改革开放30年，专用汽车与您同行

如果说乘用车与百姓生活密切相关的话,那么专用汽车则与国民经济发展息息相连。近10年来,专用汽车在我国国民经济中的地位越来越重要。三峡大坝、北京奥运工程、道路施工与清障、建筑运输、城市建设、医疗卫生、油田开发、抢险救灾等重点工程和行业,随处可见专用汽车忙碌的身影。     

根据IMO的导则进行航线设计

随着国际海事组织（IMO）及主要航运国家对船舶在海上安全的普遍重视，国际上出台了一系列航运法规。国际海事组织1999年11月25日出台的IMO．A．893（21）号决议是关于编制航线设计的导则，包括航次或航线设计的目的、评估、实施和监控五个部分，对船舶的航行安全，保护海上环境都提出了详细的准则。     

Preparation of recombinant firefly luciferase by a simple and rapid expression and purification method and its application in bacterial detection

A simple and rapid expression and purification method of recombinant firefly luciferase was developed for bacteria detection. A modified luciferase gene from North American firefly Photinus pyralis was cloned into pET28a expression vector and the recombinant protein was produced in Escherichia coli BL21. The recombinant luciferase, equipped with a polyhistidine affinity tag, was purified by immobilized metal ion affinity chromatography (IMAC). The approach generated an abundant expression and an efficient purification of a recombinant luciferase with final yield 1.995mg/L of cell culture. Experiments on the recombinant luciferase also showed that the relative light units (RUL) of the enzyme were 5.8×108, and the specific activity was 2.9×1010 RLU/mg. By applying adenosine triphosphate (ATP) bioluminescence to detection of the coin bacteria using the recombinant protein, the ATP content of bacteria was 9.48×10-16mol/mL, and was identical to the bacteria counts (4500CFU/mL) in order of magnitude. Taken together, our results provided a simple and efficacious method of the preparation of recombinant luciferase, which could be applied in the determination of bacteria via ATP bioluminescence.     

船载直升机着船预报系统及评估方法

介绍船载直升机着船预报系统的硬件组成和几种常用的预报方法,提出一种基于微成分分析的新的预报方法。最后,对综合考虑横摇和纵摇2种运动的着船预报效果进行评估。     

电控气缸注油器注油调频控制机理分析

为实现电控气缸注油器的注油调频控制,探究其注油调频的计算方法,提出注油调频的通用计算公式,分析该计算公式在研发电控气缸注油器注油调频机理上的适用方法。     

区间信号设备维修轨道车使用方式探讨

阐述温福客专铁路现行区间信号设备维修作业组织方式及所遇到的困难,通过比较说明使用轨道车进行区间信号设备检修作业的优越性;提出电务共用工务轨道车的3种方案并进行优劣性比较探讨。     

从习语看中西文化的差异

习语是语言的精华，它具有丰富的民族色彩，反映本民族的文化特征。本文从分析中英文习语人手，阐释了中西方在宗教信仰、经济生活、风俗习惯以及娱乐活动等方面的文化差异。     

集装箱租赁系统的研制

先进高效的集装箱租赁管理信息系统是租箱经营人开展集装箱租赁业务的重要组成部分,也是提高服务效率的技术保障.面对激烈的集装箱租赁市场竞争环境,国际上越来越多的租箱经营人加大信息系统建设的投入,以先进的信息系统提供优质高效的服务占据市场.东方国际租箱管理信息系统是中海信息研究团队独立研发的高科技成果,应用了多项创新实用技术,具有很好的示范性和推广价值.     

车辆碰撞中乘员骨盆力学响应特性的研究

本文旨在研究车辆碰撞中乘员骨盆损伤机理和年龄对碰撞响应的影响。首先开发并验证了老年人骨盆的有限元模型。其次,对比研究了3个具有不同年龄特征的骨盆模型(老年骨盆模型、中年骨盆模型GHBMC和儿童骨盆模型CHARM-10)在材料特性、生理特征、力学响应和损伤方面的差异。结果表明:具有相似生理几何尺寸的老年骨盆模型和中年骨盆模型与儿童骨盆模型相比有较大的差异。侧面碰撞实验的结果表明,3种年龄骨盆模型中,老年骨盆模型的碰撞峰值力和受力最大时位移量都最大,而碰撞中最大位移却最小。对碰撞响应数据进行拟合的结果表明,3种年龄骨盆模型的碰撞峰值力皆随冲击能量呈指数增长,而碰撞中最大位移量则随冲击能量呈线性增长。应力云图分析结果表明,骨盆在侧向冲击下最易受伤或骨折的部位是耻骨支、骶髂关节和髋臼。     

Systematic budget allocation for transportation construction projects: a case in Taiwan

It has always been a daunting task for any government to allocate the budgets for public infrastructure effectively so as to maximize the benefits of the limited resources. The most important contribution this paper makes is to conduct an empirical study of the budget allocations of the six region-based road system construction plans in northern Taiwan using the transportation budget allocation model devised in this paper. After reviewing the results, the experts consulted while preparing this paper believe that these results are both objective and able to meet the actual requirements. The competent authority also believes that they are better than those obtained using their original approach. In particular, the construction of this model takes into consideration the highest-level supervisors. To a certain degree, it caters for the practices in budget allocation so that the managers are able to assume the authority corresponding to their responsibility concerning management efficiency and final outcomes.