水飞蓟宾对人膀胱癌细胞株5637增殖的抑制作用及其机制

目的研究水飞蓟宾(Silibinin,SB)在体外对人膀胱癌细胞株5637增殖的抑制作用及其相关机制。方法采用MTT法观察不同浓度SB对人膀胱癌细胞株5637增殖的抑制作用;倒置显微镜下观察细胞形态变化;RT-PCR检测凋亡抑制因子Survivin的mRNA表达变化。结果MTT检测发现:随着SB浓度增加和作用时间延长,对人膀胱癌细胞株5637增殖的抑制作用增强,且各组间比较均有显著性差异(P<0.01),并呈时间-剂量依赖关系;倒置显微镜发现应用SB干预后,5637细胞体积缩小,变形,胞浆浓缩,核固缩深染;人膀胱癌细胞株5637中Survivin的mRNA表达随着SB浓度增加和时间延长相应下降。结论SB可抑制人膀胱癌细胞株5637的增殖;并下调凋亡抑制因子Survivin的mRNA表达。     

纳米化对1Cr17不锈钢耐高温氧化的影响

喷丸处理可以使铁素体不锈钢1Cr17实现表面自身纳米化.对不同工艺的试样进行在高温空气介质中的耐氧化试验,结果表明1Cr17不锈钢经纳米化喷丸后,在氧化20 h后材料的表面可以形成一层富Cr的致密的内氧化物层,有效的防止了材料的进一步氧化.喷丸后又经过退火的试样,在氧化12 h时表面就形成了一层致密的内氧化物层,因为退火可以有效地释放因为喷丸而产生的压应力,可以更好地促进Cr向表面的扩散,更快的在合金表面形成富Cr的氧化物,从而显著提高了合金的抗高温氧化性.     

角膜基质脱细胞处理与相容性实验研究

目的寻找有效适宜的脱细胞方法对猪角膜进行处理,制备角膜生物支架材料。方法利用胰酶和胶原酶消化结合液氮冻融的方法对猪角膜基质进行处理,60Co消毒,冻干保存。经组织学观察、细胞培养附和与动物移植检验其生物特性。结果获得的脱细胞角膜基质,显微结构观察显示板层结构完整,胶原纤维间组织结构疏松,未见细胞残留,整个基质成网状结构,在上皮面保存有完整连续的基底膜。细胞培养附和实验观察到种子细胞可以在基质上良好生长。动物移植实验未见明显的炎症反应与排斥。结论经该法脱细胞处理后得到的角膜基质无细胞毒性,组织相容性好,可以成为适宜的组织工程支架材料。     

脾虚证大鼠侧脑室注射孤啡肽对细胞免疫功能的影响

目的脾虚证大鼠侧脑室注射孤啡肽,研究其对细胞免疫功能的影响,从神经内分泌的角度探索脾虚的细胞免疫学变化本质。方法用100%大黄煎剂灌胃复制脾虚证大鼠模型,四君子汤作为反证药物,侧脑室埋管,注射孤啡肽,MTT法检测脾T淋巴细胞增殖能力、足跖增厚法检测迟发型变态反应程度,判定细胞免疫功能改变情况。结果灌服大黄煎剂后,大鼠逐渐出现腹泻,或伴有脱肛,食量减少,精神不振,懒动,被毛失去光泽,形体消瘦等脾虚症状,而空白对照组(BC组)没有上述症状;T淋巴细胞增殖能力、迟发型变态反应程度与BC组比较明显减弱(P<0.01)。四君子汤治疗组(DFMD组)和孤啡肽组(OFQ组)大鼠上述脾虚症状及检测指标明显改善(P<0.01)。结论脾虚证大鼠侧脑室注射孤啡肽能提高细胞免疫功能。     

生物接触氧化法处理洗浴废水的填料选择研究

生物接触氧化法处理洗浴废水的填料选择研究以软性纤维和软性泡沫作为备选填料,采用活性污泥挂膜法,浅析了膜培养过程中的影响因素.最后通过最佳水力停留时间这一废水处理指标对备选填料进行比较,最终选择软性泡沫作为动态实验填料.     

