大鼠耳蜗α_(1D)L-型电压门控钙通道组织特异性异构体的剪切方式及其意义

目的　研究α1DL 型电压门控钙通道基因在大鼠耳蜗的剪切 (splicing )方式及其意义。 方法　以显微解剖取材的大鼠耳蜗基底膜为起始材料 ,利用外显子特异性 (exon specific)引物的RT PCR扩增和序列测定确定耳蜗表达的α1DL 型电压门控钙通道的剪切方式。结果　耳蜗表达的α1D钙通道cDNA剪切部位发生在功能域Ⅰ、Ⅱ之间的细胞内连接区和羧基末端。结论　大鼠耳蜗存在α1D钙通道组织特异性的剪切异构体 ,选择性剪切可能是内耳基因表达调控的重要机制     

OCCURRENCE CHARACTERISTIC OF KASHIN-BECK DISEASE BASED ON NUCLEAR FAMILY PEDIGREES

Kashin Beckdisease (KBD )isanendemicos teochondropathyresultingfromimpairedendochon drialdevelopmentinchildren .Itmanifestswithsecondarychronicdeformitiesandosteoarthrosisinadults.Geographically ,thediseaseoccursmainlyinadiagonalbeltrangingfromthenortheasttothesouthwestofChina (KBDarea) .Thisareaischar acterizedbyalowfoodseleniumcontent .Thereare2 .5millionpatientswithKBDand 30millionpeo pleareatrisk[1] .TheclinicalmanifestationsofKBDareflexionoftheterminalpartofthefingersorcrookedfinger…     

长三角城际客运专线运输组织方案及通过能力计算

本文分析提出了“站站停“普客和“大站停”快客两种列车相结合的运营模式，并在此基础上得出了计算大站停特快列车扣除系数和相应区间通过能力的方法。     

BRT速度影响因素分析及提高措施

本文分析了影响BRT速度的各个因素和计算方法,并提出提高BRT速度的改进措施,使在我国BRT规划实施前,全面掌握和了解影响速度的各个环节,以期能够在实际中顺利地达到快速的目的.     

Study on lift-up speed of aerodynamic compliant foil thrust bearings

Objective The experimental study on the lift-up speed of a new kind of compliant aerodynamic foil thrust hearings was performed on the multifunetional test rig established for testing the performances of foil gas bearings. Methods The lift-up speed of foil gas thrust hearing under given axial load was analyzed through the spectrum of axial displacement response in frequency domain. Results The test results indicated that the difference in the spectrum of axial displacement responses before and after lifting up of the rotor was obvious. After lifting up of the rotor, there were only larger components of rotation frequency and lower harmanie frequencies. If the rotor wasn＇t lift-up, there were also larger components of other frequencies in the spectrum. Conclusion So by analyzing the spectrum of axial displacement response, the results showed that the lift-up speed was about 1 860 rpm when the axial load was 31N.     

Study of the mechanism on the apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase

Objective To investigate the mechanisms of apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase. Methods Human leukemia cell line K562 were exposed to indole-3-acetic acid （IAA） at 20, 40, 60, 80 or 100 mol/L and horseradish peroxidase（HRP） at 1.2 g/mL for varying times. MTT assay was applied to detect the cell proliferation. Flow cytometry was performed to detect the arrest of cell cycle. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） assay was used to measure apoptosis. 2, 7-dichlorofluorescin diacetate （DCFH-DA） uptake was measured to determine free radical by confocal microscope. Content of malondiadehyde （MDA） and activity of superoxide dismutase （SOD） were measured by biochemical methods. Results IAA/HRP initiated growth inhibition of K562 cells in a dose- and time-dependent manner. Flow cytometry revealed that cell cycle arrested at G1/G0 after 24 hours treatment. After 72 hours treatment, apoptotic rate of 100 mol/L IAA group increased to 43.9 %, which was 5 times that of control（P〈0.01）. Content of MDA and activity of SOD increased respectively in treatments compared with control. Meanwhile, IAA/HRP stimulated the formation of free radical, which was increased by IAA concentration-dependently. Conclusion The combination of IAAand HRP can inhibit the growth of Human leukemia cell line K562 in vitro by inducing apoptosis which is associated with the increase of free radical. The combination of IAA and HRP might be a promising chemopreventive and chemotherapeutic agent against human leukemia.     

AN OPTIMAL SELF-SCALING STRATEGY TO THE MODIFIED SYMMETRIC RANK ONE UPDATING

1 Introduction Inthispaper,weconsidertheunconstrained optimizationproblem minf(x)(1.1) wheref(x):Rn→Ristwicecontinuouslydifferen tiable,andasolutionx totheproblemexists.Qua si Newtonmethodsforsolvingproblem(1.1)are widelyapplied.Startingfromaninitialpo…     

改良的地高辛负荷量疗法

本文针对目前临床上广泛使用的地高辛维持量疗法存在的问题,运用药物动力学的有关理论,提出了改良的负荷量疗法。文章通过血浆地高辛浓度和临床观察,比较两种疗法的临床疗效和中毒发生率。结果表明改良的地高辛负荷量疗法能提高疗效,具有减少洋地黄中毒的趋势。本文推荐的地高辛负荷量疗法为:第1天给予负荷量0.375mg～0.75mg,分2～3次服用;第2天后按Cr调整维持量。     

新型玻璃幕墙式登机桥结构有限元分析

以ANSYS有限元分析软件为工具，对机场新型玻璃幕墙式旅客登机桥的结构进行有限元分析，得到结构在3种工况下的应力和变形，并对结构的强度和刚度进行了校核，在此基础上对结构作了相应的改进，使其满足设计要求．另外，还对有限元模型建立和载荷处理中的耦合及载荷的近似问题进行了探讨．     

基于WEP协议的无线局域网安全性分析与测试

随着WLAN技术的发展成熟，其安全性正日益受到人们的重视，由于WLAN使用的无线媒体具有开放信道的特点，因此其对安全性的要求比在有线环境下更高也更严格，作为目前使用最广泛的无线局域网标准，IEEE802．11b使用WEP协议来保证无线网络环境下的数据安全，文中简要介绍WEP协议的实现原理，着重分析了WEP存在的安全隐患，并利用Airsnort软件对其进行了安全性测试．