液货舱透气系统设计与计算方法研究

针对IMO通函以及ISO标准的新要求，本文从船东、船厂和船舶设计院所应履行职责的角度，对新建油船和化学品船透气系统的设计与计算方法进行研究，并给出了实例。     

工业机器人在我国汽车生产中应用的技术分析

本文对汽车生产中采用机器人的应用领域进行了技术分析,就合理的选型、适宜的环境、完善的维护、恰当的布局等问题进行了深入的讨论。并指出应加强机器人技术的应用研究以适应未来10年我国汽车工业大发展需要。     

Carbody elastic vibrations of high-speed vehicles caused by bogie hunting instability

In particular locations of the high-speed track, the worn wheel profile matched up with the worn rail profile will lead to an extremely high-conicity wheel–rail contact. Consequently, the bogie hunting instability arises, which further results in the so-called carbody shaking phenomenon. In this paper, the carbody elastic vibrations of a high-speed vehicle in service are firstly introduced. Modal tests are conducted to identity the elastic modes of the carbody. The ride comfort and running safety indices for the tested vehicle are evaluated. The rigid–flexible coupling dynamic model for the high-speed passenger car is then developed by using the FE and MBS coupling approach. The rail profiles in those particular locations are measured and further integrated into the simulation model to reproduce the bogie hunting and carbody elastic vibrations. The effects of wheel and rail wear on the vehicle system response, e.g. wheelset bifurcation graph and carbody vibrations, are studied. Two improvement measures, including the wheel profile modification and rail grinding, are proposed to provide possible solutions. It is found that the wheel–rail contact conicity can be lowered by decreasing wheel flange thickness or grinding rail corner, which is expected to improve the bogie hunting stability under worn rail and worn wheel conditions. The carbody elastic vibrations caused by bogie hunting instability can be further restrained.     

The effect of wheel set gyroscopic action on the hunting stability of high-speed trains

This study explores the effects of wheel set gyroscopic action on hunting stability by calculating linear and nonlinear critical speeds. First, a dynamic model for a high-speed vehicle with 23 degrees of freedom is developed by considering wheel set gyroscopic action. The linear and nonlinear critical speeds are calculated by eigenvalue analysis and drawing a bifurcation map, respectively. Two computer programs for linear and nonlinear stability analysis are developed. Second, based on an actual high-speed vehicle in China, the effects of wheel set gyroscopic action on hunting stability are quantitatively investigated using computer simulation. Furthermore, the difference between the effects of gyroscopic moments about the x-axis and z-axis is discussed. The results show that the moment about the x-axis is harmful to hunting stability, but the moment about the z-axis is beneficial to hunting stability. However, the integrated effect of these two moments can enhance the critical speeds and suppress the hunting motion.     

通过轮轨关系控制噪声

在城市轨道交通系统的运营环境中,都必然会产生噪声和振动,但这并不意味着乘客或沿线的工作人员和居民都能接受。     

高速铁路路基施工期沉降量的灰色预测模型及应用

为正确估算高速铁路无碴轨道路基施工期的沉降量,以京津客运专线工程为例,分别采用传统灰色预测模型和修正灰色预测模型对路基的沉降变形进行预测.研究结果表明:一方面,因为修正灰色预测模型对原始资料中已有信息的利用更加充分,所以,模型的精度更高;另一方面,修正灰色预测模型突破了传统灰色预测模型等间隔时序系统的限制,拓宽了模型在实际预测中的适用范围.因此.在实际应用中,推荐采用修正的非等时距灰色预测模型.     

基于建筑信息模型(BIM)的城市轨道交通设施设备分类与编码研究

城市轨道交通涉及多专业的设施设备,建设项目从设计、施工到运营维护,各阶段的设施设备信息采用不同的分类编码体系,难以开展设施设备的统一化管理。调研上海轨道交通既有的设施设备分类编码体系,基于建筑信息模型(BIM)技术研究,建立城市轨道交通全生命周期的设施设备分类编码体系。该分类编码体系是以城市轨道交通资产管理的分类编码为基础进行编制的,划分了21类,具有唯一性、稳定性、可扩展性等特点,可兼容既有的设施设备分类编码体系,并能够满足城市轨道交通运营维护管理的需求。     

铁路路基压实指标K30、Ev2、Evd对比分析

通过对铁路路基压实检测力学指标K30、Ev2、Evd之间检测原理的对比分析,表明K30、Ev2均反映了土的变形模量,是路基承载力大小的体现,是路基刚度大小参数,现场实测时建议以K30为主;Evd测试仪较静态变形模量Ev2各方面比较起来具有优势,已成为路基检测技术的发展方向。现场实测时,可作为首选检测指标,当Evd测试值检测合格后,再启用其他检测方法。     

陕西地区汉族群体Y染色体STR基因座多态性研究

目的　研究 14 0名陕西地区汉族男性个体Y染色体 6个STR基因座的遗传多态性 ,为法医学实践和群体学研究提供基础数据。方法　应用Y PLEXTM6荧光标记复合扩增系统 ,PCR扩增后用ABI 310 0Avant遗传分析仪进行基因扫描和基因分型。结果　 6个基因座分别检测出 4、6、7、8、4、37种等位基因 ,基因多样性 (GD)从 0 .35 94～0 .95 6 5。在 14 0名个体中共检测出 135种不同的单倍型 ,其中 131个只出现 1次 ,单倍型多样性 (HD)为 0 .992 3,个体识别能力 (DP)为 0 .96 4 3。结论　这 6个Y染色体STR基因座具有较好的多态性 ,联合单倍型具有较高的个体识别能力和非父排除率 ,适用于法医学实践和群体遗传学研究     

构建及鉴定携带角蛋白启动子和人乳头瘤病毒16E6/E7基因的重组腺病毒

目的利用AdEasy系统构建携带人角蛋白启动子的人乳头瘤病毒-16(HPV-16)E6/E7基因重组腺病毒,并通过RT-PCR方法检测E6/E7基因的表达。方法应用PCR方法从含有HPV-16全基因序列的质粒上扩增E6/E7基因,构建pCDNA3.1(-)-K14-E6/E7-polA载体,扩增、酶切获得K14-E6/E7-polA片段插入腺病毒穿梭载体质粒pAdTrack上,构建重组穿梭载体pAdTrack-K14-E6/E7-polA,线性化后与骨架载体AdEasy-1在细菌BJ5183内同源重组得到腺病毒质粒pAd-K14-E6/E7-polA,经人胚肾293细胞包装后得到重组腺病毒pAd-K14-E6/E7-polA。氢化铯(CsCl)梯度离心纯化病毒,提取病毒再感染后的293细胞总RNA,通过RT-PCR方法检测E6/E7基因的表达。结果通过同源重组的方法构建了腺病毒pAd-K14-E6/E7-polA载体,经酶切和测序鉴定该质粒构建成功。293细胞包装3d后观察到绿色荧光蛋白(GFP)表达,CsCl梯度离心纯化最终获得7.2×1010pfu/mL滴度的重组病毒;用该滴度病毒重新感染293细胞3d后,提取细胞总RNA,RT-PCR检测E6/E7有表达。结论利用新型腺病毒载体AdEasy系统可在短期内制备同时表达GFP和E6/E7的重组腺病毒pAd-K14-E6/E7-polA。这将为进一步研究HPV-16E6/E7基因功能及利用基因治疗女性宫颈癌奠定了基础。