面向驾驶员的城市出租汽车服务站调查——以广西壮族自治区柳州市为例

城市出租汽车服务站普遍存在建设无序与低效的情况,需进行合理布局规划。以广西壮族自治区柳州市为例,对出租汽车驾驶员进行问卷调查。分析出租汽车驾驶员特征、驾驶员对服务站功能的选择意向,并采用分形理论分析服务站选址分布与城市空间形态的关系。结果显示:出租汽车服务站的服务功能应以解决驾驶员停车、休息、就餐、如厕等基本需求为主;驾驶员年龄因素对服务功能选择有较为明显的影响;服务站的规划建设应密切依托城市空间形态及城市道路网、人口、用地等城市构成要素分布。通过将出租汽车微观数据与城市宏观形态相结合,为城市出租汽车服务站规划与评价提供新的视角。     

Product Quality Evaluation Method Based on Product Gene Theory

Traditional quality inspection based product quality evaluation method with complex process has high operating cost and requires more professional knowledge. To remove the above limitation, this paper leads product gene theory into product quality evaluation. Methods of quality influencing factors based modeling and encoding are established. Combined with similarity theory and product gene theory, a product gene similarity analysis based quality evaluation method is proposed. The proposed method is low cost, operates easily and requires less specialized knowledge. A case study is conducted to prove the correctness and feasibility of this method.     

Structural Optimization of Hatch Cover Based on Bi-directional Evolutionary Structure Optimization and Surrogate Model Method

Weight reduction has attracted much attention among ship designers and ship owners. In the present work, based on an improved bi-directional evolutionary structural optimization (BESO) method and surrogate model method, we propose a hybrid optimization method for the structural design optimization of beam-plate structures, which covers three optimization levels: dimension optimization, topology optimization and section optimization. The objective of the proposed optimization method is to minimize the weight of design object under a group of constraints. The kernel optimization procedure (KOP) uses BESO to obtain the optimal topology from a ground structure. To deal with beam-plate structures, the traditional BESO method is improved by using cubic box as the unit cell instead of solid unit to construct periodic lattice structure. In the first optimization level, a series of ground structures are generated based on different dimensional parameter combinations, the KOP is performed to all the ground structures, the response surface model of optimal objective values and dimension parameters is created, and then the optimal dimension parameters can be obtained. In the second optimization level, the optimal topology is obtained by using the KOP according to the optimal dimension parameters. In the third optimization level, response surface method (RSM) is used to determine the section parameters. The proposed method is applied to a hatch cover structure design. The locations and shapes of all the structural members are determined from an oversized ground structure. The results show that the proposed method leads to a greater weight saving, compared with the original design and genetic algorithm (GA) based optimization results.     

Catalytic Combustion of Lean Methane Assisted by Electric Field over Pd/Co<Subscript>3</Subscript>O<Subscript>4</Subscript> Catalysts at Low Temperature

A series of Pd/Co₃O₄ catalysts were prepared by Self-Propagating High-Temperature Synthesis (SHS) method in this study, and electric field was applied for catalytic combustion of lean methane over Pd/Co₃O₄ catalysts at low temperature. When electric field was applied, the catalytic combustion performance of Pd/Co₃O₄ catalysts was greatly improved, and the application of electric field could reduce the load of active element Pd to some extent while maintaining the same efficiency. Based on experimental tests and the analysis results of X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), H₂-temperature-programmed reduction (H₂-TPR) and in-situ diffuse reflectance infrared Fourier transform spectroscopy (in-situ DRIFTS), the mechanism of catalytic oxidation of CH₄ over Pd/Co₃O₄ catalysts in electric field was proposed. The catalytic combustion of CH₄ occurs only when the temperature is higher than 250 °C normally, but when electric field was applied, the whole process of CH₄ oxidation was promoted significantly and the reaction temperature was reduced. Electric field could promote the reduction of the support Co₃O₄ to release the lattice oxygen, resulting in the increase of PdO_x and the surface chemisorbed oxygen, which could provide more active sites for the low-temperature oxidation of CH₄. Furthermore, electric field could accelerate the dehydroxylation of CoOOH to further enhance the activity of the catalysts.     

Geometric calibration algorithm of polarization camera using planar patterns

Polarization pattern provides additional information besides spectral signatures. It can be used in many applications, such as navigation, defect detection and object identification. A novel polarization camera composed of four synchronized cameras is proposed, and it can realize real-time polarization measurement. This study particularly concentrates on the geometric calibration of the system. The projection model is analyzed and the multi-camera calibration algorithm is proposed. Firstly, each camera is calibrated separately using planar patterns, and then the geometric calibration algorithms are performed. Due to the geometrical constraint, a global optimization method results in smaller estimation uncertainties. A mean rotation error of 0.025° and a mean translation error of 0.26mm are achieved after geometric calibration. The images are rectified to establish a correspondence among cameras and are combined to acquire the polarization measurement. The polarization pattern of the skylight is measured by the system and the results are consistent with the previous studies.