Effects of Panax notoginseng saponins on hydrogen peroxide-induced apoptosis in cultured rabbit bone marrow stromal cells

Objective To investigate the effects of Panax notoginseng saponins (PNS) on hydrogen peroxide (H2O2)-induced apoptosis in cultured rabbit bone marrow stromal cells (BMSCs). Methods BMSCs from 3-month-old New Zealand rabbits were isolated and cultured by the density gradient centrifugation combined with adherent method. The cultured BMSCs were divided into three groups: normal control, H2O2 treatment (100μmol/L), and PNS pretreatment (0.1g/L). Intracellular reactive oxygen species (ROS) levels as the index of oxidative stress were measured by using 2'7'-dichlorodihydrofluorescein diacetate. Flow cytometry was used to observe the apoptosis of BMSCs by staining with annexinV-FITC/PI. The protein expression of Bax in BMSCs was analyzed by Western blotting. Activity of caspase-3 enzyme was measured by spectrofluorometry. Results Pretreatment with PNS significantly decreased intracellular ROS level induced by H2O2 (P<0.01). PNS markedly attenuated H2O2-induced apoptosis rate from 38.68% to 19.24%(P<0.01). PNS reversed H2O2-induced augmentation of Bax expression. Furthermore, PNS markedly reduced the altered in activity of caspase-3 enzyme induced by H2O2(P<0.01). Conclusion PNS has a protective effect on hydrogen peroxide-induced apoptosis in cultured rabbit BMSCs by scavenging ROS and decreasing Bax expression and caspase-3 activity.     

浮动式码头防冲设备在军舰防冲保障中的应用研究

阐述了浮动式码头防冲设备的概念和应用现状,介绍了浮动式码头防冲设备的利于军舰维修保养、隔离防护、减缓摩擦等主要作用,分析了浮动式码头防冲设备主要类型及其具有的随水位升降、构成材料种类多、组合性能好等特点,指出了影响浮动式码头防冲设备设计和使用的主要因素,如载荷、外形、面积、稳定性等,为浮动式码头防冲设备在军舰防冲保障中的应用研究提供了详实资料。     

基于GPS/GIS/GPRS的长途客车主动监控管理软件

针对目前长途客车主动监控管理软件需要人工抽检,自动化程度低和管理落后的现状,提出基于GPS/GIS/GPRS的长途客车主动监控管理系统软件的设计方法。在分析监管系统原理和组成的基础上,采用delphi＋MapX进行软件开发,实现地图操作、异常车辆显示、车辆通讯等功能,并建立系统数据库,完成对车辆异常情况的检索统计及分析。实车试验表明：该文提出的设计方法能提高对长途客车的监控效率和管理水平。     

Probabilistic model-based silhouette refinement for gait recognition

An algorithm to refine and clean gait silhouette noises generated by imperfect motion detection techniques is developed,and a relatively complete and high quality silhouette is obtained.The silhouettes are sequentially refined in two levels according to two different probabilistic models.The first level is within-sequence refinement.Each silhouette in a particular sequence is refined by an individual model trained by the gait images from current sequence.The second level is between-sequence refinement.All t...     

Mesh motion approach based on spring analogy method for unstructured meshes

Mesh motion strategy is one of the key points in many fluid-structure interaction problems. One popular technique used to solve this problem is known as the spring analogy method. In this paper a new mesh update approach based on the spring analogy method is presented for the effective treatment of mesh moving boundary problems. The proposed mesh update technique is developed to avoid the generation of squashed invalid elements and maintain mesh quality by considering each element shape and grid scale to th...     

某低重心独塔斜拉桥抗震性能研究

某黄河独塔斜拉桥初步设计方案有半漂浮结构体系和固定铰支承结构体系。对其两种不同结构体系进行了抗震性能分析,通过对两种不同结构体系地震荷载作用下主塔控制截面内力响应对比可知,该桥为典型低重心独塔斜拉桥,该桥固定铰支承结构体系主塔控制截面的弯矩响应明显低于半漂浮结构体系主塔相应截面的弯矩响应。为进一步提高该桥的抗震性能,分别对不同塔梁之间纵向特殊连接装置对抗震性能的影响进行了优化对比分析,得到了一些有意义的结论。     

高墩部分斜拉桥非线性稳定分析

论述了高墩结构的两类稳定问题,在考虑了几何非线性和材料非线性的基础上,结合仙神河斜拉桥的工程实例,利用大型通用有限元程序ANSYS,选取各种单元建立有限元模型,对比分析了该部分斜拉桥在最高裸墩状态和最大悬臂状态下的稳定性。通过计算分析可知,高墩结构的非线性影响很大,考虑非线性影响的稳定分析对于指导工程实践具有更好的实际意义。     

新型飞鸟式系杆拱桥的方案设计研究与分析

飞鸟式系杆拱桥是一种造型独特的桥型.结合宁波市奉化江大桥的方案设计情况,介绍了该桥的结构设计及施工方法,进行了静力计算分析和结构优化并给出了稳定性分析和动力特性分析结果.计算结果表明该桥受力安全可靠,具有良好的稳定性和动力特性.     

基于新型冠状病毒传播机理的交通出行易感度研究

为科学判断各交通方式出行过程中人群感染病毒的风险概率进而有效控制病毒传播,借助空气传染病模型,根据已发布的新型冠状病毒传播机理,构建了交通出行的病毒易感度评估模型,模型包含对病毒传播有重要影响的通风、载客密度、暴露时间等主要参数。选用各种运输方式中有代表性的运载工具进行不同假设情景下的易感度评估,结果表明,在各种有效防控措施均得以采用的情景下,乘坐交通工具的易感度相比高危情景降低97%以上,相比基准情景降低93%以上。研究可为交通领域的科学防疫、精准施策提供依据。