β淀粉样肽1～42单克隆抗体的制备

目的　制备稳定分泌β -淀粉样肽 1～ 4 2 (Aβ1-42 )单克隆抗体的杂交瘤细胞 ,产生高效价抗体。方法　通过基因工程技术 ,将Aβ1-42 基因融合于乙肝核心抗原 (HBcAg)的主要免疫优势区 (MIR) ,制备Aβ和HBcAg的融合蛋白 ,免疫BALB/c小鼠 ,取脾细胞与SP2 / 0细胞融合 ,筛选能稳定分泌Aβ1-42 单克隆抗体的杂交瘤细胞。结果　得到两株能稳定分泌Aβ1-42 单克隆抗体的杂交瘤细胞 ,Aβ1-42 单克隆抗体的Ig亚类为IgG3 。结论　制备得到的Aβ1-42 单克隆抗体特异性强 ,效价高     

银杏叶提取物防治肝纤维化的实验研究

目的　探讨银杏叶提取物 (EGb)对慢性肝炎的防治作用及其机制。方法　SD大鼠随机分为正常组、模型组、EGb防治组和秋水仙碱组。用四氯化碳皮下注射法建立慢性肝损伤模型 ,分别用生理盐水或药物灌胃治疗。对比观察EGb对慢性肝损伤大鼠肝纤维化和脂质过氧化指标的影响 ,结合肝病理组织学及肝组织内Ⅰ、Ⅲ型胶原表达的变化加以探讨。结果　EGb防治组大鼠肝组织中PCⅢ、透明质酸、层黏连蛋白和CⅣ型胶原含量明显降低 ,血中过氧化物歧化酶、谷胱甘肽过氧化物酶活性明显升高 ,丙二醛含量明显降低 ,与模型组比较差异均有显著性意义 (P <0 .0 5或P <0 .0 1) ;形态学观察 ,EGb防治组大鼠肝组织损伤明显轻于模型对照组和秋水仙碱对照组 :HE染色显示肝组织炎症损伤减轻 ,VG染色显示肝组织纤维化程度明显减低 ,免疫组化染色显示肝组织中Ⅰ、Ⅲ型胶原表达明显减少。结论　EGb具有抑制脂质过氧化损伤 ,防治大鼠实验性肝纤维化的作用。     

糖尿病与高血压病患者血管内皮功能的变化特点

目的　利用多普勒超声检测糖尿病与高血压病的血管内皮功能 ,评价其变化特点。方法　运用多普勒超声对2 5例糖尿病 (DM )及 2 5例高血压病 (EH)患者和 2 0例正常人肱动脉充气加压及舌下含服硝酸甘油前后血管直径的变化进行观察。结果　DM和EH组充血状态下内径扩张程度较正常对照组明显减低。含服硝酸甘油后DM组内径扩张程度较正常对照组明显减低 ,EH组无显著性改变。结论　DM患者存在内皮依赖性舒张功能及非依赖性舒张功能障碍 ,EH患者内皮依赖性舒张功能障碍 ,非依赖性舒张功能正常     

TSH RECEPTOR GENETIC ALTERATIONS IN THE AUTONOMOUSLYFUNCTIONING THYROID ADENOMAS

Objective To determine the relationship between TSH receptor gene mutations and autonomously functioning thyroid adenomas (AY‘]rAs). Methods The thyroid samples from 14 cases of diagnosed AFTAs were analyzed, with normal thyroid specimens adjacent to the tumors as controls. The 155 base pairs DNA fragments which encompassed the third cytoplasmic loop and the sixth transmembrane segments in the TSH receptor gene exon 10 were amplified by Polymerase chain reaction (PCR) and analyzed by the single-strand conformation polymorphism (SSCP). Direct sequencing of the PCR products was performed with Prism Dye Terminator Cycle Sequencing Core Kit.Results 6 of 14 AFTA specimens displayed abnormal migration in SSCP analysis. In sequence analysis of 3 abnormally migrated samples, one base substitution at nucleotide 1957 (A to C) and two same insertion mutations of one adenosine nucleotide between nucleotide 1972 and 1973 were identified. No mutations were found in controls. Conclusion This study confirmed the presence of TSH receptor gene mutations in AFTAs; both one-point substitution mutation and onebase insertion mutation were found to be responsible for the pathogenesis of AFTAs.     

