β淀粉样肽1～42单克隆抗体的制备

目的　制备稳定分泌β -淀粉样肽 1～ 4 2 (Aβ1-42 )单克隆抗体的杂交瘤细胞 ,产生高效价抗体。方法　通过基因工程技术 ,将Aβ1-42 基因融合于乙肝核心抗原 (HBcAg)的主要免疫优势区 (MIR) ,制备Aβ和HBcAg的融合蛋白 ,免疫BALB/c小鼠 ,取脾细胞与SP2 / 0细胞融合 ,筛选能稳定分泌Aβ1-42 单克隆抗体的杂交瘤细胞。结果　得到两株能稳定分泌Aβ1-42 单克隆抗体的杂交瘤细胞 ,Aβ1-42 单克隆抗体的Ig亚类为IgG3 。结论　制备得到的Aβ1-42 单克隆抗体特异性强 ,效价高     

THE GENE EXPRESSION OF BDNF IN NORMAL RABBIT RETINA

Objective To investigate the distribution of brain-derived neurotrophic factor(BDNF) protein in the rabbit retina. Methods Immune response material in the retina was observed using BDNF antibody by the method of immunohistochemistry. Results BDNF gene expression was mainly found in the RGCs, also in innernuclei cells and outernuclei cells in rabbit retina. Conclusion RGC is not only the target cell of BDNF, but also express the BDNF protein. BDNF from multi-sources participates in the regulation of RGCs.     

Effects of Heat Intensity and Inflow Wind on the Reactive Pollution Dispersion in Urban Street Canyon

This paper investigates the impacts of heating intensity and inflow wind speed on the characteristics of reactive pollutant dispersion in street canyons using the computational fluid dynamic (CFD) model that includes the transportation of NO, NO₂, and O₃ coupled with NO-NO₂-O₃ photochemistry. The results indicated that the heat intensity and inflow wind speed have a significant influence on the flow field, temperature field and the characteristics of reactive pollutant dispersion in and above the street canyon. With the street canyon bottom heating intensity increasing, NO, NO₂ and O₃ concentrations in street canyon are decreased. The O₃ concentration reductions are even more than the NO and NO₂ concentrations. Improving the inflow wind speed can significantly reduce the NO and NO₂ concentrations within street canyons. But the O₃ concentrations have a slight rise with wind speed increasing. The results would be useful for understanding the interrelation among reactive vehicle emissions, and provide references for urban planners.     

大连市常规公交规划思路探讨

城市常规公交系统建设是贯彻公交优先、促进高质量发展的关键.本文从分析大连市常规公交发展现状和面临问题入手,结合城市交通发展趋势,提出常规公交发展目标,在此基础上探讨了新时期常规公交规划要从公交网络、规划目标、服务模式、技术方法等四方面转变思路,强化两网融合和以人为本的思想,增强常规公交的吸引力,促进城市高质量发展.     

分水岭隧道溶洞及隧道拱顶下沉的治理

介绍了分水岭隧道洞身k25 196～ 230地层地质情况,以及隧道开挖后出现的病害,从设计和施工上提出了根治隧道溶洞拱顶下沉、隧道拱脚位移的措施。     

长沙水路集装箱运输竞争力浅析

介绍了水运与其它运输方式相比所具有的优势,集装箱运输与散货、件杂货运输相比所占的比重,阐述了长沙集装箱码头升级改造,进出口岸优势,长沙外贸经济的发展势头,以及参与企业的整体实力等有利因素,并分析了影响竞争力的不利因素。     

低空空域飞行冲突避让算法

为了预防航空器在低空空域飞行中发生相撞, 根据空中交通管理规则的飞行间隔规定, 采用速度矢量三角分析法, 分析了避免冲突的基本条件, 确定了航空器B与航空器A的速度矢量关系, 提出了调整速度和改变航向两种解决方法, 推导出速度和航向改变量的计算公式。通过系统演算和DRS-98型雷达管制模拟机的验证表明, 在任何情况下都能够使用改航法避让飞行冲突, 航空器之间的距离和航向差越大, 航向的改变量就越小; 当航空器之间的距离大于6倍的飞行间隔时, 应当使用调速法避让飞行冲突, 这样航空器可以继续保持原定的飞行航线。     

区域运输通道内客运方式分担率模型

鉴于Wardrop原理假设通道内的旅客对各种运输方式的实际出行费用能够完全准确估计的不足, 利用不确定规划理论, 结合不同出行距离、不同收入水平的旅客对运输方式服务属性的评价, 用数学期望表示旅客出行的广义费用, 提出了不确定条件下运输通道内各种运输方式旅客最优和运输系统最优客运量分担率计算模型, 以及多目标客运量分担率计算模型, 并设计了用于求解模型的基于随机模拟的遗传算法。客运量分担率的预测结果与实际测量值之间平均误差为8.13%, 说明本模型能够有效地模拟旅客在出行时对运输方式选择的不确定性。     

谈预应力空心板反拱、翘曲现象在施工中的控制

为有效控制先张法预应力空心板反拱、翘曲现象,应首先控制张拉台座质量、同时在钢绞线张拉与放张、钢筋骨架的绑扎、模板的支立、混凝土浇筑、混凝土养护、梁板的存放的一系列环节中进行控制.     

STUDY ON THE THERAPEUTIC EFFECTS OF GINSENOSIDE Rg-1 AND GASTRODINE ON AD MODEL RATS INDUCED BY β-AMYLOID PEPTIDE （25-35）

Alzheimer sdisease(AD)isadegenerativedis orderofcentralnervoussysteminhumanbeing.It ischaracterizedbylossofmemory,cognitivede clineandmisbehavior,aswellasoxidativedamage isincreasedandmetabolicrateisdecreased.Major pathologicalmarksofADareextracellarsenil…