我国汽车产业发展影响因素研究

通过S-C-P分析和环境分析研究得出我国汽车产业发展的主要影响因素,分别就这些因素对我国汽车产业发展的影响进行比较深入的研究,通过相关性分析、单位根检验和协整检验进行实证分析。     

Travel time estimation oriented freeway sensor placement problem considering sensor failure

Optimal sensor placement on freeway corridor is of great interest to transportation authorities. However, current traffic sensors are easily subject to various failures. Therefore, it is necessary to incorporate sensor failure into the optimal sensor placement model. In this article, a two-stage stochastic model is proposed for the purpose of travel time estimation on freeway corridor. To balance the effectiveness and reliability, a stochastic conditional value at risk (CVaR) model is also proposed. Since both models are too complicated, a customized genetic algorithm is developed. Numerical experiments show that considering sensor failure makes a significant performance improvement in the sensor placement pattern. Sensitivity analysis is also applied to investigate the impact of a number of allowable sensors and different traffic sensor failure probability.     

用于车身制造质量控制的白车身测点布置及优化

在对检测点进行分类定义后阐述了车身设计阶段各类测点的布置原则，在此基础上提出了面向制造偏差诊断的测点布置位置优化方法。     

汽车车速巡航模糊控制

汽车巡航控制系统是一种汽车辅助驾驶系统，可以在40～200km／h的车速范围内，启动该系统，人为设定一个车速，驾驶员将不需再操控油门，系统即可自动控制汽车恒速行驶。由于汽车巡航控制系统的强非线性，以及受外界负荷的扰动、复杂的运行工况等因素影响，该系统较适合采用模糊自整定HD控制方法，以保证系统具有良好的动态稳定性。     

城市道路景观环境建设的探讨

城市道路景观环境已越来越引起社会的关注。城市居民既希望有便捷、安全的交通 ,同时希望有一个安全、优美、贴近自然的交通环境。本文重点对建设城市道路景观环境的原则进行探讨     

三维植被网路基边坡防护

三维植被网作为一种新型的坡面防护材料 ,具有很好的使用性能和推广价值。本文详细介绍其特点、优点以及施工工艺。并给出运用实例     

辽宁省东部山区公路边沟设计降雨重现期的确定

本文分析了现行公路排水规范在设计降雨重现期方面规定的不足之处,结合辽宁省东部山区的实际降雨资料,经过计算和分析,得出了符合辽宁省东部山区公路自身特点的设计降雨重现期.     

多房棘球绦虫重组Bb-Em14-3-3疫苗的构建及其表达效率

目的构建多房棘球绦虫(Em)重组双歧杆菌(Bb)-Em14-3-3疫苗,并研究Em14-3-3分子在大肠埃希菌BL21(DE3)中的表达效率。方法通过PCR扩增Em14-3-3抗原编码基因;将该基因定向克隆于含有谷胱甘肽-S-转移酶(GST)基因的大肠埃希菌-双歧杆菌穿梭表达载体pGEX-1λT,构建重组质粒pGEX-Em14-3-3;用电穿孔法将该质粒导入Bb,构建多房棘球绦虫重组Bb-Em14-3-3疫苗。经PCR和酶切鉴定后以IPTG诱导表达Em14-3-3/GST融合蛋白;SDS-PAGE及Western blot对表达产物进行鉴定。结果PCR成功扩增出530 bp的Em14-3-3基因;双酶切证实Em14-3-3基因插入pGEX-1λT中;PCR证实重组Bb-Em14-3-3疫苗构建成功;SDS-PAGE及Western blot分析显示重组质粒转化宿主菌在IPTG诱导下高效表达了Em14-3-3/GST融合蛋白,表达效率为23%。结论成功构建了多房棘球绦虫重组Bb-Em14-3-3疫苗,重组质粒pGEX-Em14-3-3在大肠杆菌中获得了高效表达,Western blot结果提示表达出的Em14-3-3重组蛋白具有特异抗原性。     

AQP4在COPD支气管黏膜的表达及其与患者肺功能、气道黏膜炎症的相关性

目的通过免疫组化法检测支气管黏膜中水通道蛋白-4(aquaporin-4,AQP4)的表达,观察正常组和慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)组支气管黏膜中AQP4表达与气道黏膜炎症及肺功能的关系,明确AQP4在COPD发生、发展中的作用,为COPD的防治开辟新途径。方法选择正常人和COPD患者各30例,测试肺功能,并行支气管镜检查,取得支气管黏膜标本,用免疫组化法检测AQP4表达,HE染色观察支气管黏膜炎症细胞浸润程度。结果COPD组较对照组AQP4表达减弱有极显著差异(P<0.001)。AQP4表达和FEV1/FVC、FEV1/pred%呈正相关(P<0.001),与支气管黏膜炎症严重程度呈负相关(P<0.001)。结论COPD患者AQP4表达减少可能是其肺功能下降及气道炎症加重的原因之一。临床上可以通过改善AQP4治疗COPD。     

A total generalized optimal velocity model and its numerical tests

A car-following model named total generalized optimal velocity model （TGOVM） was developed with a consideration of an arbitrary number of preceding vehicles before current one based on analyzing the previous models such as optimal velocity model （OVM）, generalized OVM （GOVM） and improved GOVM （IGOVM）. This model describes the physical phenomena of traffic flow more exactly and realistically than previous models. Also the performance of this model was checked out by simulating the acceleration and deceleration process for a small delay time. On a single circular lane, the evolution of the traffic congestion was studied for a different number of headways and relative velocities of the preceding vehicles being taken into account. The simulation results show that TGOVM is reasonable and correct.