DESIGN OF A DIGITAL SIGNATURE SCHEME BASED ON FACTORING AND DISCRETE LOGARITHMS

Objective Focusing on the security problem of authentication and confidentiality in the context of computer networks, a digital signature scheme was proposed based on the public key cryptosystem. Methods Firstly,the course of digital signature based on the public key cryptosystem was given. Then, RSA and ELGamal schemes were described respectively. They were the basis of the proposed scheme. Generalized ELGamal type signature schemes were listed. After comparing with each other, one scheme, whose Signature equation was (m r)x=j s modФ(p) , was adopted in the designing. Results Based on two well-known cryptographic assumptions, the factorization and the discrete logarithms, a digital signature scheme was presented. It must be required that s“ was not equal to p‘q“ in the signing procedure, because attackers could forge the signatures with high probabilities if the discrete logarithms modulo a large prime were solvable. The variable public key “e“ is used instead of the invariable parameter “3“ in Ham‘s signature scheme to enhance the security. One generalized ELGamal type scheme made the proposed scheme escape one multiplicative inverse operation in the signing procedure and one modular exponentiation in the verification procedure.Conclusion The presented scheme obtains the security that Harn‘s scheme was originally claimed. It is secure if the factorization and the discrete logarithms are simultaneously unsolvable.     

EXPRESSION OF NESTIN AND GLIAL FIBRILLARY ACIDIC PROTEIN IN DIFFERENT PERIOD AFTER SPINAL INJURY IN ADULT RATS

Objective To study the expression of Nestin and glial fibrillary acidic protein (GFAP) in different period after spinal injury in adult rats. Methods Animal moels were created by artery clamp. Expression of Nestin and GFAP in different period ( 1,3,5days; 1-8 weeks) and different area( injury locus and its surrounding tissue ) after spinal injury were observed pathologicaly using immunofluorescence and LeicaQ5001W imaging processing system.Results There was expression of Nestin and GFAP in injured area 1 day after injury. The expression increased not only in in injured area but its sourrounding 3--7 days later and gradually got to peak value. As the time went on, expression of Nestin and GFAP dereased. Conclusion Spinal injury can induce the expression of Nestin. Nerve stem cell has response to spinal injury. There is positive correlation between expression of Nestin and hyperplasia of reactivity astrocyte. Nerve stem cell maybe invovled in the repair of central nervous system (CNS).     

THE GENE EXPRESSION OF BDNF IN NORMAL RABBIT RETINA

Objective To investigate the distribution of brain-derived neurotrophic factor(BDNF) protein in the rabbit retina. Methods Immune response material in the retina was observed using BDNF antibody by the method of immunohistochemistry. Results BDNF gene expression was mainly found in the RGCs, also in innernuclei cells and outernuclei cells in rabbit retina. Conclusion RGC is not only the target cell of BDNF, but also express the BDNF protein. BDNF from multi-sources participates in the regulation of RGCs.     

城市交通与信息化

信息技术的不断发展为城市交通运行改善带来新的契机,城市交通与信息化是一个对中国未来城镇化模式影响非常深远的命题。首先从道路交通管理、公共交通服务、交通综合信息平台等六个方面阐述中国交通信息化的发展状况,总结交通信息化在信息采集、数据分析、决策支持、信息发布以及社会关注五个方面的发展趋势。指出交通信息化应更关注城市交通、都市圈交通的特点,在认识城市交通本质和服务对象的基础上进行总体设计。最后,强调智能交通信息化发展目标应与城市整体发展目标相一致,应考虑公众、企业、政府层面的不同诉求进行交通信息化的顶层设计。     

基于恒载零弯矩的大跨曲线连续刚构桥线形分析

针对大跨曲线连续刚构桥弯扭耦合导致结构线形施工控制异常复杂的问题,在总结曲线刚构桥线形影响因素的基础上,提出了施工恒载零弯矩的大跨曲线连续刚构桥线形控制系统和具体实现措施。为验证所提措施的有效性,以曲率半径1 200 m、主跨80 m的江西会昌九岭高架桥为例,采用有限元法对不同曲率半径下增加预应力束或临时斜拉塔辅助合龙实现恒载零弯矩的措施与传统设计进行了分析对比。结果表明:增加预应力束,或者临时斜拉塔辅助合龙可大大减少结构的竖向位移、横向位移和扭转位移,可以简化大跨曲线连续刚构桥施工线形控制的难度。     

矩阵方程AX=B的自反解与反自反解及最佳逼近

给定两个广义反射矩阵P,Q,通常对于矩阵方程AX=B关于P,Q的自反解和反自反解的研究大多是通过矩阵分解或广义奇异值分解来进行的。采用广义逆,建立该方程存在自反解和反自反解的充要条件以及解的一般表达式,并研究与之相关的矩阵最佳逼近问题。