船舶登记审查形式的探讨

船舶登记就是对船舶享有某种权利的人，向国家授权的船舶登记机关提出申请并提交相应的文件，经船舶登记机关审查，对符合法定条件的予以注册，并以国家的名义签发相应证书的法律事实。     

GPS RTK技术在铁路客运专线放线中的应用

以Trimble 4700（或5700）GPS接收机为例，介绍了GPS RTK在铁路客运专线放线中的应用过程，重点叙述了坐标转换参数的求解和设置。通过GPS RTK放线试验阐述了GPS RTK在铁路客运专线放线中的可行性及优势，并对铁路客运专线的放线工作提出了建议。     

站场形数据结构设计研究

站场形数据结构是微机联锁软件系统的重要组成部分。文中介绍了站场形数据结构的各组成要素，举例说明其设计原理，最后指出了构造站场形数据结构所必须遵循的原则。     

施工企业提高中标率的有效途径

通过对施工企业面临的投标形势及投标情况的分析，阐述了企业提高中标率的有效途径。     

A capacity restraint transit assignment with elastic line frequency

This paper proposes a new formulation for the capacity restraint transit assignment problem with elastic line frequency, in which the line frequency is related to the passenger flows on transit lines. A stochastic user equilibrium transit assignment model with congestion and elastic line frequency is proposed and the equivalent mathematical programming problem is also formulated. Since the passenger waiting time and the line capacity are dependent on the line frequency, a fixed point problem with respect to the line frequency is devised accordingly. The existence of the fixed point problem has been proved. A solution algorithm for the proposed model is presented. Finally, a numerical example is used to illustrate the application of the proposed model and solution algorithm.     

多房棘球绦虫重组Bb-Em14-3-3疫苗的构建及其表达效率

目的构建多房棘球绦虫(Em)重组双歧杆菌(Bb)-Em14-3-3疫苗,并研究Em14-3-3分子在大肠埃希菌BL21(DE3)中的表达效率。方法通过PCR扩增Em14-3-3抗原编码基因;将该基因定向克隆于含有谷胱甘肽-S-转移酶(GST)基因的大肠埃希菌-双歧杆菌穿梭表达载体pGEX-1λT,构建重组质粒pGEX-Em14-3-3;用电穿孔法将该质粒导入Bb,构建多房棘球绦虫重组Bb-Em14-3-3疫苗。经PCR和酶切鉴定后以IPTG诱导表达Em14-3-3/GST融合蛋白;SDS-PAGE及Western blot对表达产物进行鉴定。结果PCR成功扩增出530 bp的Em14-3-3基因;双酶切证实Em14-3-3基因插入pGEX-1λT中;PCR证实重组Bb-Em14-3-3疫苗构建成功;SDS-PAGE及Western blot分析显示重组质粒转化宿主菌在IPTG诱导下高效表达了Em14-3-3/GST融合蛋白,表达效率为23%。结论成功构建了多房棘球绦虫重组Bb-Em14-3-3疫苗,重组质粒pGEX-Em14-3-3在大肠杆菌中获得了高效表达,Western blot结果提示表达出的Em14-3-3重组蛋白具有特异抗原性。     

基于人工神经网络预测的汽车安全气囊系统点火控制算法研究

对安全气囊点火时刻的准确控制是气囊系统起到保护乘员作用的关键技术.点火控制算法是实现气囊适时准确点火的手段.本文提出了一种基于人工神经网络的汽车安全气囊点火控制算法.算法构造了一个三层人工神经网络,利用碰撞发生时的减速度信号和乘员重量为输入信号,其输出信号为乘员的头部位移,作为气囊触发的一个依据.     

边坡防护室内水力冲刷模拟试验及降雨入渗分析

阐述了边坡防护室内水力冲刷模拟试验的目的、方法和步骤.针对不同边坡防护型式、不同坡率、不同土质状况的五种试件进行了降雨入渗试验,对结果进行了分析,并总结出不同工况下的降雨入渗规律.     

基于可持续发展的城市交通微循环路网优化模型

文章基于可持续发展的理念,建立了交通微循环的路网优化双层模型：上层为考虑支路改扩建和新建的混合网络设计问题,下层为符合UE准则的运量分布和平衡配流组合模型;提出遗传算法对模型进行求解。模型对交通微循环项目的实施建设有一定指导意义。     

An interpretation of inter-container port relationships from the demand perspective

Container ports serve as important conduits to facilitate the efficient flow of containerized cargo. As part of value-driven chain systems that intersect between hinterlands, efficiency gains that are generated within the container port will have a direct impact on the competitive advantage of its users and affect the economic potential of both the origin and destination hinterlands. As such, the paper proposes the usage of indifference analysis propounded in microeconomic theory as a useful means to examine inter-container port competition and complementarity. The framework presented can also be combined with Porter's 'Diamond' model in order for inter-container port demand relationships to be quantified, measured and analysed in an holistic manner. Apart from analysing inter-port relationships from the standpoint of container ports, the framework can also be used to analyse inter-port relationships from the perspective of other key players in the value-driven chain system and modified accordingly to incorporate various indicators that are deemed to be relevant to these parties.