肿瘤浸润淋巴细胞和重组人白细胞介素-2对进展期肿瘤患者细胞免疫功能的影响

对20例经手术切除的进展期肿瘤患者用活化的肿瘤浸润淋巴细胞（TIL）和重组人白细胞介素-2（rhIL－2）治疗，检测治疗前后细胞免疫功能变化。其中8例治疗后PBL对同种异体相同组织学类型靶细胞的细胞毒性从43．2升高到1249．0溶解单位（P＜0．05），而NK活性无明显变化；T4、T8淋巴细胞亚群荧光强度明显升高，从1．9到3．9（P＜0．05）及4．4到7．2（P＜0．05）。所有患者PPD及PHA反应性明显增强，从10．9mm到13．6mm（P＜0．01）及7．9mm到12．7mm（P＜0．01），15例有一过性寒战、发热，其中10例伴轻度恶心、呕吐。结果提示：TIL和rhIL－2治疗后可明显提高进展期肿瘤患者的细胞免疫功能，而无明显毒副作用，可能成为进展期肿瘤术后重要的辅助治疗方法。     

EFFECTS OF NITRIC OXIDE SYNTHESIS INHIBITION WITH OR WITHOUT NITRIC OXIDE IN

ＦＦＥＣＴＳＯＦＮＩＴＲＩＣＯＸＩＤＥＳＹＮＴＨＥＳＩＳＩＮＨＩＢＩＴＩＯＮＷＩＴＨＯＲＷＩＴＨＯＵＴＮＩＴＲＩＣＯＸＩＤＥＩＮＨＡＬＡＴＩＯＮＯＮＲＥＳＰＯＮＳＥＳＴＯＳＹＳＴＥＭＩＣＣＯＣＡＩＮＥＡＤＭＩＮＩＳＴＲＡＴＩＯＮＩＮＲＡＴＳ．Ｅ．Ｈｅ...     

连续梁桥收缩徐变试验研究

在试验室内及工地环境中对某连续梁桥进行收缩徐变试验,得到了该桥的实测收缩徐变数据,拟合出其收缩徐变规律,将其与几种现行规范相比较,分析其吻合程度,验证规范的适用性。     

轮胎对汽车油耗的影响

轮胎是汽车的重要组成部件之一。正确的使用轮胎不但能延长其使用里程而且还能减少汽车油耗。为此，人们在轮胎对汽车油耗的影响方面，做了大量的试验研究，并已取得了相当多的理论知识和丰富实践经验。     

葡萄糖、胰岛素和尿酸对猪近端肾小管上皮细胞株血红素氧合酶-1的影响

目的观察不同浓度葡萄糖、胰岛素和尿酸的培养条件下猪近端肾小管上皮细胞株(LLC-PK1)血红素氧合酶(HO-1)活性的改变,以探讨代谢相关因素在细胞水平对肾小管上皮细胞的影响。方法以LLC-PK1为研究对象,观察葡萄糖浓度为5、10、22、33 mmol/L,胰岛素浓度为0、10-9、10-8、10-7mol/L,尿酸浓度为0、0.1、0.2、0.4 mmol/L培养48 h条件下,LLC-PK1细胞HO-1活性的改变。结果0.1 mmol/L0、.2 mmol/L和0.4 mmol/L尿酸刺激48 h后,LLC-PK1的HO-1活性增加,并呈剂量依赖关系,而上述浓度的葡萄糖和胰岛素刺激48 h对LLC-PK1的HO-1活性无影响。结论一定浓度的尿酸可使LLC-PK1的HO-1活性升高,提示尿酸在细胞水平对肾小管上皮细胞的氧化应激过程产生影响。     

复发转移性乳腺癌mdr-1基因产物P-糖蛋白表达的临床意义

目的　探讨复发转移性乳腺癌mdr 1基因表达产物P 糖蛋白 (P glycoprotein ,P gp)表达及其与含吡喃阿霉素联合方案化疗疗效的关系。方法　应用SABC法检测 4 6例转移性乳腺癌术后组织中P gp表达 ,使用环磷酰胺、吡喃阿霉素和 5 氟尿嘧啶联合方案化疗 ,对比分析P gp表达与疗效的关系。 结果　① 4 6例患者mdr 1基因表达产物P gp阳性表达率 5 6 .5 % ,肝或肺转移者阳性表达明显高于皮肤或淋巴结转移者 (P =0 .0 4 9)。②可评价疗效的 4 3例患者化疗有效率 5 8.1% ;P gp阴性组疗效 (89.5 % )明显优于P gp阳性组 (30 .0 % ) (P <0 .0 1)。③有肝或肺转移者有效率(4 0 .7% )明显低于皮肤或浅表淋巴结转移者 (87.5 % ) (P <0 .0 5 )。④术后曾接受辅助性CAF或CMF方案化疗者复发转移后化疗疗效 (71.4 %和 37.5 % )无显著性差异 (P =0 .0 5 2 )。结论　mdr 1基因表达产物P gp表达阳性者应选择非MDR型药物的联合方案化疗     

