Oleanolic acid-induced apoptosis and its relation with intracellular calcium in human lung adenocarcinoma A549 cells

Objective To investigate the effect of oleanolic acid (OA) on apoptosis, correlation between apoptosis and intracellular calcium, and its mechanism in human lung adenocarcinoma cell line A549. Methods Human lung adenocarcinoma A549 cells were incubated in vitro and assigned with OA concentrations of 0, 10, 20 and 40μg/mL. The apoptosis status of A549 cell line was detected with Annexin V-FITC/PI by flow cytometry (FCM); fluorescence intensity (FI) of A549 cells was assessed and the level of intracellular calcium was calculated at 24 hour of OA intervention. The relation between apoptosis and calcium FI was illustrated by curve fitting. Results FCM showed that 10, 20 and 40μg/mL of OA could induce A549 cell apoptosis, which followed a concentration-effect pattern; 24-hour intervention with 20μg/mL and 40μg/mL OA showed increased A549 cell apoptosis, and was significantly different from that with 0μg/mL OA (P<0.01). The FI of intracellular calcium concentration in 10, 20 and 40μg/mL OA groups was significantly higher than that in 0μg/mL group after 24 hours' intervention, and the FI showed a trend of increase with increased OA concentration (P<0.01). Curve fitting showed a significant correlation between apoptosis rate and intracellular calcium concentration in A549 cells (r=0.981, P<0.01). Regression equation was Y=0.508X-1.627. Conclusion OA plays a role in inducing apoptosis of human lung adenocarcinoma cells in a concentration-dependent manner. The OA-induced apoptosis is responsible for intracellular calcium overload of the tumor.     

Report on 220 cases of endoscopic septoplasty with submucous resection

Objective To explore the efficiency of endoscopic septoplasty with submucous resection for the treatment of 220 patients with deviated nasal septum and report our experience in this field. Methods From May 2006 to May 2010, 220 patients with deviated nasal septum were treated by endoscopic septoplasty with submucous resection to decompress the stress points, resect the deviated parts and implant the reshaped bone of nasal septum, while retaining the normal structure of nasal septum with minimally invasive technique. Results Satisfactory effects were achieved in all the 220 patients, showing centered septal structure and disappeared symptoms such as nasal obstruction, headache and nasal hemorrhage, but no complications including perforation of nasal septum, depressed nasal bridge and septal chalasia and flaring. Conclusion Rhino-endoscopic septoplasty with submucous resection can substantially retain the original structure of nasal septum and thus is proven to be a desirable operation with multiple advantages including minimal invasiveness, quicker healing and fewer complications.     

宁东地区煤矸石应用于道路基层材料的可行性研究

探讨了宁东地区煤矸石用于道路基层的可行性,设计了水泥粉煤灰稳定煤矸石基层材料。认为宁东地区的煤矸石,经过合理设计与试验验证之后应用于道路基层是可行的,并将产生较大环境效益和经济效益。     

燃油硫含量对国Ⅳ柴油轿车颗粒物排放特性的影响

对燃用硫含量分别为300mg/kg与43mg/kg的柴油和是否安装DPF对采用典型国Ⅳ排放控制技术的柴油轿车颗粒物排放特性的影响进行了试验研究.结果表明,未装DPF时,国Ⅳ柴油车燃用高含硫量燃油时的颗粒物质量排放较燃用低含硫量燃油时增加25.3%;安装DPF时增加22.2%.而颗粒物数量排放结果说明,燃油含硫量对安装DPF车辆的颗粒物数量浓度影响较大,燃用高含硫量燃油时的循环平均颗粒物数量浓度约为燃用低含硫量燃油时的4.8倍.研究同时表明,颗粒物排放主要在加速阶段产生,稳态工况和减速下颗粒物数量排放大幅降低.     

A Study on the Correlation Between VSP and Vehicle Emission Based on On-board Emission Test

搭建车载排放测试试验平台,选择轻型车和中型车进行了实际道路排放测试,建立了排放数据与行驶工况数据对应的数据库.引入比功率的概念比较了机动车排放随比功率和速度的变化关系,分析了轻型车和中型车比功率的分布频率和3种污染物的排放随比功率的变化关系.结果表明,轻型车和中型车比功率集中在-3～7kW/t范围;和速度相比,机动车的排放与比功率有更强的相关性.     

直道钢桥面沥青铺装层动应变测试与分析

采用动态数据采集系统进行钢桥面沥青混合料铺装层动应变的测试 ,分析了车速和温度对动应变的影响。     

无锡市城市防洪建设与城市环境建设的实践反思

本文论述了城市防洪建设与城市环境建设两者之间的密切关系 ;介绍了无锡市在处理这两者关系时的一些做法。     

主跨348m+348m三塔单索面PC梁斜拉桥

宜昌市夷陵长江大桥采用三塔斜拉桥，总体布置为（128．08＋348＋348＋120．35）m。介绍该桥的合理性及结构特点。     

汽车传动系扭振特征辨识

本文以SP141客车为例,运用特征系统“实现”算法研究了传动系扭振特征。文中主要论及:扭振系统特征辨识的数学原理;扭振模态试验技术,并给出应变模态与运动模态间的转换关系;汽车传动系统的扭振特征。     

提高路堤下软基土工织物加筋效果的综合措施

提出了多种工程措施 ,以提高路堤下软基土工织物的加筋效果 ,这些措施是 :( 1 )选择堤底宽度较小的工程 ,避开加筋垫层尺寸效应的不利影响 ;( 2 )采用预应变加筋法 ;( 3)堤身填土要有一定的塑性 ,避免脆性破坏 ;( 4 )采用应变法确定织物的允许抗拉强度。这些措施在工程中极易实施并且不需大量增加工程造价 ,是经济且显效的措施