水泥稳定风积砂作为路面基层材料的试验研究

采用均匀设计的方法来优化试验,以水泥、碎石、风积砂为原材料进行试验研究,得到水泥稳定风积砂的最佳含水量、最大干密度、无侧限抗压强度、回弹模量等指标,并通过SPSS软件进行回归分析,建立回归方程.研究结果表明水泥用量对无侧限抗压强度的影响最大,其次是碎石的用量,风积砂的用量对强度的影响较小.通过铺筑试验路的试验和观测,结果表明,水泥稳定风积砂基层状况良好,且弯沉值满足有关的要求.     

CLONING SEGMENT SPIKE PROTEIN GENE OF SARS-COV AND ITSEXPRESSION IN ESCHERICHIA COLI

Objective Expressing and purifying the seglnent of SARS-CoV spike protein in E. Coli Methods The target gene was obtained by RT-PCR. The PCR product was cloned into pEGM- T Easy Vector, sequencing and double restriction digestion (BamH Ⅰ , Pst Ⅰ) were performed. The target gene was subcloned into PQE30 expression vector. The gene was expressed in the E. coli strain M15 cells induced by IPTG. The protein was purified with a nickel HiTrap chelating metal affinity column. Results The recombinant expression plasmid was successfully constructed and the protein was well expressed in E. coli strain M15 cells. The ideal pure protein was obtained by purification. Western blotting analysis suggested the protein could act with the convalescent sera of lab confirmed SARS patients. Conclusion The segment of SARS-CoV spike protein was well expressed and purified, and can be applied in diagnosis and immunological research of SARS.     

桥梁现场常见病害及检查

建于上世纪60、70年代的公路桥梁,现在大多带病服务于现代交通运输中,因此,对这些桥梁进行病害检查就显得十分重要。桥面系、上部构造、支座、墩台及基础是病害检查的重要部位。文中指出了这些部位的常见病害,并分析了产生这些病害的原因,为桥梁的养护决策提供依据。     

葡萄糖、胰岛素和尿酸对猪近端肾小管上皮细胞株血红素氧合酶-1的影响

目的观察不同浓度葡萄糖、胰岛素和尿酸的培养条件下猪近端肾小管上皮细胞株(LLC-PK1)血红素氧合酶(HO-1)活性的改变,以探讨代谢相关因素在细胞水平对肾小管上皮细胞的影响。方法以LLC-PK1为研究对象,观察葡萄糖浓度为5、10、22、33 mmol/L,胰岛素浓度为0、10-9、10-8、10-7mol/L,尿酸浓度为0、0.1、0.2、0.4 mmol/L培养48 h条件下,LLC-PK1细胞HO-1活性的改变。结果0.1 mmol/L0、.2 mmol/L和0.4 mmol/L尿酸刺激48 h后,LLC-PK1的HO-1活性增加,并呈剂量依赖关系,而上述浓度的葡萄糖和胰岛素刺激48 h对LLC-PK1的HO-1活性无影响。结论一定浓度的尿酸可使LLC-PK1的HO-1活性升高,提示尿酸在细胞水平对肾小管上皮细胞的氧化应激过程产生影响。     

单壁钢围堰在大潮差桥梁桩基加固中的应用

桥梁水中桩基在江、河、海水的日益冲刷下出现混凝土剥落、钢筋外露锈蚀,导致桩基缩径,极大地威胁着桥梁结构的安全性,这就需要对桩基进行维修加固。结合《大潮差桥梁桩基钢围堰施工技术研究》(S20130013)和温州市东瓯大桥桩基加固工程实例,介绍单壁钢围堰技术在处理水下桩基础破损露筋等质量问题中的应用,阐述了钢围堰加固桩基施工方案的实施和施工中应注意的几个关键技术。     

乐平市接渡大桥总体设计

乐平市接渡大桥是一座公路兼城市桥梁，江西省内目前第一座公路钢桁架桥，桥位巧妙的利用平面线形避开现状老桥，采用老桥保障施工期间的社会正常通行需求，做到了“零拆迁”产生了较好的经济和社会效应。主桥采用跨径(60+60)m的下承式连续钢桁架桥，桥面宽19.6m，新桥与现状老桥桥面净距7.9m，新桥建成后对老桥采用静力切割法拆除，确保了新建桥梁的安全。如何利用现状老桥保障施工期间正常通行以节约工程造价、满足Ⅴ-（3）通航净空要求、桥头两岸建筑“零拆迁”是项目的重难点，以该桥为工程背景，介绍桥梁总体设计构思，以期对类似工程有借鉴作用。     

双浮体波浪能装置的振荡和能量转换特性研究（英文）

In this study, we investigated the hydrodynamic and energy conversion performance of a double-float wave energy converter(WEC) based on the linear theory of water waves. The generator power take-off(PTO) system is modeled as a combination of a linear viscous damping and a linear spring. Using the frequency domain method, the optimal damping coefficient of the generator PTO system is derived to achieve the optimal conversion efficiency(capture width ratio).Based on the potential flow theory and the higher-order boundary element method(HOBEM), we constructed a threedimensional model of double-float WEC to study its hydrodynamic performance and response in the time domain. Only the heave motion of the two-body system is considered and a virtual function is introduced to decouple the motions of the floats. The energy conversion character of the double-float WEC is also evaluated. The investigation is carried out over a wide range of incident wave frequency. By analyzing the effects of the incident wave frequency, we derive the PTO's damping coefficient for the double-float WEC's capture width ratio and the relationships between the capture width ratio and the natural frequencies of the lower and upper floats. In addition, it is capable to modify the natural frequencies of the two floats by changing the stiffness coefficients of the PTO and mooring systems. We found that the natural frequencies of the device can directly influence the peak frequency of the capture width, which may provide an important reference for the design of WECs.     

工业副产品木质素的岩土工程应用与发展

生物能源副产品木质素是一种不污染环境,且成本低廉的新型高效土体固化剂,适用于地基土加固和增强土体抗侵蚀性,目前已在欧美发达国家广泛开展研究并得到实际应用,且有很多成功的工程实例。通过文献回顾,总结了国内外对木质素加固土体性能的评价方法和典型室内试验结果。同时论述了木质素加固土体的抗侵蚀特性,归纳了相关木质素加固土体抗侵蚀特性的室内试验方法。针对目前国内已有研究现状,探讨了生物能源副产品木质素在加固路基土中存在的问题,展望了其在岩土工程中的应用前景。     

城市轨道交通移动闭塞列控系统列车追踪间隔研究

目前,基于通信的移动闭塞列控系统作为轨道交通列控系统的主要发展方向,在一定程度上缩短了列车之间的追踪间隔。追踪间隔的计算是列车生成移动授权的前提。列控系统中移动授权的发布由区域控制器来完成。列控系统中由于追踪模式的不同,列车追踪间隔也会有差异,从而影响移动授权生成,影响行车效率。分析了列车移动授权生成原理,研究了列车区间追踪场景下绝对追踪模式和相对追踪模式下的列车追踪间隔,并进行了仿真。仿真分析结果表明:相对追踪模式下列车生成的移动授权更大,可以进一步缩小列车追踪间隔;绝对追踪模式存在最优追踪速度。     

论大型企业档案信息服务

近年来,我国各类大型企业集团加快了档案信息化的步伐,不断提高档案信息服务水平,为企业发展提供保障。然而在档案信息化建设中仍存在不少问题,档案信息化工作有待改进。本文分析了当前企业档案信息化建设和信息服务的现状以及存在的问题,提出了改进对策。