应用流式细胞术检测17β-雌二醇对H2O2诱导的视网膜神经细胞凋亡的保护作用

目的用流式细胞技术(FACS)检测H2O2诱导的视网膜神经细胞凋亡以及17β-雌二醇(βE2)对细胞的保护作用。方法取新生24 h内的SD大鼠进行视网膜神经细胞的原代培养,待细胞培养4-5 d,经不同因素处理后,用MTT法检测细胞存活率,用FACS法检测细胞凋亡率。结果200μmol/L的H2O2能有效诱导视网膜神经细胞凋亡;低浓度的H2O2不能诱导细胞凋亡,但高浓度的H2O2诱导细胞凋亡的同时也导致了正常细胞比率的锐减;10μmol/L的βE2能有效对抗H2O2诱导的视网膜神经细胞凋亡;低浓度的βE2对细胞未表现出保护作用,高浓度的βE2对细胞反而表现出毒性作用。结论一定浓度的βE2在一定范围内能对抗H2O2诱导的视网膜神经细胞凋亡,表现对神经细胞保护的作用。     

Singular perturbation composite control of a free-floating flexible dual-arm space robot

The Free-floating Flexible Dual-arm Space Robot is a highly nonlinear and coupled dynamics system. In this paper, the dynamic model is derived of a Free-floating Flexible Dual-arm Space Robot holding a rigid payload. Furthermore, according to the singular perturbation method, the system is separated into a slow subsystem representing rigid body motion of the robot and a fast subsystem representing the flexible link dynamics. For the slow subsystem, based on the second method of Lyapunov, using simple quantitative bounds on the model uncertainties, a robust tracking controller design is used during the trajectory tracking phase. The optimal control method is designed in the fast subsystem to guarantee the exponential stability. With the combination of the two above, the system can track the expected trajectory accurately, even though with uncertainty in model parameters, and its flexible vibration gets suppressed, too. Finally, some simulation tests have been conducted to verify the effectiveness of the proposed methods.     

内河航道横流对船舶航行的影响

为了使船舶能安全通过航道内的横流区域, 利用水槽进行遥控自航船模试验, 提出了内河航道横流对船舶航行横漂速度、漂角、航迹带宽度和漂距影响的经验公式, 分析了Ⅳ与Ⅴ级航道横向流速的限值范围。分析结果表明: 横流对船舶航行的影响程度主要与对岸航速成反比, 与横流的大小及区域长度成正比, 与船型大小(航道等级)成反比, 同时与驾驶员的航行经验和初始船位有关; 在限制航路航行方式过程中, Ⅳ与Ⅴ级航道对岸航速为2、3、4m·s^-1时, 可克服的一个船长内横流限值为0.48、0.58、0.70m·s^-1。     

A data model applied to the knowledge-based ICAS

Image-guided computer aided surgery system (ICAS) contributes to safeness and success of surgery operations by means of displaying anatomical structures and showing correlative information to surgeons in the process of operation. Based on analysis of requirements for ICAS, a new concept of clinical knowledge-based ICAS was proposed. Designing a reasonable data structure model is essential for realizing this new concept. The traditional data structure is limited in expressing and reusing the clinical knowledge such as locating an anatomical object, topological relations of anatomical objects and correlative clinical attributes. A data structure model called mixed adjacency lists by octree-path-chain (MALOC) was outlined, which can combine patient's images with clinical knowledge, as well as efficiently locate the instrument and search the objects' information. The efficiency of data structures was analyzed and experimental results were given in comparison to other traditional data structures. The result of the nasal surgery experiment proves that MALOC is a proper model for clinical knowledge-based ICAS that has advantages in not only locating the operative instrument precisely but also proving surgeons with real-time operation-correlative information. It is shown that the clinical knowledge-based ICAS with MALOC model has advantages in terms of safety and success of surgical operations, and help in accurately locating the operative instrument and providing operation-correlative knowledge and information to surgeons in the process of operations.     

后张预应力混凝土梁管道摩阻测试研究

介绍了预应力管道摩阻损失的测试原理与测试方法,结合后张预应力混凝土简支梁和连续梁的管道摩阻实测结果,用最小二乘原理识别管道摩阻系数和偏差系数,分析了测试方法的合理性和试验结果的可靠性。探讨目前长束单端张拉中,在千斤顶行程不足的情况下,准确获得预应力钢束中预张力的方法,并将实测结果与设计和施工规范要求进行了对比分析,得出了一些有价值的结论。     

基于遗传算法的混凝土热学参数反分析与反馈研究

混凝土热学参数主要是通过室内试验得到的,不能真实地反应施工现场的混凝土热学性能.针对这一问题,结合工程现场实测的混凝土温度,利用遗传算法对混凝土温度场进行反演计算,并将计算值和实测值进行对比,分析计算结果的合理性,得到反映混凝土真实热学性能的参数.结合混凝土温度场应力场的基本原理和水管冷却的精确算法,利用这些参数,通过三维有限元仿真计算程序对施工现场混凝土温度场进行反馈计算.确定温控防裂措施,指导后续施工.结果表明t该方法可成为替代用室内试验和经验公式选取热学参数的有效途径.     

时空信息多模式融合模型应用研究

给出了时空多模式融合模型(MM模型)的实用建模方法.利用华北地震和北京SARS 2个时空数据集对MM模型进行了建模应用和性能评价,与单纯神经网络模型相比,MM模型的预测准确性与预测趋势变化的能力均有所提高.分析表明,利用MM模型对不同类型时空过程建模时,不同分量信息所起到的作用并不相同.     

温度对混凝土箱梁应力和变形影响的测试与分析

为了研究混凝土箱粱在施工期问的温度场和温度随时问的变化规律.对内蒙古磴口黄河大桥进行了现场测试.分析了混凝土箱梁桥结构的温度效应及温度场对其挠度和应力的影响规律.结果表明:在结构荷载不变的情况下.箱梁的温度应力较易测得;温度对箱梁的应力和挠度均有较大影响,在设计中应予以重视.对如何在混凝土箱梁的施工和设计中考虑温度影响提出了建议.     

NT4-Ant-Shepherdin[79-87]融合基因重组载体的构建

目的设计构建携带NT4-Ant-Shepherdin[79-87]的cDNA融合基因的重组载体,为针对Survivin的靶向治疗奠定基础。方法应用非对称引物/模板法、PCR技术制备NT4-Ant-Shepherdin[79-87]cDNA片断,连接于pGEM-T-Easy载体,经克隆测序、酶切后与PBV220/NT4质粒连接;转化感受态细胞E.coliDH5α,亚克隆获得NT4-Ant-Shepherdin[79-87]融合基因。结果克隆出Ant-Shepherdin[79-87]基因,经酶切及测序证实结果正确;连接PBV220/NT4,经克隆、酶切,琼脂糖凝胶电泳证实获得321bp的NT4-Ant-Shepherdin[79-87]目的基因片断。结论通过分子生物学技术成功构建了携带NT4-Ant-Shepherdin[79-87]融合基因的重组载体,为进一步研究针对Survivin的靶向抗肿瘤作用奠定了基础。