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31.
Desjardins Elise Higgins Christopher D. Scott Darren M. Apatu Emma Páez Antonio 《Transportation》2022,49(3):867-895
Transportation - Bicycling is an increasingly popular mode of travel in Canadian urban areas, like the Greater Toronto and Hamilton Area (GTHA). While trip origins and destinations can be inferred... 相似文献
32.
Bacterial pore-forming toxins (PFTs) are essential virulence factors of many human pathogens. Knowl- edge of their structure within the membrane is critical for an understanding of their function in pathogenesis and for the development of useful therapy. Atomic force microscopy (AFM) has often been employed to structurally interrogate many membrane proteins, including PFTs, owing to its ability to produce sub-nanometer resolution images of samples under aqueous solution. However, an absolute prerequisite for AFM studies is that the samples are single-layered and closely-packed, which is frequently challenging with PFTs. Here, using the prototypical member of the cholesterol-dependent cytolysin family of PFTs, perfringolysin O (PFO), as a test sample, we have developed a simple, highly robust method that routinely produces clean, closely-packed samples across the entire specimen surface. In this approach, we first use a small Teflon well to prepare the supported lipid bilayer, remove the sample from the well, and then directly apply the proteins to the bilayer. For reasons that are not clear, bilayer preparation in the Teflon well is essential. We anticipate that this simple method will prove widely useful for the preparation of similar samples, and thereby enable AFM imaging of the greatest range of bacterial PFTs to the highest possible resolution. 相似文献
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阐述甘泉铁路车站分布和运量发展情况及货运量增加后区间通过能力不足的问题,介绍甘泉铁路行车组织和区间通过能力计算方法,计算各区间的通过能力,结合近期货物运量500万t、700万t、1000万t三种情况,计算需求通过能力,并给出提高通过能力的具体方案,为下一步货运量的增加做准备。 相似文献
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使用有限元分析以改善重载运输车轴 总被引:1,自引:0,他引:1
分析了北美铁路货运发生的车轴故障,介绍了使用有限元对F级和K级车轴进行应力分析计算,研究车轴尺寸变化对应力的影响,并提出了改善重载车轴设计的建议. 相似文献
37.
Upon the expiry of the concession contract signed between Lebanon and a private company for the Port of Beirut operation (in December 1990), the Government appointed a temporary commission to operate the port under the rules of the expiring concession. The legality of this measure has since always been questioned, while the temporary commission underwent three major personnel changes which clearly prevented any long-term thinking or planning. In 1998, the temporary commission entered into a 20-year joint venture for the establishment of a container terminal. Meanwhile, Lebanon's two main ports were facing major regional competition, while competing against each other to some degree. Thus, the need became urgent to end the temporary status and propose reforms to the entire sector. To that end, the Ministry of Public Works and Transport was commissed by the Government to propose a permanent institutional framework for the port of Beirut while assessing the need for a national maritime sector regulator. This paper presents the recommendations for the institutional reform of the Lebanese maritime transport sector. 相似文献
38.
A time-domain technique for estimating wave-induced ship hull bending moment from ship motion measurements is presented. This technique is developed to improve hull response monitoring systems. Artificial neural networks are used to model the time-domain relationship between the wave-induced vertical bending moment and the coupled heave and pitch motions. The application of the proposed technique is validated using experimental data. 相似文献
39.
Analysis of factors that may be essential in the decision to fly on fully autonomous passenger airliners
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This research investigates factors that influence opinion in the decision to fly on fully autonomous passenger airliners primarily from the perspective of aviation and technology professionals. Bayesian statistical inference and a two‐level fractional factorial survey are used to sample passengers' views on fully autonomous airliners. Eight trust, safety, and cost factors are incorporated into a vignette set in the future. Factors include automation levels, safety records, liability guarantees, airline integrity, and service disruptions. Dependent variables exist in five post‐vignette questions and essentially ask “Would you” or “Would you not” be willing to fly on a fully autonomous airliner? Sixteen versions of the vignette, each with unique trust, safety, and cost levels, present varying (unknown) degrees of influence to the survey respondents. For every demographic, the research shows a 99% statistically significant difference between the “prior” and “posterior” sampled population proportions willing to fly. The most significant positive influence involves integrity characteristics of the airline, while the most negative influence relates to life insurance liability guarantees. Research from 2003 suggested that this mode of travel would be acceptable to only 10.5% of respondents. When the 2003 research is used as a Bayesian prior probability, the resulting posterior probability for the demographics sampled can be modeled as a beta distribution, indicating 95% probability that the sampled proportion of the population willing to fly is between 33.2% and 36.4%. After adjusting for age and profession demographics to match the US population, the 95% probability bounds on the proportion willing to fly are 31.35% and 34.15%. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
40.
In this paper, three innovative car-sharing systems for urban areas are proposed, based on fleets of individual intelligent vehicles with three service characteristics: instant access, open-ended reservations and one-way trips. These features provide high flexibility but create an uneven distribution of vehicles among stations. Therefore, relocation of vehicles must be performed. Three different system procedures are proposed: in the first system, relocations are performed by users; in the other two, vehicles relocate automatically, thanks to their automation. In the first two systems, vehicles are accessible only at stations, whereas in the third they are also accessible along roads. In order to provide transport managers with a tool to test systems in different realities, an object-oriented simulator is developed. The simulation provides outputs of system performance, in terms of user waiting times and system efficiency. The proposed systems are simulated for the city of Genoa, in Italy, and a comparative analysis is presented. 相似文献