城市客运模式选择研究

国内目前各城市的客运发展存在着地域之间不平衡,发展面临问题众多的局面,行业管理存在较多待破解的关键性问题.本文从城市客运管理运营模式作为研究视角,通过审视和比较世界不同城市交通的发展模式,结合影响城市客运模式的主要决定因素,论证国内目前的发展阶段和城市客运模式,研究提出适合于中国特点的、可持续发展的城市交通发展模式.     

小城镇道路交通安全管理规划研究

通过分析我国小城城镇特点及其交通安全面临的问题,并结合交通安全规划的理论,提出小城镇道路交通安全规划的具体原则和内容,具有一定的理论和实际意义。     

Case法高应变桩基承载力检测的理论与方法

Case法高应变桩基承载力检测,与静载法相比具有简便、经济的优点。它是运用一维杆弹性波理论,通过在桩顶施加锤击力,使其达到瞬态高应力应变状态,从而确定桩的极限承载力的。对case法的理论和试验方法进行了介绍。     

公路线形设计体会

介绍了在线路设计时,设计工作者如何结合司机心理和汽车行驶力学要求特点,处理好直线与曲线,平面与纵断面的关系,使之逐步达到具有三维空间立体线形的视觉好、顺畅的良好的线形,行驶舒适的目的。     

城市道路安全审计探讨

分析了我国城市道路在安全方面存在的问题,结合城市道路的特点,探讨了城市道路实行安全审计的细则及城市道路安全审计的重要技术问题和采取的减少交通事故应对措施,以减少城市道路交通事故和消减潜在的安全隐患。     

石板坡大桥钢箱梁车载作用下局部应力分析

石板坡大桥主跨由钢箱梁与预应力混凝土箱梁组成。针对该桥钢箱梁桥面板局部受力复杂的情况，运用ANSYS软件建立了钢箱梁结构分析的空间有限元模型，分析了钢箱梁在车载作用下局部应力的分布特点。     

青岛口岸外贸集装箱出口通关模式研究

现代物流已成为发达国家国民经济的动脉和基础产业,物流人才直接影响到企业生存和发展。物流人才培养是一个系统工程,涉及到政府、教育部门、高等院校、职业院校、企业和社会培训机构。本文在调查研究的基础上,探讨了物流人才培养体系的构建和培养模式的选择。     

Study of the mechanism on the apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase

Objective To investigate the mechanisms of apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase. Methods Human leukemia cell line K562 were exposed to indole-3-acetic acid （IAA） at 20, 40, 60, 80 or 100 mol/L and horseradish peroxidase（HRP） at 1.2 g/mL for varying times. MTT assay was applied to detect the cell proliferation. Flow cytometry was performed to detect the arrest of cell cycle. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） assay was used to measure apoptosis. 2, 7-dichlorofluorescin diacetate （DCFH-DA） uptake was measured to determine free radical by confocal microscope. Content of malondiadehyde （MDA） and activity of superoxide dismutase （SOD） were measured by biochemical methods. Results IAA/HRP initiated growth inhibition of K562 cells in a dose- and time-dependent manner. Flow cytometry revealed that cell cycle arrested at G1/G0 after 24 hours treatment. After 72 hours treatment, apoptotic rate of 100 mol/L IAA group increased to 43.9 %, which was 5 times that of control（P〈0.01）. Content of MDA and activity of SOD increased respectively in treatments compared with control. Meanwhile, IAA/HRP stimulated the formation of free radical, which was increased by IAA concentration-dependently. Conclusion The combination of IAAand HRP can inhibit the growth of Human leukemia cell line K562 in vitro by inducing apoptosis which is associated with the increase of free radical. The combination of IAA and HRP might be a promising chemopreventive and chemotherapeutic agent against human leukemia.     

Study and Development of the Biped Ice Skating Robot with Passive Wheels

A new passive wheel type of biped ice-skating robot(BISR)which was able to imitate human skating motion was developed. Firstly, the characteristics of two types of human skating gait were introduced; secondly, after simplifying the kinematical model, the BISR's motion principle was presented; then the construction and control system of BISR were proposed; at last, the skating experiment of the BISR in a symmetric gait mode was conducted and some conclusions were drawn.     

警民联合推行“五三二一安全行车法”斑马线前更安全

正为进一步推进文明交通行动,树立安全行车理念,提高广大公交车驾驶员、私家车主及市民文明礼让、文明交通意识,3月14日,交运温馨巴士联合青岛交警,在深圳路尚东区公交站附近斑马线旁,开展五三二一安全行车法主题活动。近50名参与此活动的公交车和私家车驾驶员代表纷纷表示,将以身作则,率先做好文明礼让、文明交通。