Stewart型并联平台跃度映射

以刚体速度的旋量描述为基础,推导刚体跃度的旋量表达式.在此基础上利用旋量的李括弧运算,得到6自由度UPS串联机构的跃度映射.将串联机构的跃度映射推广到并联机构,建立Stewart型并联平台的跃度映射模型,得到表征跃度映射的三阶影响系数矩阵.该矩阵为四维矩阵,只与机构的位型有关,根据机构类型、运动学尺寸及广义坐标,即可直接得到该矩阵.以XYZ欧拉角为例,推导出上平台的姿态三阶导数与其角跃度的变换公式.最后通过虚拟样机仿真和理论计算的对比验证了跃度映射模型的正确性.     

浅谈减振器外特性

减振器的外特性是评价减振器性能的重要指标之一。分析了示功特性和速度特性的设计要求，阐述了外特性各种阻尼力的影响因素和外特性曲线畸变的产生原因，同时提出了解决的方法。     

PC连续刚构梁桥合拢时机的分析

混凝土后期的徐变对超静定结构的连续刚构桥产生附加内力,对结构产生不利影响。基于有限元原理,以太澳高速公路永胜特大桥为工程背景,计算分析了不同施工合拢时机对连续刚构桥运营期腹板主拉应力、跨中下挠度的影响,探讨了该类型桥梁合拢时机选择的一般规律,提出该类型桥梁可行的施工合拢时机与理论依据,对同类型桥梁的分析、施工有一定参考价值。     

THE STUDY OF MICROSATELLITES ALTERATION IN DIAGNOSES OF BLADDER CANCER

Objective To investigate the possibility of microsatellite alteration （MA） in diagnosis of bladder cancer of Chinese people, and find the better panel which will be used in clinic. Methods A total of 6 and 10 microsatellite markers were chosen, PCR-SSLP silver staining assay was performed in 31 and 32 bladder cancers tissue, exfoliate cells in urine and 10, 15 non-bladder cancers exfoliate cells in urine, respectively. Results MA （＋） was found in 28 out of 31, 30 out of 32 bladder cancers, and the sensitivity was 90. 3%, 93.7% respectively. The MA of urine sediment of 25 non-bladder cancers was negative, and the specificity was 100%. The cytology was carried out among 19 out of 31, 20 out of 32 bladder cancers at the same time, 2 cases （10.3%） and 3 cases （15%） were found cancer positive, and the sensitivity is significantly lower than that by the analysis of MA in exfoliated cells. Conclusion MA was not associated with grade and stage of the bladder cancer. MA assay is a sensitive and effective method for the early detection of bladder cancer and post-operation surveillance.     

大气激光通信中几种调制方式的性能比较

为选择适合大气激光通信的信道调制方式,对湍流信道下的大气激光通信系统中的OOK,PPM,DPPM和DPIM调制方式的包误码率(PER)和带宽性能进行了仿真分析.结果表明,DPIM的PER性能最好,PPM和DPPM次之,OOK最差.当调制参数较小时,PPM的带宽性能较好;当调制参数较大时,DPPM和DPIM的带宽性能较好.除OOK之外的3种调制方式需要的功率都较小.     

一种改良式质粒DNA提取方法的建立与应用

目的建立一种稳定、可靠的质粒DNA提取改良方法,探讨其在分子生物学实验研究中的应用价值。方法将含有目的质粒的菌株扩增后,运用改良后的碱裂解法进行质粒DNA小量提取,微量核酸仪测定提取质粒DNA的产量及纯度;采用琼脂糖凝胶电泳、内切酶酶切及质粒转染真核细胞鉴定提取质粒DNA的质量及转染效率。结果改良式质粒提取方法获得质粒DNA产量为3.2 mg/L菌液,A260/280=1.91;内切酶酶切完全;质粒DNA以超螺旋结构为主;真核细胞转染效率为20%左右。结论改良式质粒DNA抽提方法操作简单、实用,获得质粒DNA产量多、质量高,能达到分子生物学常规实验的要求。     

高涑公路特长隧道Ⅱ类围膏盐浅埋段施工技术

结合重庆市万州-开县高速公路G1合同段铁峰山2#隧道的施工实践，对该隧道Ⅱ类软质岩层膏盐浅埋段的施工工艺和施工方法作一介绍，并提出若干建议和看法，可为同类施工提供借鉴。     

REAL-TIME DETECTION OF SURVIVIN mRNA EXPRESSION IN CERVICAL CANCER CELL LINES USING MOLECULAR BEACON IMAGING

Objective To detect the expression of survivin mRNA in cervical cancer cell lines using molecular beacon imaging technology. Methods Human cervical cancer cells （HeLa and SiHa） and human fetal lung fibroblast HFL-I were cultured in vitro. After adding 100 nmol/L survivin mRNA molecular beacon, the fluorescent signals were observed under fluorescent microscope. The expressions of survivin in cervical cancer cells and HFL-I cell were examined by immunocytochemical streptravidin-biothin peroxidase （SP） assay at the same time. Results Two kinds of survivin mRNA molecular beacon, with different color fluorescence, had strong fluorescent signal in cervical cancer cell lines, and the signal in SiHa cell line was stronger, but these signals were not found in HFL-I ; Immunocytochemical staining of positive survivin was located in the cytoplasm of cervical cancer cell lines HeLa and SiHa, whereas, no expression of survivin was detected in HFL-I cell line. Conclusion The technology of molecular beacon imaging can be used to detect the expression of survivin mRNA in viable cells successfully, and may provide a new approach to the diagnosis of early stage cervical cancer and the following-up in the clinic.     

山地城市干线交通控制子区划分方法研究

山地城市干线承载了道路交通较大比例的出行需求,单一固定的管控手段难以适应当前复杂的交通环境,需要协调控制才能满足交通管理需求。交通控制子区划分作为协调控制的首要任务,影响着交通管控效果的优劣。当前交通子区划分研究重点已从静态交通控制子区划分转变为动态交通控制子区划分,主要集中在关联度指标计算、子区划分算法等方面。针对目前子区划分方法与实际应用关联度不足,且不太适用于山地城市干线的局限,基于交通管控应用分析了山地城市干线多维不均衡性,从数据库构建、关联度建模、子区划分算法等方面提出了子区划分算法思路,构建了基于距离、流量、交通流结构的多因素山地城市干线交通控制子区划分方法。