Novel experimental strategy for high resolution AFM imaging of membrane-associated bacterial toxins

Bacterial pore-forming toxins （PFTs） are essential virulence factors of many human pathogens. Knowl- edge of their structure within the membrane is critical for an understanding of their function in pathogenesis and for the development of useful therapy. Atomic force microscopy （AFM） has often been employed to structurally interrogate many membrane proteins, including PFTs, owing to its ability to produce sub-nanometer resolution images of samples under aqueous solution. However, an absolute prerequisite for AFM studies is that the samples are single-layered and closely-packed, which is frequently challenging with PFTs. Here, using the prototypical member of the cholesterol-dependent cytolysin family of PFTs, perfringolysin O （PFO）, as a test sample, we have developed a simple, highly robust method that routinely produces clean, closely-packed samples across the entire specimen surface. In this approach, we first use a small Teflon well to prepare the supported lipid bilayer, remove the sample from the well, and then directly apply the proteins to the bilayer. For reasons that are not clear, bilayer preparation in the Teflon well is essential. We anticipate that this simple method will prove widely useful for the preparation of similar samples, and thereby enable AFM imaging of the greatest range of bacterial PFTs to the highest possible resolution.     

Plate scanning tools to obtain travel times in traffic networks

The management of vehicle travel times has been shown to be fundamental to traffic network analysis. To collect travel time measurement, some methods focus solely on isolated links or highway segments, and where two measurement points, at the beginning and at the end of a section, are deemed sufficient to evaluate users' travel time. However, in many cases, transport studies involve networks in which the problem is more complex. This article takes advantage of the plate scanning technique to propose an algorithm that minimizes the required number of registering devices and their location in order to identify vehicles candidates to compute the travel times of a given set of routes (or subroutes). The merits of the proposed method are explained using simple examples and are illustrated by its application to the real network of Ciudad Real.     

Nanoparticle emission characteristics and reduction strategies by boost pressure control and injection strategies in a passenger diesel engine

This research attempted to analyze nanoparticles and other harmful exhaust emissions in accordance with injection strategies and air-fuel ratio (AFR) changes for small diesel engines. The emission characteristics were analyzed in the medium-speed condition, which is the main driving range of a diesel engine. In the case of particulate matter (PM), the number of particles was measured, analyzed, and compared to identify the correlation and emission characteristics of nanoparticles by using a dilution device and condensation particle counter (CPC), which are international standards for particle measurement recommended by the Particulate Measurement Programme (PMP). The engine torque tended to be reduced as pilot injections were added, and the torque was increased by the increased boost pressure, but reduced by the exhaust pressure increase in a part of the low-load range. The number of nanoparticles was not influenced greatly by the change in AFR, but the reduction effect on the PM weight was great depending on the boost pressure increase. In addition, the number of nanoparticles tended to increase as the fuel injection timing became closer to TDC in all conditions, and its difference became larger with an increase in AFR. In addition, in the case of the pilot injection, nanoparticle emission showed similar characteristics depending on the main injection timing, but it was increased by advanced injection timing when performing the main injection only, and the number of the nanoparticles increased as pilot injections were added. Last, the optimal conditions for EMS calibration were analyzed by selecting the conditions of torque reduction and NOx increase within 5 % from all of the engine operating conditions; optimized conditions are presented.     

Vibration reduction of H/Shaft using an electromagnetic damper with mode change

The objective of this study is to develop a damper that can reduce the amplitude of vibration in various frequency ranges. Previous H/Shaft vibration reduction methods work in a passive way. A dynamic damper reduces the amplitude of vibration at its first mode, but vibration still appears at the second mode. A mass damper or hollow shaft can shift the natural frequency to a lower or higher region. The fixed operating frequency prevents vibration from being reduced outside the operating frequency range. The proposed damper uses electromagnets as either masses or actuators to change the damper mode between dynamic damper mode and mass damper mode. The electromagnetic damper (EMD) can change its mode to respond to the vibration excitation at both low and high frequencies. The vibration reduction performance was evaluated by FRF tests in laboratory and vehicle conditions. The results were compared with those of a dynamic damper and indicate that the amplitude of vibration is reduced by 95.6 % when the EMD is implemented on an H/Shaft, whereas only 61.9 % vibration reduction is achieved by the dynamic damper.     

