大鼠耳蜗α_(1D)L-型电压门控钙通道组织特异性异构体的剪切方式及其意义

目的　研究α1DL 型电压门控钙通道基因在大鼠耳蜗的剪切 (splicing )方式及其意义。 方法　以显微解剖取材的大鼠耳蜗基底膜为起始材料 ,利用外显子特异性 (exon specific)引物的RT PCR扩增和序列测定确定耳蜗表达的α1DL 型电压门控钙通道的剪切方式。结果　耳蜗表达的α1D钙通道cDNA剪切部位发生在功能域Ⅰ、Ⅱ之间的细胞内连接区和羧基末端。结论　大鼠耳蜗存在α1D钙通道组织特异性的剪切异构体 ,选择性剪切可能是内耳基因表达调控的重要机制     

试谈高速公路工程档案的规范化管理

由于高速公路工程档案的内容和特点不同于一般的文书档案，必须采取针对其特点的措施，实施全过程的管理，才能保证其规范化、标准化。     

A New Proposal of Inverse-Time Characteristics for Overcurrent Relay Based on Microcomputer

The conventional time function of electromechanical relays is hard to coordinate with other relays．In order to promote the application of inverse-time overcurrent relays，a new time function for microprocessor-type relay is proposed．The setting of the trip time for this relay is performed by determining the shortest trip time and the longest trip time，respectively．The results of analysis show that with the new time function，the inverse-time overcurrent relay is easy to coordinate with other relays and has a comparatively shorter trip time，and that the fault happens in the protective zone．     

Performance of Adaptive Web Server with QoS Pre-estimate

The heterogeneity in the communicating terminals needs to be handled through software-supported adaptation. A QoS （quality of service） pre-estimation for user admission control and system load control is proposed. In the QoS pre-estimation, the content quality and wait time characteristics are combined together. The feedback control that acts as an adjuster of the input parameters of the pre-estimate module to improve the estimate accuracy is also described in detail. Test results show that the service quality control mechanism is effective, and the system capacity can be improved.     

Study of the mechanism on the apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase

Objective To investigate the mechanisms of apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase. Methods Human leukemia cell line K562 were exposed to indole-3-acetic acid （IAA） at 20, 40, 60, 80 or 100 mol/L and horseradish peroxidase（HRP） at 1.2 g/mL for varying times. MTT assay was applied to detect the cell proliferation. Flow cytometry was performed to detect the arrest of cell cycle. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） assay was used to measure apoptosis. 2, 7-dichlorofluorescin diacetate （DCFH-DA） uptake was measured to determine free radical by confocal microscope. Content of malondiadehyde （MDA） and activity of superoxide dismutase （SOD） were measured by biochemical methods. Results IAA/HRP initiated growth inhibition of K562 cells in a dose- and time-dependent manner. Flow cytometry revealed that cell cycle arrested at G1/G0 after 24 hours treatment. After 72 hours treatment, apoptotic rate of 100 mol/L IAA group increased to 43.9 %, which was 5 times that of control（P〈0.01）. Content of MDA and activity of SOD increased respectively in treatments compared with control. Meanwhile, IAA/HRP stimulated the formation of free radical, which was increased by IAA concentration-dependently. Conclusion The combination of IAAand HRP can inhibit the growth of Human leukemia cell line K562 in vitro by inducing apoptosis which is associated with the increase of free radical. The combination of IAA and HRP might be a promising chemopreventive and chemotherapeutic agent against human leukemia.     

基于小波变换的船用雷达海杂波小目标检测

强海浪中小目标回波的检测一直是船用雷达目标识别的难点,但现有的统计方法难以准确和全面地描述复杂的海杂波特性.要突破这一局限性,以获得对复杂现象更有效的描述,就迫切要求使用新技术.文中利用小波理论,针对强海浪下点目标和低矮宽大目标二类以往难以检测的目标,通过区分噪声、点目标、低矮宽大目标在小波变换下不同的奇异特征、设计了一套能够识别出点目标和低矮宽大目标的方案.     

非理想电压下有源电力滤波器的电流检测

分析了有源电力滤波器（APF）电流（ip-iq）检测法中影响检测精度的因素，包括低通滤波器和锁相环的频率及相位检测误差．为消除电流检测中非理想电压带来的锁相环检测误差，提出改进的电流（ip-iq）检测法．在该方法中，将APF直流侧电压的调整部分单独考虑，调整量加入到经过低通滤波器滤波并经反变换环节得到的基波电流信息中．这样，未经过反变换的直流侧电压调整值不受锁相环误差的影响．仿真验证了改进方案能有效降低非理想电压对电流检测精度的影响，提高APF性能．     

宽体板拱桥空间受力分析

当板拱桥的宽度与跨度接近，甚至其宽度大于跨度，这类板拱桥称为宽体板拱桥．这类桥梁现有的杆系结构设计理论存在问题．按《公路桥涵设计通用规范（JTGD60-2004）》，以实腹式宽体板拱桥为例，利用MIDAS／Civil建立空间有限元分析模型，进行影响面计算，分析了应力最不利位置以及其值的加载计算，将计算结果与平面杆系有限元简化分析模型的计算结果进行比较．指出二者分析结果的相同的和不同的方面，为该类桥梁的设计、病害分析提供理论基础．     

基于MATLAB及VB的城市交通仿真研究

将MATLAB与VB结合起来,充分发挥各自的技术优势,研发了城市交通仿真系统,包括路口交通仿真、最佳路径仿真等。利用VB建立仿真界面,提供状态参数,调用MATLAB进行数据运算,再把结果送回以供研究。系统投入运行后会很大程度地提高城市交通的运营质量、可视化程度,促进城市的经济发展。     

我国铁路集装箱运输的发展现状探析

为解决当前集装箱在我国铁路运输系统中发展滞后的问题．本文从其基础设施、管理体制、运营组织方面阐述了铁路集装箱运输的发展现状，为我国加强铁路集装箱的管理，进一步改善铁路集装箱运输的运营环境．提供现实依据。最后提出了我国未来发展集装箱运输的几个重点方向。