警民联合推行“五三二一安全行车法”斑马线前更安全

正为进一步推进文明交通行动,树立安全行车理念,提高广大公交车驾驶员、私家车主及市民文明礼让、文明交通意识,3月14日,交运温馨巴士联合青岛交警,在深圳路尚东区公交站附近斑马线旁,开展五三二一安全行车法主题活动。近50名参与此活动的公交车和私家车驾驶员代表纷纷表示,将以身作则,率先做好文明礼让、文明交通。     

White Layer of Hard Turned Surface by Sharp CBN Tool

IntroductionHard turning has been recognized as one of themost competitive clean machining technologies inreplacing of abrasive-based processes, with the de-velopment of high precision numerical machinetools and super-hardness tool material such as ce-ramic, CBN/PCBN (Cubic Boron Nitride/Poly-crystalline Cubic Boron Nitride)[1,2]. Hard turningoperation has shown geometrical flexibility, eco-nomics advantage, environmental friendliness, andsuperior surface integrity which is usually define…     

IAA与HRP联合作用对人胰腺癌BXPC-3的细胞毒效应

目的　研究吲哚乙酸(IAA)与辣根过氧化物酶(HRP)在体外共同作用于人胰腺癌BXPC 3 细胞所产生的细胞毒效应。方法　MTT法测定IAA/HRP对BXPC 3细胞增殖的影响;流式细胞仪分析BXPC 3 细胞周期时相变化;胎盘兰(曲利本兰)排斥试验,研究IAA/HRP对BXPC 3 细胞死亡率的影响;原位细胞凋亡检测试剂盒检测IAA/HRP作用后BXPC 3细胞的凋亡。结果　IAA/HRP体外对BXPC 3 细胞有抑制生长的效应,且这种效应有浓度和时间依赖性;BXPC 3细胞在IAA/HRP作用48 h后细胞周期被阻滞在G1期和G2期;IAA/HRP作用72 h后对照组和药物组的凋亡细胞数存在着显著性差异,且凋亡细胞数有浓度依赖性。结论　IAA/HRP对胰腺癌BXPC 3 细胞有细胞毒作用,可体外抑制细胞的增殖,诱导凋亡。     

萘乙酸抑制K562细胞增殖作用的初步研究

目的研究萘乙酸(NAA)对K562细胞增殖的抑制作用。方法应用MTT法、细胞计数法、流式细胞术、分裂指数分析、tunnel法以及姊妹染色单体交换(sister chromatid exchange,SCE)和微核制备,对NAA处理的K562细胞进行细胞增殖、细胞周期、凋亡以及染色体损伤检测。结果与对照组相比,NAA可以有效抑制K562细胞的生长,并将细胞阻滞在G1期,降低细胞分裂指数,促进细胞凋亡,提高K562细胞SCE频率以及微核形成率。结论NAA具有抑制K562细胞增殖的作用。     

芳玻韧布加固楚大高速公路K66＋950桥荷载试验成果分析

通过对采用芳纶／玻纤——“芳玻韧布”复合材料及桥面拆除重浇混凝土等加固的楚大高速公路K066＋950桥荷载对比试验，测试成果分析表明，加固后桥梁的整体工作性能得到提高，其实测挠度减小了30％左右，桥梁可以正常营运，从而进一步证实芳玻韧布在桥梁加固应用中的有效性和普遍实用性。     

公路线形设计体会

介绍了在线路设计时,设计工作者如何结合司机心理和汽车行驶力学要求特点,处理好直线与曲线,平面与纵断面的关系,使之逐步达到具有三维空间立体线形的视觉好、顺畅的良好的线形,行驶舒适的目的。     

青岛口岸外贸集装箱出口通关模式研究

现代物流已成为发达国家国民经济的动脉和基础产业,物流人才直接影响到企业生存和发展。物流人才培养是一个系统工程,涉及到政府、教育部门、高等院校、职业院校、企业和社会培训机构。本文在调查研究的基础上,探讨了物流人才培养体系的构建和培养模式的选择。     

石板坡大桥钢箱梁车载作用下局部应力分析

石板坡大桥主跨由钢箱梁与预应力混凝土箱梁组成。针对该桥钢箱梁桥面板局部受力复杂的情况，运用ANSYS软件建立了钢箱梁结构分析的空间有限元模型，分析了钢箱梁在车载作用下局部应力的分布特点。     

Study of the mechanism on the apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase

Objective To investigate the mechanisms of apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase. Methods Human leukemia cell line K562 were exposed to indole-3-acetic acid （IAA） at 20, 40, 60, 80 or 100 mol/L and horseradish peroxidase（HRP） at 1.2 g/mL for varying times. MTT assay was applied to detect the cell proliferation. Flow cytometry was performed to detect the arrest of cell cycle. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） assay was used to measure apoptosis. 2, 7-dichlorofluorescin diacetate （DCFH-DA） uptake was measured to determine free radical by confocal microscope. Content of malondiadehyde （MDA） and activity of superoxide dismutase （SOD） were measured by biochemical methods. Results IAA/HRP initiated growth inhibition of K562 cells in a dose- and time-dependent manner. Flow cytometry revealed that cell cycle arrested at G1/G0 after 24 hours treatment. After 72 hours treatment, apoptotic rate of 100 mol/L IAA group increased to 43.9 %, which was 5 times that of control（P〈0.01）. Content of MDA and activity of SOD increased respectively in treatments compared with control. Meanwhile, IAA/HRP stimulated the formation of free radical, which was increased by IAA concentration-dependently. Conclusion The combination of IAAand HRP can inhibit the growth of Human leukemia cell line K562 in vitro by inducing apoptosis which is associated with the increase of free radical. The combination of IAA and HRP might be a promising chemopreventive and chemotherapeutic agent against human leukemia.     

Study and Development of the Biped Ice Skating Robot with Passive Wheels

A new passive wheel type of biped ice-skating robot(BISR)which was able to imitate human skating motion was developed. Firstly, the characteristics of two types of human skating gait were introduced; secondly, after simplifying the kinematical model, the BISR's motion principle was presented; then the construction and control system of BISR were proposed; at last, the skating experiment of the BISR in a symmetric gait mode was conducted and some conclusions were drawn.