TGF-β_2对体外培养的牛眼小梁细胞MMP_2表达和活性的影响

目的了解转化生长因子-β2(TGF-β2)对体外培养的牛眼小梁(TM)细胞基质金属蛋白酶-2(MMP2)的表达和活性的影响,探讨TGF-β2在原发性开角型青光眼(POAG)发病机制中的作用。方法用含有1.0μg/L TGF-β2或含1.0μg/L TGF-β2+100g/L鼠抗人PAI-1IgG(PAI-1中和抗体)的无血清DMEM分别孵育TM细胞24、36、48h。对照组(Co)加入不含实验药物的无血清DMEM,用Western印迹法检测MMP2及纤溶酶原活化抑制剂-1(PAI-1)的表达。结果TGF-β2可显著增强TM细胞MMP2前体及PAI-1的表达。PAI-1中和抗体可促进MMP2前体向其活化形式转化。结论TGF-β2可通过抑制MMP2的活化过程导致TM细胞的细胞外基质(ECM)的异常堆积,造成房水引流阻力的增加,参与POAG的发病。     

肥胖高血压患者糖、脂质代谢紊乱的临床对照研究

目的观察肥胖高血压患者血脂、血糖、胰岛素指数及血清、脂肪组织中脂蛋白脂酶(LPL)含量和活性变化,探讨糖、脂质代谢紊乱与肥胖高血压及代谢综合征的发病关系。方法选取122例肥胖高血压患者及等量正常组病例进行对照研究,测定体质量指数(BMI)、腰臀围比(WHR)、血压、血脂、血糖、血胰岛素,并计算胰岛素敏感指数(ISI),部分病例测定血清及脂肪组织的LPL含量和活性。结果肥胖高血压组患者的血清甘油三脂(TG)、空腹血糖(FBS)、血胰岛素(INS)水平均显著高于对照组(P<0.01),而血清LPL含量、HDL水平、ISI显著低于对照组(P<0.01);两组间脂肪组织中LPL的含量无显著差别(P>0.05)。相关分析发现,血淸LPL含量与BMI(r=-0.64)、WHR(r=-0.59)、TG(r=-0.78)呈显著性负相关(P<0.01),与ISI(r=0.53)、HDL(r=0.46)呈正相关;脂肪组织中LPL含量及活性与上述指标均无相关性(P>0.05)。结论肥胖高血压患者多合并有糖、脂质代谢紊乱和胰岛素抵抗,血清LPL在其代谢紊乱中可能发挥重要作用。     

考虑拥挤的轨道交通网络时刻表协调优化建模

以非连通型城市轨道交通网络为研究对象,根据城市轨道交通网络周期性运行特点,在深入考虑拥挤及换乘客流脉冲性到达特征的前提下,给出1个循环周期内城市轨道交通网络运营费用及乘客出行费用计算方法.并在此基础上,以各运营线路发车间隔及发车时刻相位差为决策变量,以乘客及运营企业的综合费用最小为目标,以列车发车间隔、列车容量、站台容量、运营补贴等为约束,建立城市轨道交通网络列车时刻表优化模型.根据模型特点,提出了一种基于仿真的遗传算法对模型进行求解.算例结果显示,与既有优化方法相比,本文模型能够更加细致地刻画乘客换乘过程,有效降低系统综合费用,并确保各项服务指标在安全范围之内.     

TDOA Passive Location Based on Cuckoo Search Algorithm

This paper formulates a new framework to estimate the target position by adopting cuckoo search(CS)positioning algorithm. Addressing the nonlinear optimization problem is a crucial spot in the location system of time difference of arrival(TDOA). With the application of the Levy flight mechanism, the preferential selection mechanism and the elimination mechanism, the proposed approach prevents positioning results from falling into local optimum. These intelligent mechanisms are useful to ensure the population diversity and improve the convergence speed. Simulation results demonstrate that the cuckoo localization algorithm has higher locating precision and better performance than the conventional methods. Compared with particle swarm optimization(PSO) algorithm and Newton iteration algorithm, the proposed method can obtain the Cram′er-Rao lower bound(CRLB) and quickly achieve the global optimal solutions.     

