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在物流活动的商品配送运输中，由于受不确定因素的干扰，对配送车辆调度的适时性造成了很大影响，从而增加了配送成本和降低了配送的服务质量。本文考虑客户对配送时间的要求和车辆行驶时间的不确定性，建立了以车辆配送总行驶距离最小化为目标的机会约束规划模型，并构造了求解该模型的单亲遗传算法，通过实例对模型求解，结果表明，该算法是很有效的。     

谈谈课堂教学

系作者对学校青年教师所作关于教学方法系列讲座之一,作者结合三十余年的教学实践对如何组织课堂教学漫谈自己的心得体会.强调了课堂教学中知识传授和能力培养两大功能的有机结合,教师的主导作用就是要时时处处体现把能力培养渗透到知识传授的全过程中去.     

谈谈课外辅导

系作者对学校青年教师所作关于教学方法系列讲座之二,作者结合三十余年的教学实践对如何进行课外辅导和答疑漫谈自己的心得体会.强调了课外辅导和答疑对学生接受知识和能力培养的重要意义.     

应力波在基桩检测中的应用

应力波在桩基检测中的应用及各种因素对其影响。     

平阳霉素局部注射对鼻息肉病息肉组织病理学的影响

目的通过观察平阳霉素治疗前后鼻息肉病息肉组织的病理变化情况,探讨平阳霉素治疗鼻息肉病的机制。方法应用末端脱氧核苷酸转移酶介导的脱氧三磷酸尿苷缺口末端标记法(TUNEL法)检测凋亡细胞,与相邻连续的HE染色片对位对照观察嗜酸性粒细胞的凋亡情况。结果平阳霉素治疗前鼻息肉组织嗜酸性粒细胞表达显著高于正常下鼻甲(P<0.01);治疗后鼻息肉组织中的嗜酸性粒细胞表达明显低于治疗前(P<0.01);治疗后鼻息肉组织中嗜酸性粒细胞的凋亡指数高于治疗前(P<0.01)。结论嗜酸性粒细胞是鼻息肉病息肉组织中主要的浸润炎细胞;平阳霉素可通过促进嗜酸性粒细胞凋亡达到治疗鼻息肉病的目的。     

Study of the mechanism on the apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase

Objective To investigate the mechanisms of apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase. Methods Human leukemia cell line K562 were exposed to indole-3-acetic acid （IAA） at 20, 40, 60, 80 or 100 mol/L and horseradish peroxidase（HRP） at 1.2 g/mL for varying times. MTT assay was applied to detect the cell proliferation. Flow cytometry was performed to detect the arrest of cell cycle. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） assay was used to measure apoptosis. 2, 7-dichlorofluorescin diacetate （DCFH-DA） uptake was measured to determine free radical by confocal microscope. Content of malondiadehyde （MDA） and activity of superoxide dismutase （SOD） were measured by biochemical methods. Results IAA/HRP initiated growth inhibition of K562 cells in a dose- and time-dependent manner. Flow cytometry revealed that cell cycle arrested at G1/G0 after 24 hours treatment. After 72 hours treatment, apoptotic rate of 100 mol/L IAA group increased to 43.9 %, which was 5 times that of control（P〈0.01）. Content of MDA and activity of SOD increased respectively in treatments compared with control. Meanwhile, IAA/HRP stimulated the formation of free radical, which was increased by IAA concentration-dependently. Conclusion The combination of IAAand HRP can inhibit the growth of Human leukemia cell line K562 in vitro by inducing apoptosis which is associated with the increase of free radical. The combination of IAA and HRP might be a promising chemopreventive and chemotherapeutic agent against human leukemia.     

水泥砼路面错台快速修补材料的研究

采用正交设计的方法对快硬硫铝酸盐水泥和聚合物丁苯乳液进行配合比试验,确定出最佳水泥砼配合比,通过路用性能对比试验和耐久性对比试验表明,这种有机、无机多相复合的新型道路水泥砼能有效的弥补普通砼的缺点,具有优良的力学和路用性能:早期强度高,养护12 h即可开放交通,干缩小,耐久性能好,与旧路面之间层间黏结强度高,并且在错台高度仅为0.6 cm时,模拟错台修补效果很好,没有出现脱落和开裂.     

现代有轨电车安全运行的危险源识别与风险评估

随着现代有轨电车的快速发展,运行安全问题日益凸显。针对现代有轨电车运行安全问题,从系统外部和系统内部分析其安全运行危险因素,建立危险因素库。运用事故树法,根据各个基本事件的逻辑关系搭建事故树,识别危险源,确定事故发生原因。基于LEC法赋值3种因素指标,进行风险评估,确定风险等级。分析结果表明交通环境差、机非混行、乘客的不安全行为以及驾驶员操作不当是造成事故发生的主要危险源。自然灾害、驾驶员操作不当以及共享路权导致事故后果严重,风险等级高。根据结果提出相应的风险防控措施,保证车辆的安全运行。     

滑框倒模技术在空心薄壁高墩中的应用

介绍了京福高速公路福建段上洋大桥空心薄壁高墩中采用的滑框倒模技术,该技术结合了滑模和翻模的优点,在保证工程施工进度、质量的同时,还较好地满足了墩身外观质量的要求.根据滑框倒模的施工特点和施工效果,对这一技术提出了其应用前景.     

EXPRESSION OF NESTIN AND GLIAL FIBRILLARY ACIDIC PROTEIN IN DIFFERENT PERIOD AFTER SPINAL INJURY IN ADULT RATS

Objective To study the expression of Nestin and glial fibrillary acidic protein (GFAP) in different period after spinal injury in adult rats. Methods Animal moels were created by artery clamp. Expression of Nestin and GFAP in different period ( 1,3,5days; 1-8 weeks) and different area( injury locus and its surrounding tissue ) after spinal injury were observed pathologicaly using immunofluorescence and LeicaQ5001W imaging processing system.Results There was expression of Nestin and GFAP in injured area 1 day after injury. The expression increased not only in in injured area but its sourrounding 3--7 days later and gradually got to peak value. As the time went on, expression of Nestin and GFAP dereased. Conclusion Spinal injury can induce the expression of Nestin. Nerve stem cell has response to spinal injury. There is positive correlation between expression of Nestin and hyperplasia of reactivity astrocyte. Nerve stem cell maybe invovled in the repair of central nervous system (CNS).