人骨髓间充质干细胞生物学特性及腺病毒介导的GFP标记

目的建立人骨髓间充质干细胞(hMSCs)分离、培养以及鉴定的方法,同时应用腺病毒介导的绿色荧光蛋白(Ad-GFP)检测细胞转染的效率。方法应用人淋巴细胞分离液(Ficoll)对hMSCs进行分离,经贴壁纯化后用MTT法检测增殖能力;并对其成骨、成脂、成平滑肌诱导分别进行碱性磷酸酶(ALP)、油红O染色及α-SMA免疫细胞化学检测;采用流式细胞仪(FCM)检测细胞表面标志的表达。用Ad-GFP转染,并应用荧光显微镜与FCM检测转染效率。结果 hMSCs镜下为成纤维样形态,约在第4天进入对数增长期;分化诱导后ALP染色、Von kossa银染、油红O染色以及α-SMA均为阳性;FCM检测CD14、CD31、CD45表达阴性,CD44、CD73、CD106及PDGFRβ表达阳性;当感染复数MOI=200,细胞培养48h时感染效率较高,FCM检测显示为99.81%。结论经过对增殖、分化能力以及表面标志的分析,证明用Ficoll可以分离得到较纯的hMSCs,Ad-GFP可以很好地感染细胞,为组织工程种子细胞的体内示踪提供了途径。     

几种软基处理方式的比较

高速公路对路基沉降的要求很高,选择正确的地基处理方式直接关系到工程质量、投资和进度.总结了近年来软土地基处理技术及其发展的现状.     

大型循环流化床底部区域颗粒浓度波动研究

在内径400 mm、高9 100 mm的大型冷态循环流化床实验装置上利用光导纤维探头研究底部区域颗粒浓度的波动行为,并采用统计方法和功率谱分析了浓度波动.结果表明,正常流化条件下,颗粒浓度标准偏差在径向上随r/R的增大而增大,而随轴向位置的增高而减小;标准偏差随颗粒循环速率的增大而增大,而随表观气速的增大而减小.分析颗粒浓度波动的功率谱发现,主频不明显,0～10 Hz范围内振幅较大.实验结果为循环流化床装置的设计、数值模拟和工程放大提供了一定依据.     

Safety Evaluation for Freeway Exit Ramp Based on Speed Consistency

Speed differential has direct relationship with road safety. Conventional speed consistency measures draw sample data from independent population that follows a normal distribution. These methods may fall into ecological fallacy and overestimate the safety level of road elements. After deducing the relationship between the difference in operating speed ΔV₈₅ and85(ΔV), the 85^th percentile value of individual speed reduction, this paper recommends 85(ΔV) and speed reduction rate 85(ΔV)R is used to evaluate ramp safety. Point speeds of individual vehicle at freeway diverge area, upper ramp, and lower ramp are collected by radar guns. Safety performance of 37 exit ramps are appraised using three different measures, namely, ΔV₈₅, 85(ΔV), and 85(ΔV)R. The results show that 85(ΔV) is 1.42 to 2.02 times of ΔV₈₅, and that the evaluation result of 85(ΔV)R is between the other two. The measure 85(ΔV)R considers not only individual speed but also the base speed on upstream element, which avoid the pitfalls of ecological fallacy and overestimation possessed by conventional measures. It is a safer and reasonable measure that should be adopted in practice.     

Elastic Modulus Limit of Support Layer in Twin-Block Ballastless Track Based on Train Load Effect

The support layer is an important component of twin-block ballastless track. The modulus of the support layer is an important design parameter and must be carefully solved. We studied the bending stress and deformation of track slab and support layer due to train load using the beam-plate finite element model on elastic foundation. The results show that support layer type has great impact on both support layer deformation and the stress on subgrade, but has little impact on the bending stress of either track slab or support layer. The continuous support layer type, and articulated support layer type with shear transfer device at their ends, are recommended. In order to keep the stress in the support layer less than that in track slab, the modulus of the continuous, unit, and articulated types of support layer (in unit twin-block ballastless track), and the support layer in continuous twin-block ballastless track, should not be larger than 15, 22, 20.5 and 5 GPa, respectively. In addition, the modulus of the unit-type support layer should not be more than 20 GPa, to ensure the step in support layer remains less than 1 mm.     

浅谈沥青路面病害的处理

为保证道路的畅通、安全、舒适以减少养护费用,应对坑槽及时处理。对坑槽处理的材料和施工工艺进行了介绍,并分析了其寿命周期费用。     

钢筋锈蚀综合测量装置的研发

本文介绍的钢筋锈蚀综合测量装置是一种新型的、多参量、适合于现场测量的混凝土结构钢筋锈蚀状况的无损检测仪器。本仪器的研制,主要根据腐蚀电化学的原理,综合电化学的检测方法,应用先进的电子测量、测试技术和计算机技术,对钢筋混凝土结构的钢筋锈蚀的参量进行测量,从而,根据相关的评判标准对混凝土结构中的钢筋锈蚀状况进行评估,提供了科学的手段。本装置可广泛应用于公路交通、铁路、建筑、水利和其他相关领域的混凝土结构中钢筋状况的检测,为早期发现钢筋的锈蚀提供了先进的综合检测手段。     

雪峰山隧道平面控制测量技术

随着我国山区高速公路建设的迅猛发展,长大隧道的施工占据了相当的市场,以雪峰山隧道控制测量为例,介绍GPS卫星定位系统在长大隧道施工平面控制测量中的应用技术.     

乙醇/柴油发动机醇醛排放的测试技术研究

为了测量乙醇/柴油发动机新的排放物——乙醇和乙醛,对气相色谱分析测试技术进行了研究。通过对不同采样方法和采样条件的比较分析,确定了以去离子水作为吸收液的冰水浴两级吸收采样方法,实现了对发动机尾气中乙醇和乙醛的采样,吸收效率大于96%;通过引入采样系统稀释系数、流量校正系数和吸收效率修正系数的理论计算,提高了测量的准确度;建立了一套完整的色谱分析方法和色谱标定方法,利用气相色谱仪实现了对样品中乙醇和乙醛的分离定量测试;整个测量系统对排气中的乙醇和乙醛检测限值为0.1×10-6,最大极限误差为0.5×10-6,并在一台实际乙醇/柴油发动机上进行了应用。     

水下自主作业系统轨迹跟踪与动力定位

论述了一种水下自主作业系统的轨迹跟踪和动力定位控制算法.该系统由一个水下智能机器人和一个机械手组成,具有非线性、强耦合、高维数、时变等特点.在充分考虑了各种水动力因素的基础上,使用Quasi-Lagrange方程建立系统的动力学数学模型.采用滑模控制方法,并利用模糊逻辑动态调节滑膜控制器的增益系数,进行了计算机仿真试验.结果表明该方法对水下自主作业系统的轨迹跟踪与动力定位控制性能优良.