增龄相关听力丧失小鼠耳蜗毛细胞表型与基因突变的关系

目的　观察增龄相关听力丧失 (AHL)小鼠耳蜗毛细胞病理改变的超微结构 ,为老年性聋病理生理及临床表现+基因表型提供形态学改变依据。方法　应用扫描电镜 (SEM)观察BALB/c小鼠耳蜗Corti器毛细胞的超微结构改变 ,测定动物ABR反应阈值。DNA提取PCR扩增分析基因型。结果　 6月龄BALB/c小鼠耳蜗底回内外毛细胞有连续的缺失 ,静纤毛束有融合、散乱、变短和失去劲度。ABR阈值呈中到重度听力损失。证明种系特异的Ahl基因位点和基因功能。结论　耳蜗毛细胞静纤毛束融合、散乱、变短是老年性聋早期的主要病理改变 ,mdfw与Ah1的表现形式是同一基因位点 ,可能与myosin基因突变有关。     

铁路单线特长隧道快速施工组织探究

蒙华铁路连云山隧道为重载铁路单洞单线隧道,独头掘进距离长、断面小,洞内施工交通干扰大,隧道快速施工组织难度高。为有效解决单线小断面隧道快速施工组织问题,基于隧道开挖、仰拱及二次衬砌各施工工序组织,对配套施工机械设备、工艺工法选择和施工交通组织等进行研究。通过优化和改进,较大程度上解决了单线特长隧道的快速施工难题,有效提高了单线隧道的施工效率。     

Switch Panel wear loading – a parametric study regarding governing train operational factors

The acting forces and resulting material degradation at the running surfaces of wheels and rail are determined by vehicle, track, interface and operational characteristics. To effectively manage the experienced wear, plastic deformation and crack development at wheels and rail, the interaction between vehicle and track demands a system approach both in maintenance and in design. This requires insight into the impact of train operational parameters on rail- and wheel degradation, in particular at switches and crossings due to the complex dynamic behaviour of a railway vehicle at a turnout. A parametric study was carried out by means of vehicle-track simulations within the VAMPIRE® multibody simulation software, performing a sensitivity analysis regarding operational factors and their impact on expected switch panel wear loading. Additionally, theoretical concepts were cross-checked with operational practices by means of a case study in response to a dramatic change in lateral rail wear development at specific switches in Dutch track. Data from train operation, track maintenance and track inspection were analysed, providing further insight into the operational dependencies. From the simulations performed in this study, it was found that switch rail lateral wear loading at the diverging route of a 1:9 type turnout is significantly influenced by the level of wheel–rail friction and to a lesser extent by the direction of travel (facing or trailing). The influence of other investigated parameters, being vehicle speed, traction, gauge widening and track layout is found to be small. Findings from the case study further confirm the simulation outcome. This research clearly demonstrates the contribution flange lubrication can have in preventing abnormal lateral wear at locations where the wheel–rail interface is heavily loaded.     

WLTC和CLTC-P循环工况及其排放污染物和油耗分析

当前中国轻型车排放和油耗认证循环工况正处于NEDC(New European Driving Cycle,新欧洲驾驶循环)和WLTC(Worldwide Light-duty Test Cycle,全球统一轻型车辆测试循环)并行、CLTC(China Light-duty Vehicle Test Cycle,中国轻型汽车行驶工况)逐步导入的特殊时期。对WLTC和CLTC-P(China Light-duty Vehicle Test Cycle-Passenger,中国乘用车行驶工况)进行分析,对比两种不同驾驶工况的特点,并选取同一辆车进行WLTC和CLTC-P排放污染物和油耗测试,对两种循环下排放和油耗测试结果进行比较,分析不同工况下整车排放和油耗特性,为后续整车开发和标定提供工程参考。