A study on the stability of a motorcycle wheel–swingarm suspension with chain transmission

The present study describes a possible driving mechanism for a self-excited oscillation observed in motorcycle dynamics, often referred to as chatter. This phenomenon, affecting the performance of road racing motorcycles, has been simulated in straight running braking manoeuvres with multibody motorcycle models. It involves rear suspension bounce and driveline oscillation in the frequency range between 17 and 22?Hz. A simplified model of a motorcycle rear suspension with chain transmission is proposed and its stability in equilibrium configurations is studied via eigenvalue analysis. The sensitivity with respect to all its governing parameters is analysed by means of stability maps and the self-excitation mechanism is explained with the aid of energy balance analysis and phase diagrams. It is found that the key role for the instability onset is played by the gradient of the nonlinear characteristic slip function of the tyre.     

A receding horizon sliding control approach for electric powertrains with backlash and flexible half-shafts

Active control of electric powertrains is challenging, due to the fact that backlash and structural flexibility in transmission components can cause severe performance degradation or even instability of the control system. Furthermore, high impact forces in transmissions reduce driving comfort and possibly lead to damage of the mechanical elements in contact. In this paper, a nonlinear electric powertrain is modelled as a piecewise affine (PWA) system. The novel receding horizon sliding control (RHSC) idea is extended to constrained PWA systems and utilised to systematically address the active control problem for electric powertrains. Simulations are conducted in Matlab/Simulink in conjunction with the high fidelity Carsim software. RHSC shows superior jerk suppression and target wheel speed tracking performance as well as reduced computational cost over classical model predictive control (MPC). This indicates the newly proposed RHSC is an effective method to address the active control problem for electric powertrains.     

Insights into vehicle trajectories at the handling limits: analysing open data from race car drivers

Race car drivers can offer insights into vehicle control during extreme manoeuvres; however, little data from race teams is publicly available for analysis. The Revs Program at Stanford has built a collection of vehicle dynamics data acquired from vintage race cars during live racing events with the intent of making this database publicly available for future analysis. This paper discusses the data acquisition, post-processing, and storage methods used to generate the database. An analysis of available data quantifies the repeatability of professional race car driver performance by examining the statistical dispersion of their driven paths. Certain map features, such as sections with high path curvature, consistently corresponded to local minima in path dispersion, quantifying the qualitative concept that drivers anchor their racing lines at specific locations around the track. A case study explores how two professional drivers employ distinct driving styles to achieve similar lap times, supporting the idea that driving at the limits allows a family of solutions in terms of paths and speed that can be adapted based on specific spatial, temporal, or other constraints and objectives.     

自行车流视频识别判断方法研究

考虑算法的实时性,交通流个体识别往往采用轮廓特征来描述。由于自行车与行人的很多轮廓特征值相近,在复杂城市交通环境中,从慢行交通流中准确地识别出自行车是目前采用视频检测交通流的难点之一。通过现场视频检测获取足够的样本外轮廓数据,分析检测目标轮廓的高、宽、高宽比、面积等典型轮廓特征,发现目标轮廓高宽比特征能较好地识别出行人、自行车等慢行交通,并给出合适的取值范围。研究表明自行车高宽比能够较好地成为视频识别的依据,为自行车视频识别提供数据。     

甘泉铁路区间通过能力研究

阐述甘泉铁路车站分布和运量发展情况及货运量增加后区间通过能力不足的问题,介绍甘泉铁路行车组织和区间通过能力计算方法,计算各区间的通过能力,结合近期货物运量500万t、700万t、1000万t三种情况,计算需求通过能力,并给出提高通过能力的具体方案,为下一步货运量的增加做准备。