昆虫激素体外调节家蚕滞育激素受体基因的表达

为了深入研究家蚕滞育的分子机理,从家蚕基因组中扩增了1395 bp和972 bp 2个不同长度的滞育激素受体基因Bmdhr启动子片段,以pGL3.0 Basic为载体,分别构建荧光素酶报告质粒,利用家蚕细胞瞬时表达系统分析其转录活性以及昆虫保幼激素类似物（JHA）、蜕皮激素（20-OH-ecdysone）和滞育激素（DH）对其活性的影响．结果表明：1395 bp的Bmdhr启动子活性显著低于972 bp的Bmdhr启动子,这说明Bmdhr启动子在-941～-1364 nt区间存在负调控元件．JHA浓度为2,4,6μg/mL时,极显著地增强启动子活性;浓度为1μg/mL时活性显著减弱,而为8μg/mL时则活性极显著减弱．20-OH-ecdysone对Bmdhr启动子活性的影响具有剂量效应,低浓度（1,2μg/mL）时显著增强启动子活性;浓度为4μg/mL时,启动子的活性显著减弱;但当浓度继续升高时,启动子活性又极显著增强．DH对Bmdhr启动子活性的影响同样具有剂量效应,其浓度为10～40 nM时,显著增强启动子活性;而当其浓度达到60 nM时,启动子活性变化不显著．     

橡胶粉对混凝土抗冻耐久性的影响

通过室内试验,采用冲击弹性波技术测量混凝土的动弹模,研究了橡胶粉及掺量对混凝土抗冻耐久性的影响.结果表明:与普通混凝土相比,冻融次数较少时,适量掺加橡胶粉,混凝土的质量增加、动弹模降低、相对动弹模增加、抗压强度有所降低,橡胶粉掺量对抗冻性能影响不大;但冻融次数较大时,适量掺加橡胶粉,混凝土的质量损失率减小,动弹模、相对动弹模及抗压强度增加,抗冻性能增强,最佳橡胶粉掺量为15～20 kg/m3.通过扫描电镜试验分析,揭示了掺加橡胶粉提高混凝土抗冻耐久性的微观机理.研究成果可为混凝土抗冻耐久性设计提供理论依据和参考.     

基于氯离子含量与组分分析的海砂特征研究

出于建设品质工程, 规范砂石料市场的需求, 对砂的质量要求逐渐严格。 为有效分辨海砂与河砂, 本文根据规范、 文献与相关试验, 探讨了海砂与河砂在氯离子含量、 化学组成两方面的不同。 通过绘制氯离子含量特征曲线、 进行组分分析试验, 探究了分辨海砂与河砂的方法。 试验表明, 在延长浸泡时间的条件下, 海砂与河砂的氯离子含量存在较大差异。 另一方面, 组分分析的结果表明, 海砂中存在特征性矿物, 是与河砂进行分辨的关键性证据。     

Symmetry based searching process for initial conditions of passive running

Symmetrical passive running of a simple spring model is commonly used to predict the locomotion of real animals and develop the design strategies for legged systems. If the initial conditions for running can be found automatically, we can expand the ranges of the model parameters freely. To achieve this objective, we derive an efficient searching procedure based on analysis of the symmetries. Using this process, the desired initial states of the passive running are obtained. Existence of the results reveals that the leg symmetries can be used to generate the symmetrical running. Moreover, the leg forces are associated with the running pattern, implying that we should choose suitable motion for the given model characteristics.     

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无线传感网是采集实时交通信息的重要工具.因其节点能量有限,必须设计高能效的分簇路由算法以延长网络周期.本文通过建立一种智能交通中无线传感器网络的应用模型,根据其特点和要求分析LEACH协议的优缺点并提出一种能量负载均衡的分簇算法.该算法对LEACH协议当中的簇首机制进行改进,综合考虑候选节点的剩余能量和簇首节点的分布位置;建立簇间多跳路由机制以避免单跳通信的大能量消耗;创造一种簇重构方法,避免过于频繁的簇重构引起的不必要能量消耗.仿真结果表明,能量均衡算法可有效平衡节点能量消耗分布,延长网络生命周期,可很好的应用于基于WSN的ITS当中.     

国内外市域轨道交通车站分布比较研究

大型城市在设置市域轨道交通郊区段与市区段车站时需考虑不同的因素.本文通过分析比较香港、巴黎、东京和上海的典型市域轨道交通线路车站设置案例,得出了如下研究结论:市域线路在市区段若有平行市区轨道交通线路时应采用大站间距设置模式;在郊区段应采用基于客流集散点灵活设站的模式.经过郊区开发密度低的区域时如采用地面或高架的敷设方式有利于越行线的布设,可改进运营组织,提高运营效率.     

A time series analysis of the dynamic competition between major cargo airports

This paper models the dynamic competition between major cargo airports from the time series perspectives of long-term equilibriums and short-run dynamics. We first apply a unit root test to examine the stationarity of cargo throughput data. Airports are then analysed pairwise by region to test their equilibrium relationships through cointegration analysis. Meanwhile, we also utilise the error correction model to investigate the short-run impacts of cargo traffic between the airports. According to our findings, there are four positive long-term equilibriums, one positive short-run dynamic and three negative short-run dynamics in Asia. In addition, the airports in North America are found to have four positive long-term equilibriums, three negative long-run equilibriums, seven positive short-run impacts and three negative short-run impacts. As these dynamic relationships imply competition between cargo airports, our study can provide airport authorities with the reference to develop their long-term strategies and short-run operational plans.     

沥青检测指标统计与试验影响因素分析

通过统计分析大量沥青指标数据,总结了沥青指标中的受条件影响显著的指标,以及试验操作中需要注意的操作事项。     

Practical approach for travel time estimation from point traffic detector data

Accurate estimation of travel time is critical to the success of advanced traffic management systems and advanced traveler information systems. Travel time estimation also provides basic data support for travel time reliability research, which is being recognized as an important performance measure of the transportation system. This paper investigates a number of methods to address the three major issues associated with travel time estimation from point traffic detector data: data filling for missing or error data, speed transformation from time‐mean speed to space‐mean speed, and travel time estimation that converts the speeds recorded at detector locations to travel time along the highway segment. The case study results show that the spatial and temporal interpolation of missing data and the transformation to space‐mean speed improve the accuracy of the estimates of travel time. The results also indicate that the piecewise constant‐acceleration‐based method developed in this study and the average speed method produce better results than the other three methods proposed in previous studies. Copyright © 2011 John Wiley & Sons, Ltd.     

高水压盾构隧道管片接缝防水可靠性试验研究

盾构隧道管片衬砌防水至关重要,而在东亚地区大部分盾构隧道都采用单层衬砌,由于没有内衬,则弹性密封垫的可靠性变得更为关键.作者以南京市纬三路过江隧道工程为依托,研制了全新的高水压、全自动三向加载防水性能试验系统,并运用工程实际1∶1的弹性密封垫和混凝土试件,进行了多组密封垫装配力及一字缝、T字缝防水性能试验,从而研究优化出了性能、结构优异的三元乙丙橡胶弹性密封垫断面形式.试验结果表明,密封垫装配力与其防水性能存在很大程度上的相关性;密封垫的硬度、沟槽填充率在不同程度上对密封垫防水性能有直接影响.