Construction of lentivirus vectors carrying alphastatin gene and its secretion expression in human umbilical vein endothelia cells

Objective To construct lentivirus vectors carrying alphastatin gene, test its secretion expression in human umbilical vein endothelia cells (HUVECs) and observe its effects on growth, migration and tube formation of HUVECs. Methods We constructed recombinant lentivirus vectors of NT4-alphastatin fusion gene containing neurotrophin-4 signal peptide, pro-region sequences and alphastatin, then transfected the recombinant lentivirus vectors into HUVECs to obtain secretory protein alphastatin and test its anti-angiogenic activities in vitro. Results Our data showed that recombinant self-inactivating lentivirus vectors of NT4-alphastatin were successfully constructed, and stable NT4-alphastatin transduced HUVECs were capable of sustainably secreting alphastatin which significantly suppressed HUVECs migration and differentiation but not VEGF-induced proliferation. Conclusion This report represents the first time on the use of lentivirus-based vectors to deliver alphastatin, the endogenous angiogenesis inhibitor, and reveals the potential utility of anti-angiogenic gene therapy with lentivirus vectors for treating cancer.     

基于节能和面向旅客服务的列车编组方案研究

从城市客流出行特征分析入手,探讨了城市轨道交通在不同运营时期列车编组方案的可能性和存在的问题.提出了近期采用最大编组一半的小编组列车、远期采用可解列和连挂的灵活编组方案,以提供满足不同时期以及每日不同时段的运能要求和高水平运营服务.通过对列车不同满载率情况下列车牵引耗能的初步定量分析,以及对其他相关技术问题进行了分析后,认为在平峰时段采用小编组运营将有效减小运营支出和显著提高服务水平.     

地铁施工中的轨道运输组织

地铁施工阶段的轨道运输是地铁建设中的重要一环.施工阶段的运输组织存在较多的困难因素.在总结历次地铁建设经验的基础上,特别是通过北京地铁5号线的施工,对地铁施工阶段轨道运输的重要性、概况、困难及克服这些困难的方法进行了分析和总结.     

路基石方控制爆破施工方法及施工控制

该文对路基石方控制爆破施工实践进行了分析总结,探讨了路基石方控制爆破施工中应注意做好的重点工作,可为同类工程施工控制提供借鉴。     

INFLUENCE OF ETHANOL ON TOXICOKINETICS OF ACONITINE

ＩＮＦＬＵＥＮＣＥＯＦＥＴＨＡＮＯＬＯＮＴＯＸＩＣＯＫＩＮＥＴＩＣＳＯＦＡＣＯＮＩＴＩＮＥＸｕＷｅｉ；ＨｕＢｉｎｇｗｅｉ；ＣｈｅＸｉａｎｇｍｉｎｇ（ＤｅｐａｒｔｍｅｎｔｏｆＴｏｘｉｃｏｌｏｇｙ，ＦａｃｕｌｔｙｏｆＦｏｒｅｎｓｉｃＭｅｄｉｃｉｎｅ，Ｘｉ...     

从环保角度看城市轨道交通的轨道结构

从环境保护的角度对城市轨道交通的轨道结构标准提出具体的要求，并对高架线和地铁线采用无碴轨道结构和新型轨下基础以达到减振降噪的目的提出建议。     

On the application of the Haskind-Hanaoka relations to hydroelasticity problems

The Haskind-Hanaoka relations for obtaining the wave-exciting forces, given the incoming and radiation potentials in floating rigid-body dynamics, are extended to deformable-body dynamics in the presence of a free surface and within the assumption of linear potentials theory. These relations are applied to the mode-superposition and the direct methods of solution of hydroelasticity problems. In other words, it is shown that regardless of the structural analysis method used in a hydroelasticity problem, the Haskind-Hanaoka relations can be used to calculate the wave-exciting forces without solving for the diffraction potential explicitly. These relations can also be used in the composite singularity distribution method to increase further the computational efficiency.     

疲劳荷载作用下PPC箱形约束梁斜截面抗裂性试验研究

本文根据四片PPC箱形约束梁的斜截面疲劳抗裂性试验结果,分析了试验梁在疲劳荷载作用下的受力特点,认为PPC箱形约束梁的疲劳抗裂性较相应的简支梁更为不利,并探讨了PPC箱形约束梁斜截面疲劳抗裂性的计算原理和方法。     

15MnMoVNRe、StE690、HQ80高强钢冷裂敏感性与焊接工艺的选择

对15MnMoVNRe等低合金高强钢的冷裂敏感性进行了试验研究,并制定了既能避免冷裂纹产生,又可防止热影响区脆化的焊接工艺。     

术前放疗对直肠腺癌及正常粘膜上皮细胞超微结构的影响

通过对１１例直肠腺癌及周围粘膜上皮细胞超微结构的电镜观察，对比研究了术前放疗引起的直肠腺癌超微结构的改变。提示放疗后病人出现的不同程度的症状减轻与瘤细胞坏死，瘤体缩小有关；粘液稀便减少与放疗所致的粘膜损伤，特别是杯状细胞的破坏直接相关。为术前放疗理论提供了病理学基础。