袋包装船机及其螺旋溜槽的研究与应用

针对沿海、沿江袋装货物的专业化装卸需要设计了一种袋包装船机,论述了其装卸工艺与结构型式,建立了刚性滑槽和柔性袋包的刚柔耦合动力学模型,仿真确定了袋包装船机的核心部件螺旋滑槽的几何参数,应用结果表明所设计的袋包装船机能够满足设计要求。     

机桨联合控制方法研究

介绍了机桨联合控制的基本思想和原理,分析了机桨联合控制的关键问题,针对机桨联合控制的交叉耦合问题,提出了通过预测控制实现机桨联合控制的方法,结论表明该方法是有效的。     

A novel approach to design robust fault detection filter for networked control systems

In this paper, an approach for designing robust fault detection filter (RFDF) of networked control systems (NCSs) with unknown inputs is studied. The design aims at implementing the optimal trade-off between robustness of unknown inputs (including the item produced by networked-induced delay) and sensitivity of fault. The key design issue is to introduce an optimal fault detection filter based on NCSs with the control law compensation as the reference residual model of NCSs and to formulate the RFDF design as a model-matching problem. By applying H _∞ optimization technique, linear matrix inequality (LMI) approach is given to solve the model-matching problem. The validity of the proposed approach is shown by a numerical example.      

Surface reconstruction of scanned human body using radial basis functions and adaptive partition of unity

It is important to reconstruct a continuous surface representation of the point cloud scanned from a human body. In this paper a new implicit surface method is proposed to reconstruct the human body surface from the points based on the combination of radial basis functions (RBFs) and adaptive partition of unity (PoU). The whole 3D domain of the scanned human body is firstly subdivided into a set of overlapping subdomains based on the improved octrees. The smooth local surfaces are then computed in the subdomains based on RBFs. And finally the global human body surface is reconstructed by blending the local surfaces with the adaptive PoU functions. This method is robust for the surface reconstruction of the scanned human body even with large or non-uniform point cloud which has a sharp density variation. Foundation item: the National Natural Science Foundation of China (No. 50575139) and the Shanghai Special Fund of Informatization (No. 088)     

橡胶沥青嵌入式封层施工工艺研究

本文对橡胶沥青嵌入式封层的施工技术进行了研究。采用橡胶沥青嵌入式封层,可以提高道路的使用功能,提高道路的稳定性耐久性并且降低工程造价,缩短建设工期。此工艺优于其他养护方法,特别是3 cm沥青混凝土罩面。     

Assessing a soft twin tunneling numerical model using field data

Using a five-floor building affected by the Yangtze River highway tunnels in Wuhan as the engineering background, we have constructed a free-field model and a coupled model to study the soil, lining, foundations and upper structure, and analyze the rules of movements of building foundation and ground induced by single tunnel and twin tunnel excavation with the Finite Element Analysis method. It is shown that for the coupled model, the longitudinal displacement of each foundation increases slowly when the tunnel face gets close to the foundation section and then increases fast when the tunnel face moves away from the foundation during the single and twin tunneling. For a single tunnel, the surface settlements are overestimated by the free-field and coupled tunnel. This might be crucial in urban areas. Regarding the maximum settlements and the width of the settlement trough, the difference between the free-field model and the coupled model is quite obvious. This comparison with the field measurement value reveals that the coupled model seems to be superior to the free-field model. These results are of instructive significance for design and excavation.     

Cloning and sequencing of a DNA fragment encoding N37 apoptotic peptide derived from p53

