Study of the mechanism on the apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase

Objective To investigate the mechanisms of apoptosis induced in Human leukemia cell line K562 by the combination of indole-3-acetic acid and horseradish peroxidase. Methods Human leukemia cell line K562 were exposed to indole-3-acetic acid （IAA） at 20, 40, 60, 80 or 100 mol/L and horseradish peroxidase（HRP） at 1.2 g/mL for varying times. MTT assay was applied to detect the cell proliferation. Flow cytometry was performed to detect the arrest of cell cycle. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling （TUNEL） assay was used to measure apoptosis. 2, 7-dichlorofluorescin diacetate （DCFH-DA） uptake was measured to determine free radical by confocal microscope. Content of malondiadehyde （MDA） and activity of superoxide dismutase （SOD） were measured by biochemical methods. Results IAA/HRP initiated growth inhibition of K562 cells in a dose- and time-dependent manner. Flow cytometry revealed that cell cycle arrested at G1/G0 after 24 hours treatment. After 72 hours treatment, apoptotic rate of 100 mol/L IAA group increased to 43.9 %, which was 5 times that of control（P〈0.01）. Content of MDA and activity of SOD increased respectively in treatments compared with control. Meanwhile, IAA/HRP stimulated the formation of free radical, which was increased by IAA concentration-dependently. Conclusion The combination of IAAand HRP can inhibit the growth of Human leukemia cell line K562 in vitro by inducing apoptosis which is associated with the increase of free radical. The combination of IAA and HRP might be a promising chemopreventive and chemotherapeutic agent against human leukemia.     

高速公路路面车辙病害的微表处修补技术

本文主要从车辙的产生、车辙类型等方面进行分析、分类,针对高速公路不同程度的车辙采取不同的修补方法,从而确保高速公路的路况满足车辆行驶要求,同时,通过对车辙修补,达到延长高速公路路面的使用寿命、降低工程保养成本的目的。     

生物接触氧化法处理洗浴废水的填料选择研究

生物接触氧化法处理洗浴废水的填料选择研究以软性纤维和软性泡沫作为备选填料,采用活性污泥挂膜法,浅析了膜培养过程中的影响因素.最后通过最佳水力停留时间这一废水处理指标对备选填料进行比较,最终选择软性泡沫作为动态实验填料.     

基于细胞自动机方法的车行运动模型及信号灯控制策略研究

采用细胞自动机方法研究了在单向道路上行驶的2辆不同性能(加速度能力、最大速度等)车辆的运动状态和相互关系,以及性能参数对行驶状态的影响.讨论了在设置路口和信号灯的情况下,不同信号灯控制策略(信号灯的时间周期及绿信比)对车辆运动状态的影响.细胞自动机方法通过JAVA编程进行数值模拟,得到车辆行驶时间-位置之间的关系,结果与预期相同.该模型退化为匀速运动状态情形时,所得结果与近期文献的结果相一致,也证明了文中所用方法的可靠性和合理性.     

HZQ900型架桥机高位驮运转场安全性计算分析

以武广铁路客运专线HZQ900型架桥机高位驮运转场为例,采用有限元分析软件分析了转场过程中支撑设备的强度、刚度和桥机自身的横向、纵向抗倾覆稳定性,结果表明所述方案能够实现桥机高位驮运转场安全、经济和高效的目标,对类似工程具有一定的指导和借鉴作用。     

油气弹簧阀片均布载荷变形研究与数学建模

根据节流阀片物理模型以及薄板力学的相关理论,推导阀片受均布载荷作用时挠曲变形量的解析式,并整理得出便于理论分析和工程应用的表达方式.根据自主研发油气弹簧的结构特点,运用实际气体状态方程,建立单气室油气弹簧的数学模型.仿真分析系统弹性力与不同叠加阀片数量下总输出力随位移的变化关系,与试验数据相比较,验证了数学模型的正确性以及节流阀片变形解析式的实用价值,为阻尼阀的精确设计提供了依据.     

个体因素对选择自行车通勤的影响研究

考虑出行者在个体因素方面的差异及其对选择自行车通勤的作用,利用Odds Ratio统计定性地分析各因素的影响,运用因子分析探索因素间的相关性和内部联系.基于因子分析结果,建立含隐含变量的二项Logit（BL）模型.估计结果表明：出行距离、感知收益、小汽车拥有与是否自行车通勤显著相关.同时利用普通BL模型作为参考得到补充和比较结果：尽管社会经济地位是显著的,家庭收入的影响却不大;出行者更在意快速性和便利性收益,而非安全性和舒适性收益.     

Effects of Panax notoginseng saponins on hydrogen peroxide-induced apoptosis in cultured rabbit bone marrow stromal cells

Objective To investigate the effects of Panax notoginseng saponins (PNS) on hydrogen peroxide (H2O2)-induced apoptosis in cultured rabbit bone marrow stromal cells (BMSCs). Methods BMSCs from 3-month-old New Zealand rabbits were isolated and cultured by the density gradient centrifugation combined with adherent method. The cultured BMSCs were divided into three groups: normal control, H2O2 treatment (100μmol/L), and PNS pretreatment (0.1g/L). Intracellular reactive oxygen species (ROS) levels as the index of oxidative stress were measured by using 2'7'-dichlorodihydrofluorescein diacetate. Flow cytometry was used to observe the apoptosis of BMSCs by staining with annexinV-FITC/PI. The protein expression of Bax in BMSCs was analyzed by Western blotting. Activity of caspase-3 enzyme was measured by spectrofluorometry. Results Pretreatment with PNS significantly decreased intracellular ROS level induced by H2O2 (P<0.01). PNS markedly attenuated H2O2-induced apoptosis rate from 38.68% to 19.24%(P<0.01). PNS reversed H2O2-induced augmentation of Bax expression. Furthermore, PNS markedly reduced the altered in activity of caspase-3 enzyme induced by H2O2(P<0.01). Conclusion PNS has a protective effect on hydrogen peroxide-induced apoptosis in cultured rabbit BMSCs by scavenging ROS and decreasing Bax expression and caspase-3 activity.     

制约部队水路快速输送的主要因素及对策

部队水路快速输送能力由诸多相关因素决定,综合实际情况分析,制约部队水路快速输送的主要原因有:港口类型失衡、港口装卸能力偏弱、民用船舶征集难和船舶改装难等。为提高部队水路快速输送能力,提出加大备用码头建设、预先制定装载方案、加快法律法规的完善、推进军事运输机制的改革、加强民船改装研究力度等建议。对于提高港口保障能力和船舶保障能力,以及提高部队水路快速输送能力具有重要意义。     

基于NMEA2000协议的船舶集成网络系统研究

随着船舶自动化程度中不断提高和计算机网络技术的快速发展,船舶自动化系统正在向船舶信息集成化方向发展.文中引入NMEA2000协议标准,分析了协议的网络特征和分层结构,提出船舶集成网络系统的三层结构,即设备网络、以太网及无线通信网,阐述了网络系统的拓扑结构、功能及设备组成.基于NMEA2000协议的船舶集成网络系统是船舶的信息基础设施,将为各种船载电子设备及应用系统提供全面的信息交换环境.