以及血清谷丙转氨酶(ALT)、谷草转氨酶(AST)的水平,并进行肝组织形态学观察。结果与D组比较,A、B、C组于再灌注后30 min及60 min时,肝组织和血浆中ET-1均呈上升,但C组的上升程度明显低于同时间点的A、B两组(P<0.01);同时肝组织和血清中NO呈下降,但C组的下降程度明显低于同时间点的A、B两组(P<0.01);血清ALT、AST均升高,但C组的升高程度明显低于同时间点的A、B两组(P<0.01);肝细胞形态学发生异常改变,C组的肝脏淤血明显较A、B两组轻,肝细胞变性坏死不明显。结论异丙酚通过保护肝窦内皮,降低机体内ET-1水平,提高NO水平,改善肝脏微循环障碍,对HIRI有保护作用。     

基于频域法的结构疲劳寿命预测方法研究

以Didik方法的雨流循环计数应力范围概率密度函数为依据,考虑长寿命区的S-N曲线,利用不完全Γ函数建立了一种宽带随机载荷谱下结构疲劳寿命计算的解析解法,为用频域法计算结构在宽带随机载荷谱下疲劳寿命提供了一种快捷方法。     

Calculation of strain amplification matrix for strain invariant failure theory based on representative volume element models with periodical boundary condition

Strain invariant failure theory (SIFT) is a micro-mechanics-based failure theory for multi-scale failure analysis of composite materials originally proposed by Gosse and Christensen. In this paper, the approach for obtaining strain amplification matrix which is a key step for the execution of SIFT is improved by adopting representative volume element (RVE) finite element models considering periodical boundary condition, based on which more actual deformation mode is reflected. The deformation modes and strain data at the characteristic points of the centroid cell of multi-cell RVE model are analyzed and taken as a reference. It can be concluded that more reasonable deformation mode and relationship between the micro-mechanical and macro-mechanical strain states are obtained by employing the new model. Finally, numerical examples are provided to illustrate the determination of strain amplification factors within the RVEs considering periodical boundary condition at the characteristic points.     

Target priority determination methods by interval-valued intuitionistic fuzzy sets with unknown attribute weights

The paper aims at the problem of multi-targets threat degree being hard to be evaluated accurately in complex air defense battlefield environments. Combined with multi-sensors information fusion and interval-valued intuitionistic fuzzy sets (IVIFS) theories, the target priority determination is studied. The score and accuracy functions of IVIFS are improved with thinking about the hesitating information in order to increase the rationality. Then, the influence factors of target priority and the nonlinear relationship between the influence factors and target priority are analyzed. Next, the algorithms for calculating the factor weights and sensor weights are given. Based on the theory of IVIFS and technique for order preference by similarity to an ideal solution (TOPSIS), two methods of target priority determination based on the IVIFS and TOPSIS are proposed. At last, an application example verifies the effectiveness and flexibility of the proposed algorithms.     

关于船舶UPS的容量估算

伴随着不间断电源(UPS)技术在船舶以及海洋工程项目中的使用日益广泛,人们对不间断电源技术的了解也日益增加。该文通过实船案例结合理论知识详细介绍UPS的工作方式以及UPS的容量确定、蓄电池容量确认等内容。     

限制性Ⅲ级航道船舶下沉量研究

简要分析了影响内河船舶下沉量的主要因素,介绍了国内外关于船舶下沉量的研究概况及相关近似计算公式.根据Ⅲ级航道船舶下沉量测试物理模型试验结果,提出了适合内河限制性Ⅲ级航道的船舶下沉量近似计算公式.计算结果与模型试验结果对比表明,该公式达到了较高的计算精度,对航道水深计算及航道断面设计具有重要的参考价值.     

基于批量分割的虚拟单元制造系统多阶段动态调度

鉴于虚拟单元中批量分割调度模型研究的不足以及虚拟单元下批量分割呈现的新特点,文中考虑作业车间中同类设备有多台,多重作业具有不同加工路径可选的情况下的批量调度问题,其中批量分割包含横向分割和纵向分割。考虑到调度的复杂性,文中运用一个多阶段调度模型对设备资源进行分配,确定设备上的批量种类、大小和顺序,并在交货期内使总生产成本达到最小。通过算法对案例研究结果的对比,证明了所提方法的可行性和有效性。     

In situ colonization of marine biofilms on UNS S32760 duplex stainless steel coupons in areas with different water qualities: Implications for corrosion potential behavior

In the presence of biofilms, stainless steels （SS） exhibits an increase in corrosion potential, called ennoblement. In the present study, the corrosion potential （Eco,） behavior of the duplex SS UNS S32760 was recorded simultaneously with the in situ marine biofilm formation in two areas at Arraial do Cabo, Southeastern Brazil. The biofilm at Forno Harbor （an anthropogenically disturbed area） was characterized by higher relative abundances of Bacteria at day 2, followed by diatoms （especially Navicula sp.） on day 10 and dinoflagellates on day 18, whereas no clear trend was recorded at Cabo Frio Island （an undisturbed area）. The ennoblement of Ecor values was site-dependent. In a complementary laboratory assay, biofilms were removed and the Eco values registered in sterile conditions for the subsequent 10 days and corroborated in situ results. Understanding biofilms and SS interactions has important implications for materials science and engineering decisions as well as helping to fill in imnortant gaps in this knowledge.     

The Teachable Moments on Marine Mammal Tours: Watching versus Swim-With Tours

With the continuing growth of the tourism industry over recent decades, has come a change in tourist behavior from the traditional stereotypical 3-S tourist (sun, sand, sex) to an increasingly sophisticated and demanding tourist. These “new” tourists like to learn about history, nature, and wildlife, and they expect an educational component on wildlife tours. This study investigated swim-with-dolphin tours in Kaikoura, New Zealand with regard to the educational content and the overall anatomy of these tours, using data from observations on a total of 32 tours during December 2011. The resulting data were then compared with the data from Hrycik and Forestell's study, which investigated the content of questions asked by passengers on whale-watching tours in Stellwagen Bank National Marine Sanctuary. Results of the on-tour observations indicate that the surveyed tours display a structured approach to interpretation, which is customized to the specific tour anatomy of a “swim-with” tour, and varies in some parts significantly from “watch-only” tour interpretation.