自然流产者绒毛组织中印记基因H19的表达及意义

目的探讨印记基因H19在自然流产者绒毛组织中的表达及意义。方法采用逆转录多聚酶链反应(RT-PCR)技术检测45例自然流产患者和30例正常早孕妇女的绒毛组织中H19特殊等位基因表达。结果自然流产患者杂合子绒毛组织21例中19例表达双等位基因,表达率为90.47%(19/21),而正常早孕妇女杂合子绒毛组织13例中H19皆表达单等位基因,无双等位基因表达,表达率为0(0/13),两组比较,双等位基因表达率有统计学意义(P<0.05)。结论H19基因印记丢失可能是造成自然流产的重要原因。     

Distributed Broker-Agent Architecture for Multimedia Communications Traversing NAT／Firewall in NGN

The forthcoming Next Generation Network (NGN) is an all IP network. Multimedia communications over IP networks are a type of bundled session communications, which cannot directly traverse Network Address Translations (NATs) and firewalls even in NGN. To solve the problem that the existing traversal methods are not suitable for service providers to set up a real system in NGN,a Distributed Broker-agent Architecture (DBA) is addressed. DBA is secure and realizable for service providers and enterprises because it is easy to set up and does not need to upgrade the existing devices like Firewalls, NATs or endpoint devices of subscribers.DBA is composed of two-layer distributed agents, the server proxies and the client agents, in which all multimedia communications use shared tunnels to carry signaling messages and media data between broker-agents, and the call signaling is encrypted over Security Socket Layer (SSL) to guarantee the security of calling. Moreover, the function model and multiplexed connection messages format of DBA are designed, which lays a basis for the protocol in the future NGN. In addition, a simple implementation based on H.323 verifyies the main function of traversing firewalls and NATs.     

咪唑啉受体激动剂莫索尼定对家兔希氏束电位的影响

目的　观察咪唑啉受体激动剂 莫索尼定 (Mox)对家兔在体心脏希氏束电位的影响。方法　将 2 4只健康家兔随机分为 4组 :空白对照组 ;Mox小剂量组 (0 .1mg·kg-1) ;Mox中剂量组 (0 .3mg·kg-1) ;Mox大剂量组(0 .9mg·kg-1) ,采用经右侧颈总动脉插管法 ,记录希氏束电图 (HBE) ,同步测量体表心电图 (ECG)。结果　静脉注射Mox后 ,家兔ECG的R R间期、P R间期和HBE的H V间期较空白对照组明显延长 ,且呈剂量依赖性关系 ,给药前、后HBE的A H间期与空白对照组相比无明显差异。结论　静脉注射Mox能剂量依赖性延长家兔HBE的H V间期 ,而对A H间期无明显作用 ,提示Mox可延长激动在心脏传导系统室内部分的传导时间     

绞股蓝总皂甙对Lewis肺癌的作用

绞股蓝总皂甙（ＧｙｐｅｎｏｓｉｄｅｓＧＰ）对Ｌｅｗｉｓ肺癌原位肿瘤的生长及肺转移均有抑制作用。在１５、３０、６０ｍｇ／（ｋｇ·ｄ）的剂量下，原位肿瘤的抑制率分别为３１．８％、４５．２％、４９．３％，且能明显减少肺转移集落数，有明显剂量依赖关系。     

基于故障注入的CTCS-3级列控系统可靠性评估技术研究

CTCS-3级列车运行控制系统的可靠性是保证列车安全高效运行的必要条件,因此,在列车控制系统仿真系统上进行各种测试验证试验至关重要。故障注入技术（Fault Injection）是将系统的有效故障模式样本注入到系统中,以此评价系统的功能设计水平,是系统测试性、安全性以及功能验证研究的重要研究基础。根据CTCS-3级列车控制系统的运行特点和软件故障注入的优势,提出将软件故障注入方法应用在列控系统仿真领域进行可靠性验证,设计并实现了一种软件故障注入系统,给出其中的故障案例描述语言、故障库和故障注入软件算法,构建了软件故障注入系统的总体结构和各部分的详细功能结构。通过仿真结果可以看出,软件故障注入方法应用在CTCS-3级仿真系统中,可以有效地模拟故障并执行注入,进一步提高仿真系统的可靠性和容错性。     

浅谈山区弱风化带嵌岩桩基础开挖施工技术

结合贵州省新建织金至纳雍铁路Ⅱ标卡拉河大桥3#和4#主墩嵌固桩基础开挖的施工生产实践,对山区弱风化带矩形大体积基础小爆破碎裂开挖法及钢筋混凝土护壁施工工艺进行研究,在保证坑壁规则整齐、不震裂、不超挖、每循环护壁施工周期短的情况下,施工进度及效益均取得良好效果,在类似工程中具有一定的借鉴意义。     

跨既有铁路矮塔斜拉桥设计与转体施工

矮塔斜拉桥作为一种中等跨度桥梁形式,采用平面转体施工方法,用在上跨限界较宽的既有铁路线上,是一种合适的选择。以某墩顶平面转体法施工的2×70 m矮塔斜拉桥为例,阐述该桥型应用在上跨既有铁路线的优点,以及按平面转体方法施工的设计过程。     

高等数学教学方法的探讨

本文根据《高等数学》的课程特点,结合高职高专院校高等数学课程的教学现状,基于教学工作实践,提出在教学中注重理论联系实际,强调理论的应用,同时在高等数学知识传授中注意渗透数学的思想和方法来优化高等数学课程的教学,以达到现代教育培养综合素质高、应用能力强的复合型人才的目标。并且基于高等数学教学实践,通过实例,探讨了如何在教学中激发学生学习兴趣和培养学生的创新思维能力。     

含Josephson结电路的混沌形成过程

用数值模拟方法研究了含Josephson结电路的动力学行为.根据电路模型和基尔霍夫定律建立系统的动力学方程,求出系统的平衡点,利用系统的相平面图、分岔图和Lyapunov指数图分析系统的混沌形成过程.为在此系统中避免混沌提供了直接的线索,为Josephson结器件稳定工作提供了有价值的参考.     

Effects of pioglitazone on serum leptin and adiponectin in polycystic ovary syndrome patients with insulin resistance

Objective To investigate the effects of pioglitazone on serum leptin and adiponectin in polycystic ovary syndrome (PCOS) patients with insulin resistance (IR). Methods Thirty-five PCOS patients with IR were treated with pioglitazone 15mg/d for 12 weeks. The results of ovulation induction were observed. The changes of fasting plasma glucose (FPG), fasting serum insulin (FINS), serum levels of leptin, adiponectin, follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T) and blood fat were examined at the baseline and after the therapy by enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA). Results After the treatment, the rate of ovulation per cycle was improved in 31 (88.5%) out of the 35 patients. After treatment, the level of serum leptin was decreased (P<0.05) while the level of serum adiponectin was increased (P<0.05). After 12 weeks'treatment, waist-to-hip ratio and F-G score were significantly decreased (P<0.05), BMI declined but without significant difference (P>0.05). The levels of LH, T, FINS, Homa-IR, total cholesterol, triglyceride and low density lipoprotein-cholesterol were significantly decreased (P<0.01, P<0.05), whereas the level of high density lipoprotein-cholesterol was significantly increased (P<0.01). No significant difference was seen in FSH and FPG following treatment (P>0.05). Conclusion Pioglitazone treatment can effectively improve PCOS with IR patients'clinical syndromes, insulin sensitivity, glucose and lipid metabolism at least partly through improving the profiles of leptin and adiponectin.