EXPRESSING HUMAN MATURED BRAIN-DERIVED NEUROTROPHIC FACTOR GENE IN E. Coli AND DETERMINING ITS BIOACTIVITY

Brain-derived neurotrophic factor (BDNF)is amember of the family of neurotrophins['J. The hu-man BDNF gene is localized on chromosome of1lp13. The nucleotide sequence that is 744bpIength including signal sequence and prosequenceshows an open reading frame to encode a precursorof 247 amino acids. The matured BDNF consists ofll9 amino acids (relative molecular mass of 14Kd,PI~l0)['.'j. The activity of BDNF in the nervoussystem is widespread. BDNF could support the sur-vival of cranial …     

长缨剑胆舒——中国船级社走过45年

无论世界怎么变化,一种传统的交流手段始终影响着人们的生活--那就是船舶和航运. 占地球表面积约71%的海洋将陆地分割,这就决定了各国之间的物质交流必须依靠海上交通.统计表明,国际贸易的90%的份额由海上船舶运输.而承担海上风险管理的公益性机构--船级社,则是船舶在浩瀚海洋安全航行的有力保障.     

用P-Y曲线法计算板桩结构

根据P-Y曲线法原理分别对沉入砂性土地基及粘性土地基的板桩码头进行了例题计算,并且与传统的m法进行了比较。     

船舶模拟器驾驶台仿真系统研究

本文以用于三峡船闸运行过程仿真的船舶模拟器为背景,介绍了船舶模拟驾驶台的结构及设计方案,以内河航运船舶为对象构成了驾驶台半实物仿真系统,建立了主机、舵机数学模型,对模拟驾驶台的结构,仿真方法作了详尽的研究。     

基于遗传优化的水面无人船艇神经网络智能控制

利用GA智能优化算法和RBF神经网络逼近算法设计了一种USV运动滑模理想跟踪控制方法.首先利用改进的遗传算法对RBF网络参数进行在线寻优以进而提高其逼近性能.其次,将学习速度较快的局部RBF神经网络对滑模控制设计中存在的船舶运动系统函数不确定项进行逼近,使得由于滑模面的不间断切换引起的控制输入抖振问题得到有效地解决.对比实验说明了在同等条件下,上述智能控制系统稳定时间更快,超调量更小,以及输入舵角更平滑.     

Geometric calibration algorithm of polarization camera using planar patterns

Polarization pattern provides additional information besides spectral signatures. It can be used in many applications, such as navigation, defect detection and object identification. A novel polarization camera composed of four synchronized cameras is proposed, and it can realize real-time polarization measurement. This study particularly concentrates on the geometric calibration of the system. The projection model is analyzed and the multi-camera calibration algorithm is proposed. Firstly, each camera is calibrated separately using planar patterns, and then the geometric calibration algorithms are performed. Due to the geometrical constraint, a global optimization method results in smaller estimation uncertainties. A mean rotation error of 0.025° and a mean translation error of 0.26mm are achieved after geometric calibration. The images are rectified to establish a correspondence among cameras and are combined to acquire the polarization measurement. The polarization pattern of the skylight is measured by the system and the results are consistent with the previous studies.     

Uncertainty analysis of value for money assessment for public-private partnership projects

In recent years, private sectors are encouraged to take an active part in franchising of urban infrastructure investments and operations, which promotes the rapid development of public-private partnership (PPP) in infrastructure and public service supply. Value for money (VFM) assessment has been officially proposed to provide a reference for selection of projects planning to adopt PPP. Based on the bottlenecks of VFM application in China and the uncertainties for urban infrastructure PPP projects, a discounted cash flow (DCF) model is established for VFM of infrastructure PPP projects. Then, a Monte Carlo simulation model is established on the basis of uncertainty factors for VFM. Through the analysis of Huai’an trams PPP project, coping strategies of uncertainties for VFM are put forward. Findings of the research may propel the establishment of a complete VFM evaluation system for PPP projects. Key instructional functions of VFM during the process of decision-making can be brought into full play and PPP may develop orderly.     

信号控制下人行横道宽度优化模型

为了提高行人过街安全,本文分析了信号控制下行人过街的时空分布特性,研究行人过街空间区域最大宽度与时间和人行横道纵向位置映射关系;通过对行人流量和分担率两因素的分析,对行人过街空间区域特性进行数学描述,根据实测数据并利用轨迹提取技术获取行人时空数据,建立了满足过街时间约束下的人行横道宽度优化模型;最后利用长春市4处人行横道的行人过街时空信息,以群体过街时间为验证指标对模型进行了验证,结果显示,利用本文建立的人行横道宽度优化模型所确定的行人过街时间在3处地点分别减少5.60%、3.29%和2.58%.本文建立的基于时空安全的人行横道宽度优化方法可为人行横道宽度的设计提供理论参考和技术支持.     

人工湖旁修建浅埋富水隧道暗挖技术

通过一个在湖旁用暗挖法修筑的浅埋富水隧道的实例,阐述在湖旁修筑浅埋富水地下隧道的暗挖技术。     

多年冻土路基水分迁移热力学性能分析

从水分场与温度场相互作用的角度,分别建立温度场单场控制方程和水热耦合效应的控制方程,应用有限元方法进行计算。通过对单因素温度场的计算结果与水热耦合作用下的温度场计算结果比较,分析水分迁移作用对路基温度场的影响。