水飞蓟宾对人膀胱癌细胞株5637增殖的抑制作用及其机制

目的研究水飞蓟宾(Silibinin,SB)在体外对人膀胱癌细胞株5637增殖的抑制作用及其相关机制。方法采用MTT法观察不同浓度SB对人膀胱癌细胞株5637增殖的抑制作用;倒置显微镜下观察细胞形态变化;RT-PCR检测凋亡抑制因子Survivin的mRNA表达变化。结果MTT检测发现:随着SB浓度增加和作用时间延长,对人膀胱癌细胞株5637增殖的抑制作用增强,且各组间比较均有显著性差异(P<0.01),并呈时间-剂量依赖关系;倒置显微镜发现应用SB干预后,5637细胞体积缩小,变形,胞浆浓缩,核固缩深染;人膀胱癌细胞株5637中Survivin的mRNA表达随着SB浓度增加和时间延长相应下降。结论SB可抑制人膀胱癌细胞株5637的增殖;并下调凋亡抑制因子Survivin的mRNA表达。     

首席工人制打造公交企业蓝领精英

公交企业如何打造自己的蓝领精英,培养企业自己的技术工人,给技术工人创造良好的发展环境,已成为重要的管理课题.我公司自2004年实行首席工人制以来,培育出了一批优秀的蓝领精英,对缓解高超技术工人缺乏,加快企业技术进步,提高企业效益都起到了积极作用.     

公路隧道新设计规范中几个问题的探讨

设计规范作为行业强制性设计要求为依据,应是严谨、严肃的,但它同时又有一个不断发展和完善的过程.通过对新《公路隧道设计规范》(JTG D70—2004)使用中发现的与其它相关标准、规范的协调性、隧道建筑限界与内轮廓、钢纤维喷射砼等几个问题的探讨,起到抛砖引玉的作用,供同行研讨、参考.     

高精度GPS数据处理方法在公路测量中的应用

针对目前高等级公路测量对点位精度要求的提高,提出了对不同载波的GPS相位观测量进行线性组合后,再利用GAMIT软件数据进行处理的方法,试验结果表明,此方法可以在公路控制测量中获得较高GPS定位精度,即在几公里的距离范围,基线精度可达到2 cm,满足公路控制测量的要求.     

基于SWOT分析下的十堰市发展战略

通过对十堰市各种因素的分析,从区域角度论述了城市在发展过程中面临的经济全球化及国家政策等方面的机遇和挑战,从而寻求一个符合十堰特点的城市发展战略。     

关于带误差的Ishikawa迭代过程

在一致凸Banach空间中研究了非扩张映射序列的带误差Ishikawa迭代过程的构造和收敛问题,推广了近期的结果。     

紫杉醇诱导人肺腺癌多药耐药细胞系的建立及DNA polβ、mdr1、mrp1、GST-πl、rp和topo Ⅱ基因的表达

目的体外建立人肺腺癌细胞多药耐药细胞系,观察其生物学特性并探讨其细胞内DNA polβ和多药耐药基因mdr1、mrp1、GST-πl、rp和topo Ⅱ的表达及意义。方法以紫杉醇为诱导药,采用间歇逐步增加剂量法建立人肺腺癌多药耐药细胞系A549/TXL20;MTT法检测A549/TXL20对紫杉醇、顺铂、长春新碱、5-氟尿嘧啶和丝裂霉素的耐药指数(RF);细胞克隆形成法测定A549/TXL20对紫杉醇的敏感性;高效液相色谱测定细胞内紫杉醇的浓度;RT-PCR法测定人肺腺癌多药耐药细胞系A549/TXL20中DNA polβ、mdr1、GST-π、mrp1l、rp和topo Ⅱ的基因表达。结果①A549/TXL20形态不规则,较亲本细胞略小,核/浆比增加,倍增时间没有明显变化;②A549/TXL20对紫杉醇的RF为19.3,对顺铂的RF为67.4,对长春新碱、5-氟尿嘧啶、丝裂霉素和顺铂等抗癌药物有不同程度的耐药性;③A549/TXL20对紫杉醇的敏感性降低,其紫杉醇的含量较亲本细胞下降;④A549/TXL20细胞中DNA polβ、mdr1、GST-π、mrp1l、rp的基因表达量分别较A549细胞中的表达量明显增加,差异具有统计学意义(P<0.05)。结论建立了相对稳定的人肺腺癌多药耐药细胞系A549/TXL20,其耐药机制可能与mdr1、GST-π、mrp1l、rp和DNA polβ基因的高表达有关。     

基于实时Linux计算机联锁系统实时性分析与改进

计算机联锁系统是保障列车在车站范围内安全、高效运行的实时控制系统,它必须具有非常高的安全性、可靠性.这首先应该解决实时性的问题,针对基于Linux下的计算机联锁系统的实时性进行分析,并结合计算机联锁系统的实时性模块给出分析,采用实时Linux系统进行设计与实现,并对改进后的系统与原系统进行测试比较.     

Multi-Band Hybrid Mobile Phone Antenna Based on Electromagnetic Coupling

This research presents a multi-band hybrid mobile phone antenna based on electromagnetic coupling,which can be applied to mobile handheld devices, occupying a small board space of 39.7 mm × 15.6 mm on the system circuit board. By adding resonant and coupled branch instead of multi-feed on the traditional bent antenna,this design provides four wide operating bands of 0.772—0.998 GHz, 1.540—1.600 GHz, 1.680—2.270 GHz and2.300—2.690 GHz with the hybrid feature of planar inverted-F antenna(PIFA), L-shape, U-shape and S-shape structures, which cover nine-band, i.e., GSM850, GSM900, GPS1575, DCS1800, PCS1900, IMT200, LTE2300,LTE2600 and Blue Tooth/Wi-Fi. Ansoft software HFSS is used in this research to make the antenna performance better and the operating principle of the proposed antenna is described in detail. Result of simulation reveals that the maximum gains of these four wide bands are 2.20,-0.99, 4.01 and 4.05 dBi, respectively. Moreover, this research also tests the return loss(S11) of the fabricated antenna with the vector network analyzer and the result is in accordance with the simulation result on the whole. There are four wide resonant frequencies which cover nine-band of wireless wide area network(WWAN), wireless local area network(WLAN) and long term evolution(LTE), when the available bandwidth is better than 6 d Bi return loss.     

Automatic outer surface extraction of femoral head in CT images

Computer-aided hip surgery planning and implant design applications require accurate segmentation of femoral head and proximal acetabulum. An accurate outer surface extraction of femoral head using marching cubes algorithm remains challenging due to deformed shapes and extremely narrow inter-bone regions. In this paper, we present an automatic and fast approach for segmentation of femoral head and proximal acetabulum which leads to accurate and compact representation of femoral head using marching cubes algorithm. At first, valley-emphasized images are constructed from original images so that valleys stand out in high relief. Otsu’s multiple thresholding technique is applied to seperate the images into bone and non-bone classes. Region growing method and threedimensional (3D) morphological operations are performed to fill holes in the bone. In the reclassification process, the bone regions are further segmented, and the boundaries of the bone regions are further refined based on Bayes decision rule. Finally, marching cubes algorithm is applied to reconstruct a 3D model and extract the outer surface of femoral head and proximal acetabulum. Experimental results show that this method is an accurate segmentation technique for femoral head and proximal acetabulum and it can be applied as a tool in medical practice.