Construction of lentivirus vectors carrying alphastatin gene and its secretion expression in human umbilical vein endothelia cells

Objective To construct lentivirus vectors carrying alphastatin gene, test its secretion expression in human umbilical vein endothelia cells (HUVECs) and observe its effects on growth, migration and tube formation of HUVECs. Methods We constructed recombinant lentivirus vectors of NT4-alphastatin fusion gene containing neurotrophin-4 signal peptide, pro-region sequences and alphastatin, then transfected the recombinant lentivirus vectors into HUVECs to obtain secretory protein alphastatin and test its anti-angiogenic activities in vitro. Results Our data showed that recombinant self-inactivating lentivirus vectors of NT4-alphastatin were successfully constructed, and stable NT4-alphastatin transduced HUVECs were capable of sustainably secreting alphastatin which significantly suppressed HUVECs migration and differentiation but not VEGF-induced proliferation. Conclusion This report represents the first time on the use of lentivirus-based vectors to deliver alphastatin, the endogenous angiogenesis inhibitor, and reveals the potential utility of anti-angiogenic gene therapy with lentivirus vectors for treating cancer.     

基于参考模型的半主动悬架滑模控制

在分析电流变阻尼器工作原理与结构的基础上,基于参考模型设计了1/4车辆悬架系统的滑模控制器.研究了系统在随机路面激励条件下车身加速度、悬架动行程和轮胎动位移等性能指标的控制效果.运用Simu-link在不同的车速和车身质量的情况下进行了仿真分析,结果表明:控制后悬架各性能指标均得到明显改善,滑模控制器性能稳定,对系统参数的改变具有很好的鲁棒性.     

基于节能和面向旅客服务的列车编组方案研究

从城市客流出行特征分析入手,探讨了城市轨道交通在不同运营时期列车编组方案的可能性和存在的问题.提出了近期采用最大编组一半的小编组列车、远期采用可解列和连挂的灵活编组方案,以提供满足不同时期以及每日不同时段的运能要求和高水平运营服务.通过对列车不同满载率情况下列车牵引耗能的初步定量分析,以及对其他相关技术问题进行了分析后,认为在平峰时段采用小编组运营将有效减小运营支出和显著提高服务水平.     

三门口跨海大桥陡坡裸岩区域钻孔桩基础施工技术

针对浙江省象山县三门口跨海大桥岸边陡坡裸岩处桥墩钻孔桩施工中的难点,介绍了施工中通过采用水下剪刀撑、三角网格连接、水平承力系统拉结、灌注混凝土配重等技术措施,加强了钻孔平台的整体性和稳定性,成功完成了钻孔桩施工及承台钢吊箱围堰的施工作业。     

防空导弹武器系统目标分配建模研究

目标分配是防空导弹作战指挥控制中的关键环节,分配的优劣直接关系到防空导弹武器系统的作战效果及整体效能的发挥。文章根据防空导弹作战的实际特点,提出目标分配模型建立的假设条件,建立了基于马尔可夫决策过程的最优化动态WTA方法。最后用实例验证模型的准确性,所得结果符合作战实际,为作战指挥员科学、合理分配目标提供数据支持。     

INFLUENCE OF ETHANOL ON TOXICOKINETICS OF ACONITINE

ＩＮＦＬＵＥＮＣＥＯＦＥＴＨＡＮＯＬＯＮＴＯＸＩＣＯＫＩＮＥＴＩＣＳＯＦＡＣＯＮＩＴＩＮＥＸｕＷｅｉ；ＨｕＢｉｎｇｗｅｉ；ＣｈｅＸｉａｎｇｍｉｎｇ（ＤｅｐａｒｔｍｅｎｔｏｆＴｏｘｉｃｏｌｏｇｙ，ＦａｃｕｌｔｙｏｆＦｏｒｅｎｓｉｃＭｅｄｉｃｉｎｅ，Ｘｉ...     

从环保角度看城市轨道交通的轨道结构

从环境保护的角度对城市轨道交通的轨道结构标准提出具体的要求，并对高架线和地铁线采用无碴轨道结构和新型轨下基础以达到减振降噪的目的提出建议。     

On the application of the Haskind-Hanaoka relations to hydroelasticity problems

The Haskind-Hanaoka relations for obtaining the wave-exciting forces, given the incoming and radiation potentials in floating rigid-body dynamics, are extended to deformable-body dynamics in the presence of a free surface and within the assumption of linear potentials theory. These relations are applied to the mode-superposition and the direct methods of solution of hydroelasticity problems. In other words, it is shown that regardless of the structural analysis method used in a hydroelasticity problem, the Haskind-Hanaoka relations can be used to calculate the wave-exciting forces without solving for the diffraction potential explicitly. These relations can also be used in the composite singularity distribution method to increase further the computational efficiency.     

疲劳荷载作用下PPC箱形约束梁斜截面抗裂性试验研究

本文根据四片PPC箱形约束梁的斜截面疲劳抗裂性试验结果,分析了试验梁在疲劳荷载作用下的受力特点,认为PPC箱形约束梁的疲劳抗裂性较相应的简支梁更为不利,并探讨了PPC箱形约束梁斜截面疲劳抗裂性的计算原理和方法。     

15MnMoVNRe、StE690、HQ80高强钢冷裂敏感性与焊接工艺的选择

对15MnMoVNRe等低合金高强钢的冷裂敏感性进行了试验研究,并制定了既能避免冷裂纹产生,又可防止热影响区脆化的焊接工艺。