水泥稳定风积砂作为路面基层材料的试验研究

采用均匀设计的方法来优化试验,以水泥、碎石、风积砂为原材料进行试验研究,得到水泥稳定风积砂的最佳含水量、最大干密度、无侧限抗压强度、回弹模量等指标,并通过SPSS软件进行回归分析,建立回归方程.研究结果表明水泥用量对无侧限抗压强度的影响最大,其次是碎石的用量,风积砂的用量对强度的影响较小.通过铺筑试验路的试验和观测,结果表明,水泥稳定风积砂基层状况良好,且弯沉值满足有关的要求.     

CLONING SEGMENT SPIKE PROTEIN GENE OF SARS-COV AND ITSEXPRESSION IN ESCHERICHIA COLI

Objective Expressing and purifying the seglnent of SARS-CoV spike protein in E. Coli Methods The target gene was obtained by RT-PCR. The PCR product was cloned into pEGM- T Easy Vector, sequencing and double restriction digestion (BamH Ⅰ , Pst Ⅰ) were performed. The target gene was subcloned into PQE30 expression vector. The gene was expressed in the E. coli strain M15 cells induced by IPTG. The protein was purified with a nickel HiTrap chelating metal affinity column. Results The recombinant expression plasmid was successfully constructed and the protein was well expressed in E. coli strain M15 cells. The ideal pure protein was obtained by purification. Western blotting analysis suggested the protein could act with the convalescent sera of lab confirmed SARS patients. Conclusion The segment of SARS-CoV spike protein was well expressed and purified, and can be applied in diagnosis and immunological research of SARS.     

基于MATLAB的增压发动机动态排放仿真模型研究

介绍了研究增压发动机动态排放问题的意义以及研究方法.采用了增压发动机动态性能模拟与稳态试验相结合的方法,建立了涡轮增压柴油机的准线性动态模型,利用稳态试验数据建立神经网络排放模型,提出了一种将2种模型结合起来在MATLAB/SIMULINK环境下的增压发动机动态排放预测仿真模型.     

桥梁现场常见病害及检查

建于上世纪60、70年代的公路桥梁,现在大多带病服务于现代交通运输中,因此,对这些桥梁进行病害检查就显得十分重要。桥面系、上部构造、支座、墩台及基础是病害检查的重要部位。文中指出了这些部位的常见病害,并分析了产生这些病害的原因,为桥梁的养护决策提供依据。     

大源渡枢纽工程河中纵向围堰施工技术评述

对大源渡枢纽工程河中纵向围堰施工技术进行了评述。从施工方案的比较，混凝土搅拌站定位，混凝土生产工艺，材料输送和施工工艺等方面进行了介绍与分析，并对施工中存在的质量问题提出了灌浆等解决措施。     

葡萄糖、胰岛素和尿酸对猪近端肾小管上皮细胞株血红素氧合酶-1的影响

目的观察不同浓度葡萄糖、胰岛素和尿酸的培养条件下猪近端肾小管上皮细胞株(LLC-PK1)血红素氧合酶(HO-1)活性的改变,以探讨代谢相关因素在细胞水平对肾小管上皮细胞的影响。方法以LLC-PK1为研究对象,观察葡萄糖浓度为5、10、22、33 mmol/L,胰岛素浓度为0、10-9、10-8、10-7mol/L,尿酸浓度为0、0.1、0.2、0.4 mmol/L培养48 h条件下,LLC-PK1细胞HO-1活性的改变。结果0.1 mmol/L0、.2 mmol/L和0.4 mmol/L尿酸刺激48 h后,LLC-PK1的HO-1活性增加,并呈剂量依赖关系,而上述浓度的葡萄糖和胰岛素刺激48 h对LLC-PK1的HO-1活性无影响。结论一定浓度的尿酸可使LLC-PK1的HO-1活性升高,提示尿酸在细胞水平对肾小管上皮细胞的氧化应激过程产生影响。     

单壁钢围堰在大潮差桥梁桩基加固中的应用

桥梁水中桩基在江、河、海水的日益冲刷下出现混凝土剥落、钢筋外露锈蚀,导致桩基缩径,极大地威胁着桥梁结构的安全性,这就需要对桩基进行维修加固。结合《大潮差桥梁桩基钢围堰施工技术研究》(S20130013)和温州市东瓯大桥桩基加固工程实例,介绍单壁钢围堰技术在处理水下桩基础破损露筋等质量问题中的应用,阐述了钢围堰加固桩基施工方案的实施和施工中应注意的几个关键技术。     

双浮体波浪能装置的振荡和能量转换特性研究（英文）

In this study, we investigated the hydrodynamic and energy conversion performance of a double-float wave energy converter(WEC) based on the linear theory of water waves. The generator power take-off(PTO) system is modeled as a combination of a linear viscous damping and a linear spring. Using the frequency domain method, the optimal damping coefficient of the generator PTO system is derived to achieve the optimal conversion efficiency(capture width ratio).Based on the potential flow theory and the higher-order boundary element method(HOBEM), we constructed a threedimensional model of double-float WEC to study its hydrodynamic performance and response in the time domain. Only the heave motion of the two-body system is considered and a virtual function is introduced to decouple the motions of the floats. The energy conversion character of the double-float WEC is also evaluated. The investigation is carried out over a wide range of incident wave frequency. By analyzing the effects of the incident wave frequency, we derive the PTO's damping coefficient for the double-float WEC's capture width ratio and the relationships between the capture width ratio and the natural frequencies of the lower and upper floats. In addition, it is capable to modify the natural frequencies of the two floats by changing the stiffness coefficients of the PTO and mooring systems. We found that the natural frequencies of the device can directly influence the peak frequency of the capture width, which may provide an important reference for the design of WECs.     

短消息业务的发展与需求

介绍了短消息业务的发展、短消息业务所具有的独特优势、短消息业务的发展方向、短消息业务的应用类型及发展短消息业务的需求.     

公路无伸缩缝桥梁应用探讨

针对目前桥梁中应用整体式、半整体式桥台无缝桥梁,弹塑体(沥青碎石)伸缩缝暗埋式无缝桥梁、全桥桥面连续无缝桥梁无缝技术就其原理、适用范围、施工工艺等问题进行详细的阐述,并重点阐述了全桥桥面连续结构桥梁的适用性和注意事项。