氧化苦参碱对慢性肾脏病患者血清TGF-β1和Col Ⅲ的影响

目的观察氧化苦参碱对慢性肾脏病(CKD)患者血清转化生长因子-β1(TGF-β1)、Ⅲ型胶原(ColⅢ)的影响,探讨其在肾间质纤维化发生发展过程中的作用和治疗价值。方法选取健康对照者10例、慢性肾脏病患者40例,根据肾小球滤过率(GFR)分为轻度肾损害组(GFR<90 mL/min.1.73 m2,CKDⅠ)、中重度肾损害组(GFR<60 mL/min.1.73 m2,CKDⅡ),再分层随机抽样,分为常规治疗组和氧化苦参碱组。利用双抗体夹心酶联免疫吸附法分别检测各研究对象治疗前、后血清TGF-β1、ColⅢ含量,采用t检验比较两组TGF-β1、ColⅢ水平的差异。结果氧化苦参碱组TGF-β1、ColⅢ含量显著低于常规治疗组(P<0.05)。结论氧化苦参碱可能通过下调TGF-β1、ColⅢ水平,减少ECM沉积,从而达到防止肾间质纤维化的作用。     

The role of interleukin-8 produced by tumor induced fibroblasts in the development of cutaneous melanoma

Objective To determine the role of interleukin-8 (IL-8) produced by tumor induced fibroblasts in the development of cutaneous melanoma. Methods B16 melanoma cells induced L929 fibroblasts phenotype was transdifferentiated to myofibroblasts (MF) by co-culture in vitro. MF was monitored by morphology and immunophenotype for a-SMA. The level of IL-8 was detected by ELISA. The effect on B16 cell proliferation rate was estimated using MIT method in vitro. Melanoma implanting model was constructed in C57 mice. Results L929 MF phenotype could be modulated by B16 melanoma cells-derived transforming growth factor-β1 (TGF-β1) and elevated the levels of IL-8. L929 MF did not influence the B16 melanoma cells viability in vitro, but shortened the time of tumor formation and increased the incidence rates of tumors in C57 implanting model mice. Conclusion Fibroblasts can be activated by tumor cells and produce IL-8, which acts as an inflammatory cytokine promoting the development of cutaneous melanoma.     

南京长江第四大桥首级控制网的建立

介绍了南京长江四桥首级施工控制网的建立及特点．对如何采用当前测绘新技术布测特大型桥梁首级控制网并提高其成果精度、可靠性等相关技术指标进行了探讨,可供类似精密工程项目参考。     

体外大鼠成骨细胞对破骨细胞形成的影响

目的通过将鼠成骨细胞与破骨细胞直接共培养的实验方法,研究成骨细胞对破骨细胞分化及功能成熟的影响。方法按照M-CSF 30μg/L、RANKL 50μg/L的浓度对鼠骨髓单个核细胞诱导培养6 d,与原代培养3 d的鼠成骨细胞按细胞数量1∶1直接共培养,加入1,25-(OH)2D31×10-8mol/L和PGE21×10-6mol/L,利用形态学观察、TRAP染色、骨吸收陷窝检测等方法对共培养细胞进行鉴定。结果成骨细胞与破骨细胞共培养时,成骨细胞有明显的生长优势;染色后显微镜下可见大量呈单层排列的成骨细胞,偶见TRAP( )的破骨细胞。结论成骨细胞对破骨细胞分化及功能成熟的影响与两者的相对数量有关。本实验中,由于成骨细胞快速生长,当其数量多于破骨细胞时,可能使1,25-(OH)2D3对成骨细胞RANKL表达的上调作用不足,且PGE2对成骨细胞OPG表达的下调作用不足,从而主要表现为对破骨细胞形成及分化的抑制作用。     

基于顾客忠诚度的品牌价值研究

以问卷调查法和因子分析法为基础,探讨了品牌价值和顾客忠诚度的关系,认为顾客忠诚度的提高能够有效提升企业的品牌价值;并提出了基于顾客忠诚度的品牌价值提升策略。     

宫颈癌放疗患者生活质量评价

目的　探讨中晚期宫颈癌放疗患者生活质量变化及影响因素 ,评估疾病和治疗对患者生活质量的影响。方法　用生活质量问卷 (EORTCQLQ C30 )及其他调查表等对 132例宫颈癌放疗患者进行评定。结果　治疗期间总体健康状况为疗中 >疗前 >疗后。方差分析提示疼痛、呼吸困难和腹泻指标在放疗过程中有显著性变化 (P <0 .0 5 )。多因素分析表明放疗前影响患者生活质量的因素为疼痛和抑郁 ,放疗中为恶心呕吐 ,放疗后为社会功能和经济困难。结论　宫颈癌放疗患者生活质量受多种因素影响 ,放疗过程中辅助有效的对症处理和干预措施对提高生活质量具有重要临床意义。     

浅议高速公路沥青混凝土桥面排水的设计与施工

高速公路通车运营后产生的桥面损坏是沥青路面早期损坏的现象之一,结合沥青混凝土桥面施工过程中所取得的部分经验与教训,从设计和施工角度对沥青混凝土桥面结构排水、防水提出了一些方法和措施,以期提高沥青混凝土桥面的耐久性。     

气囊内模制作的空心板梁裂缝分析及措施

论述先张法气囊内模制作的空心板梁裂缝产生的机理及成因,并用有限元方法对温度应力作重点分析。根据观察、分析和计算提出控制及补救措施,取得良好效果。     

体外预应力技术在牛首山河大桥加固中的应用

体外预应力技术是加固既有钢筋混凝土桥梁结构最有效的方法之一,本文结合南京机场高速牛首山河大桥体外预应力加固工程实例,介绍了这一技术的设计原则、施工工艺及其效果评价、检验方法等。     

A DISPATCHER TRAINING SIMULATOR SYSTEM INTEGRATED WITH THE EXIST SCADA／EMS

To utilize exist SCADA(Supervisory Control and Data Acquisition)/EMS (Energy Management System) fully and economize capital, Henan Electric Power Dispatching and Communication Center in China established a set of Henan Dispatcher Training Simulator (HNDTS) base on its exist SCADA/EMS. In order to integrated with exist SCADA/EMS, the integration method and technique are proposed. Graph data integration discussed with emphasis.After integration implemented, HNDTS can share all data with SCADA/EMS and dispatchers can be trained in same environment as real work situation, in the same time it can avoid amout of work of maintenance engineers. Both advantages and disadvantages of integration are analyzed. In the end of paper, the requirement for future DTS is put forward base on the experience of author.