Objective It was reported that p53 apoptotic peptide (N37) could inhibit p73 gene through being bound with iASPP, which could induce tumor cell apoptosis. To further explore the function of N37, we constructed the cloning plasmid of DNA fragment encoding p53 (N37) apoptotic peptide by using DNA synthesis and molecular biology methods. Methods According to human p53 sequence from the GenBank database, the primer of p53(N37) gene was designed using Primer V7.0 software. The DNA fragment encoding p53 (N37) apoptotic peptide was amplified by using self-complementation polymerase chain reaction (PCR) method and cloned into the pGEM-T Easy vector. The constructed plasmid was confirmed by endonuclease analysis and sequencing. Results The insertion of objective DNA fragment was confirmed by plasmid DNA enzyme spectrum analysis, p53 (N37) gene was successfully synthesized chemically in vitro. The sequencing result of positive clone was completely identical to the human p53(N37) sequence in GenBank using BLAST software (http://www. ncbi. him. nih. gov/cgi-bin /BLASTn). Conclusion The cloning of DNA fragment encoding p53(N37) apoptotic peptide was constructed by using DNA synthesis and pGEM-T Easy cloning methods. With the constructed plasmid, we could further investigate the function of N37 peptide.     

Effect of ulinastatin on hepatic ischemia-reperfusion injury in rats

Objective To investigate the effect of ulinastatin (UTI) on hepatic ischemia-reperfusion injury in rats. Methods Totally 24 adult Sprague-Dawley rats were randomly divided into 3 groups: sham-operated control group (SO group), ischemia-reperfusion group (I/R group) and ulinastatin group (UTI group). Liver in I/R group underwent 1 h of reperfasion after 30 min of ischemia. In UTI group, UTI (2×104 U/kg) was administered to rats 30 min before modeling. The levels of alanine aminotransferase, aspartate aminotransferase and tumor necrosis factor-alpha (TNF-α) in serum were measured and the levels of nitric oxide and malondialdehyde in liver were determined. The histological changes of liver were observed. Results The levels of alanine aminotransferase, aspartate aminotransferase and TNF-α in serum were significantly increased in I/R group compared with those in UTI group (P<0.05). The levels of nitric oxide and malondialdehyde in liver were significantly higher in I/R group than in UTI group (P<0.05).Histological examination of liver indicated that the damages were more severe in I/R group than in UTI group.Conclusion UTI has the ability to inhibit the production of TNF-α and oxyradical, and ameliorate microcirculatory dysfunction in rats with hepatic ischemia-reperfasion injury.     

Screening of hepatocyte proteins binding to NS5ABP37 protein by yeast-two hybrid system

Objective To investigate the biological function of NS5ABP37 and to look for proteins interacting with NS5ABP37 protein in hepatocytes. Methods We constructed bait plasmid expressing NS5ABP37 protein of hepatitis C virus (HCV) by cloning the gene of NS5ABP37 protein into pGBKT7, then the recombinant plasmid DNA was transformed into yeast AH109 (α type). The transformed yeast AH109 was mated with yeast Y187 (α type) containing liver cDNA library plasmid in 2×YPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium (SD/-Trp-Leu-His-Ade) containing X-α-gal for selection and screening. After extracting and sequencing of plasmids from positive (blue) colonies, we made a sequence analysis by bioinformatics. Results We screened twenty-five proteins binding to NS5ABP37, including Homo sapiens cyclin Ⅰ (CCNI) gene, Homo sapiens matrix metallopeptidase 25 (MMP25) and Homo sapiens talin 1. Conclusion The yeast-two hybrid system is an effective method for identifying hepatocyte proteins interacting with NS5ABP37 of HCV. And the biological function of NS5ABP37 may be associated with glycometabolism, lipid metabolism and apoptosis.     

Screening of the effective cellulose-degradable strain and its application in the production of cellulose bioethanol

Strains from the cellulose-containing environment were collected. Primary screening(by filter-paper Hutchison solid culture medium and sodium carboxymethylcellulose solid culture medium) and reelection(by filter-paper inorganic salt culture medium and sodium carboxymethylcellulosc Congo red coltnre medium) indicated that five strains obtained were best suited for high performance cellulose degradation. Determination of sodium carboxymethylcellulose activity(CMCA) and filter paper activity(FPA) was accomplished for each of the five. The strongest of the five in CMCA and FPA was applied to the production of cellulose bioethanol by separate hydrolysis and fermentation(SHF) and simultaneous saccharification and fermentation(SSF) respectively.