THE STUDY OF MICROSATELLITES ALTERATION IN DIAGNOSES OF BLADDER CANCER

Objective To investigate the possibility of microsatellite alteration （MA） in diagnosis of bladder cancer of Chinese people, and find the better panel which will be used in clinic. Methods A total of 6 and 10 microsatellite markers were chosen, PCR-SSLP silver staining assay was performed in 31 and 32 bladder cancers tissue, exfoliate cells in urine and 10, 15 non-bladder cancers exfoliate cells in urine, respectively. Results MA （＋） was found in 28 out of 31, 30 out of 32 bladder cancers, and the sensitivity was 90. 3%, 93.7% respectively. The MA of urine sediment of 25 non-bladder cancers was negative, and the specificity was 100%. The cytology was carried out among 19 out of 31, 20 out of 32 bladder cancers at the same time, 2 cases （10.3%） and 3 cases （15%） were found cancer positive, and the sensitivity is significantly lower than that by the analysis of MA in exfoliated cells. Conclusion MA was not associated with grade and stage of the bladder cancer. MA assay is a sensitive and effective method for the early detection of bladder cancer and post-operation surveillance.     

一种改良式质粒DNA提取方法的建立与应用

目的建立一种稳定、可靠的质粒DNA提取改良方法,探讨其在分子生物学实验研究中的应用价值。方法将含有目的质粒的菌株扩增后,运用改良后的碱裂解法进行质粒DNA小量提取,微量核酸仪测定提取质粒DNA的产量及纯度;采用琼脂糖凝胶电泳、内切酶酶切及质粒转染真核细胞鉴定提取质粒DNA的质量及转染效率。结果改良式质粒提取方法获得质粒DNA产量为3.2 mg/L菌液,A260/280=1.91;内切酶酶切完全;质粒DNA以超螺旋结构为主;真核细胞转染效率为20%左右。结论改良式质粒DNA抽提方法操作简单、实用,获得质粒DNA产量多、质量高,能达到分子生物学常规实验的要求。     

REAL-TIME DETECTION OF SURVIVIN mRNA EXPRESSION IN CERVICAL CANCER CELL LINES USING MOLECULAR BEACON IMAGING

Objective To detect the expression of survivin mRNA in cervical cancer cell lines using molecular beacon imaging technology. Methods Human cervical cancer cells （HeLa and SiHa） and human fetal lung fibroblast HFL-I were cultured in vitro. After adding 100 nmol/L survivin mRNA molecular beacon, the fluorescent signals were observed under fluorescent microscope. The expressions of survivin in cervical cancer cells and HFL-I cell were examined by immunocytochemical streptravidin-biothin peroxidase （SP） assay at the same time. Results Two kinds of survivin mRNA molecular beacon, with different color fluorescence, had strong fluorescent signal in cervical cancer cell lines, and the signal in SiHa cell line was stronger, but these signals were not found in HFL-I ; Immunocytochemical staining of positive survivin was located in the cytoplasm of cervical cancer cell lines HeLa and SiHa, whereas, no expression of survivin was detected in HFL-I cell line. Conclusion The technology of molecular beacon imaging can be used to detect the expression of survivin mRNA in viable cells successfully, and may provide a new approach to the diagnosis of early stage cervical cancer and the following-up in the clinic.     

城市轻轨车辆转向架方案的选择

本文根据城市轻轨车辆用转向架的运用条件和技术要求,结合我国的技术水平和传统特点,对城市轻轨车辆转向架结构设计中应考虑的问题进行了分析,认为在进行轻轨车辆转向架设计时,应采用空气弹簧、盘形制动,弹性车轮、磨耗形踏面、焊接构架等先进技术.     

�۽�ͨϵͳһ�廯

指出了一体化交通系统是与智能交通系统并行的世界性的发展趋势，论述了交通系统一体化的必要性。提出了从三个方面理解一体化交通系统的内涵:交通系统的一体化是对交通系统资源的整体优化，交通系统的一体化是对交通需求的统一管理，交通系统的一体化体现在交通运输的全过程。指出了交通系统一体化的外延。重点讨论了两个问题:一体化交通系统与智能交通系统的关系问题，ITS与交通工程的关系问题。提出了把非技术范畴的因素从ITS中分离开来的观点。     

尘埃落定:B型引力探测器50年探索总结——B型引力探测器作为一个概念简单的实验,验证了广义相对论的两个预言,但是精度未达到预期目标

1962年,Francis Everitt来到斯坦福大学,受命成为B型引力探测器( GP- B)的首位全职雇员.历经半个世纪,耗资7.6亿美元之后,Everitt在GP-B发射升空7年之后宣布,GP-B完成了数据分析,分析结果表明,爱因斯坦的巨著又一次顺利地经受住了考验.按照实验设定的参数,GP-B将以前所未有的精度来测量爱因斯坦广义相对论的两个预言:所谓的短程线效应和坐标系拖曳效应,这两个效应会令毫无外力矩作用的绕轨飞行的陀螺仪发生进动.Everitt和他的同事最终给出了短程线效应的测量精度为0.3%,而更小的坐标系拖曳效应测量精度为20%.在GP-B历时半个世纪的实验过程中,他们制造了荣膺最完美球体吉尼斯世界记录的乒乓球大小的陀螺转子,并用5年的时间坚持不懈地对陀螺仪干扰力矩进行建模和数据处理等,堪称是一个不同凡响的艰难历程.     

一种100％低地板有轨电车转向架构架的焊接工艺

介绍了一种100%低地板有轨电车转向架构架,描述了该构架的主要结构特点,分析了其工艺难点,提出了合理的焊接工艺措施。     

完善动车组运用检修体系，确保动车组安全稳定运行

CRH2型动车组的原型车是日本东日本铁路客运公司的E2—1000型动车组，2001年开始制造，最高运行速度275km／h，编组10辆，8动2拖。CRH2型动车组技术引进后，根据国情需要进行了改造，主要是将编组8辆改为4动4拖；使用国产的DSA250型受电弓，同时装设了自动过分相设备；控车使用了ATP系统、LKJ2000运行监控记录装置两套设备；通讯方式采用CIR系统和无线列车调度设备。在车内服务设施、     

AWD四驱时代 从特性认识AWD

很多人被汽车的分类给搞得不知所措，有按车型分的，有按车身尺寸分的，还有按照驱动形式来分的。在这里，我们着重对汽车驱动形式中的四轮驱动技术进行一些介绍，尤其是AWD全时四驱。     

特困大学生心理障碍分析

本文论述了特困大学生中常见的心理障碍及其危害，分析了造成特困大学生心理障碍的主要原因，提出了引导特困大学生克服心理障碍的主要对策。