Catalytic Combustion of Lean Methane Assisted by Electric Field over Pd/Co<Subscript>3</Subscript>O<Subscript>4</Subscript> Catalysts at Low Temperature

A series of Pd/Co₃O₄ catalysts were prepared by Self-Propagating High-Temperature Synthesis (SHS) method in this study, and electric field was applied for catalytic combustion of lean methane over Pd/Co₃O₄ catalysts at low temperature. When electric field was applied, the catalytic combustion performance of Pd/Co₃O₄ catalysts was greatly improved, and the application of electric field could reduce the load of active element Pd to some extent while maintaining the same efficiency. Based on experimental tests and the analysis results of X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), H₂-temperature-programmed reduction (H₂-TPR) and in-situ diffuse reflectance infrared Fourier transform spectroscopy (in-situ DRIFTS), the mechanism of catalytic oxidation of CH₄ over Pd/Co₃O₄ catalysts in electric field was proposed. The catalytic combustion of CH₄ occurs only when the temperature is higher than 250 °C normally, but when electric field was applied, the whole process of CH₄ oxidation was promoted significantly and the reaction temperature was reduced. Electric field could promote the reduction of the support Co₃O₄ to release the lattice oxygen, resulting in the increase of PdO_x and the surface chemisorbed oxygen, which could provide more active sites for the low-temperature oxidation of CH₄. Furthermore, electric field could accelerate the dehydroxylation of CoOOH to further enhance the activity of the catalysts.     

公交集团保持党员队伍适度规模和提高党员队伍质量问题探讨

党员数量是党的基础,党员质量是党的根本。如何保持党员队伍适度规模和提高党员队伍质量,发挥其先锋模范引领带动作用,是公交企业当前和今后的重要工作。近年来,乌鲁木齐公交集团党委以“党要管党,从严治党”为引领,立足公交实际,实行发展党员总量调控,使集团党员队伍保持适度规模;根据公交特点,侧重生产一线发展党员,不断优化党员队伍结构。     

设计以人为本的街道——以东莞市东城世博商业圈道路设计为例

中国很多城市道路往往以机动车交通为主要设计对象,而忽视步行和自行车交通.阐述当前世界各大城市和地区以人为本的街道设计理念和趋势,总结国外街道设计基本原则,并辅以国际经验示例.以东莞市东城世博商业圈道路设计为例,阐述商业区、住宅区等人流量较大的区域进行人性化道路设计的要点.通过压缩机动车道数、设计中间分隔带、设置非机动车道、统筹布置设施带等方法,设计更加人性化的道路横断面;通过抬升交叉口、采用较小路缘石半径、交通稳静化措施等方法,设计更加人性化的交叉口.指出以人为本的街道设计有利于创建富有活力、可持续发展的宜居城市.     

伽玛刀治疗颅脑疾病253例报告

目的　探讨OUR XGD旋转式伽玛刀对颅脑疾病的治疗效果。方法　回顾性分析了从1 999年 6月至 2 0 0 0年 7月应用OUR XGD旋转式伽玛刀治疗颅脑疾病 2 5 3例的临床疗效。结果　临床随访 1 6 7例 ,影像学随访 1 0 5例 ,随访时间为 1～ 1 2个月。治疗有效率在 1 5 5例颅内占位性疾病为 93 % ,1 2例功能性疾病达 83 3 %。结论　旋转式伽玛刀是治疗颅内疾病的有效方法。     

Development of Thermal Process and Numerical Modeling

IntroductionWithinrecentdecade,thenumericaIsimulationhasbeenrapidlyachievedandhasaPpliedtothermalmanufacturingprocess.ThephasetransformationiscomplicatedbythefactthatthereareseveralmicrostructuralscaIestoconsideHl1-I4].InordertorevealtheruIeofcomplexphasetransformation,itisnecessarytousethetoolofnumericalsimulationtomodelandcontroltheoverallprocessofphasetransformation.NowthecomputersimulationcangetsomereasonabIeresultSinmanyaspectsofthermalmanufacturingprocess:e.g.,thesimulationoftemperat…     

常张高速公路粉喷桩质量检测与评价

常张高速公路是湖南省内首例大规模采用粉喷桩进行软土地基加固的公路工程。结合其工程实际,参照《公路质量检测评定标准》中的各项指标,制定了一套粉喷桩综合检测程序及其质量评价方法。