外源磷脂酰乙醇胺诱导人宫颈癌HeLa细胞凋亡的研究

目的 磷脂酰乙醇胺(phosphatidylethanolamine, PE)在细胞凋亡的早期由细胞膜内侧翻转到外侧,与磷脂酰丝氨酸共同成为细胞凋亡的早期信号,但是其在凋亡中的作用尚不清楚.本研究探讨了PE对HeLa细胞凋亡的影响.方法 实验以人宫颈癌HeLa细胞系为材料,分别设置了空白对照组、0.125、0.25、0.5和1 mmol/L PE处理组.以MTT检测细胞生长情况,PI-流式细胞仪法检测细胞周期,Annexin V-PI法检测细胞凋亡.结果 与对照组相比,PE各处理组对HeLa细胞生长的抑制作用呈剂量与时间依赖性,并诱导细胞凋亡发生,但不影响细胞周期.结论 PE通过诱导细胞凋亡而抑制HeLa细胞的生长.     

Union examination of AFP, AFU, AFPL3 and γ-GT in early diagnosis of primary liver cancer

Objective To explore the significance of union examination of blood serum liver cancer tracers in the early diagnosis of liver cancer. Methods We observed and compared the level of blood serum liver cancer tracers armor embryo protein (AFP), crag algae glycosidase (AFU), armor embryo protein heteroplasmon (AFPL3) andγ-Gu Anxian transferase (γ-GT) in early time for primary liver cancer patients and hepatitis liver cirrhosis patients and those chronic hepatitis B patients who had liver cancer family history. Results Finally among the 30 patients in the early liver cancer group, 23 were positive with AFP, 20 with AFU, 15 with AFPL3 and 21 with γ-GT. Five were found positive with blood serum AFP, AFPL3, AFU and γ-GT at the same time; 5 with AFP, AFPL3 and γ-GT; 5 with AFP, AFU and AFPL3; 7 with AFP, AFU andγ-GT. By contrast, in the control group, among the 30 hepatitis liver cirrhosis patients and those chronic hepatitis B patients with liver cancer family history, 11 were found positive with AFP, 3 with AFPL3, 12 with AFU and 14 with γ-GT. None of the patients were found positive with union examination of AFP, AFPL3, AFU and γ-GT in the blood serum at the same time. Conclusion The union examination of AFP, AFU, AFPL3 and γ-GT is significant to the early diagnosis of primary liver cancer.     

Screening of hepatocyte proteins binding to NS5ABP37 protein by yeast-two hybrid system

Objective To investigate the biological function of NS5ABP37 and to look for proteins interacting with NS5ABP37 protein in hepatocytes. Methods We constructed bait plasmid expressing NS5ABP37 protein of hepatitis C virus (HCV) by cloning the gene of NS5ABP37 protein into pGBKT7, then the recombinant plasmid DNA was transformed into yeast AH109 (α type). The transformed yeast AH109 was mated with yeast Y187 (α type) containing liver cDNA library plasmid in 2×YPDA medium. Diploid yeast was plated on synthetic dropout nutrient medium (SD/-Trp-Leu-His-Ade) containing X-α-gal for selection and screening. After extracting and sequencing of plasmids from positive (blue) colonies, we made a sequence analysis by bioinformatics. Results We screened twenty-five proteins binding to NS5ABP37, including Homo sapiens cyclin Ⅰ (CCNI) gene, Homo sapiens matrix metallopeptidase 25 (MMP25) and Homo sapiens talin 1. Conclusion The yeast-two hybrid system is an effective method for identifying hepatocyte proteins interacting with NS5ABP37 of HCV. And the biological function of NS5ABP37 may be associated with glycometabolism, lipid metabolism and apoptosis.     

NS1602型铁路起重机架设六四式铁路军用梁技术研究

介绍了利用NS1602型铁路起重机架设32m六四式铁路军用梁的主要过程,并对架梁过程中已架钢梁的安全问题进行了验算：确定不利工况下的起重机支腿反力,检算反力作用下钢梁的强度及稳定性。计算表明,利用铁路起重机架设抢修钢梁技术可行、安全可靠,为应急抢修架设军用梁提供了一个新的途径。     

收缩徐变对混凝土斜拉桥成桥状态的影响分析

运用最小弯曲能量法和应力平衡法相结合的方法来确定混凝土斜拉桥的合理成桥状态,就不同成桥状态下收缩徐变对主梁弯矩的影响程度进行了分析,并对其原因进行了探讨。总结了收缩徐变对结构受力影响的普遍规律,收缩徐变在斜拉桥桥塔附近梁段将产生负弯矩、在跨中附近梁段将产生正弯矩。得出了主梁中预应力的配置以及弯矩的调整情况都会对收缩徐变改变主梁弯矩的程度造成影响这一结论。并对不同成桥状态下收缩徐变趋于稳定后的主梁弯矩进行了比较,得出不同成桥状态下收缩徐变趋于稳定后主梁弯矩比较接近这一规律。     

土石混填路堤压实质量检测及控制方法分析

通过对路基传统检测方法的评价,分析其在土石路堤检测中的不足,提出了静力贯入法与面波检测法来控制土石路堤的压实质量,是一种有发展前景的检测方法.     

沥青组分对沥青与集料粘附性影响的研究

用沥青的四组分法分离沥青为几个化学组分,研究了各部分的性质;探索了在不同温度下各组分之间的转化规律,研究了沥青各组分对沥青与集料粘附性的影响,为提高沥青混凝土的抗水损坏性能提供了思路.     

色彩在沥青类路面铺装中的运用

色彩是彩色铺装的灵魂。简要介绍色彩的基本属性和特性,分析色彩的意向和用途,阐述、归纳现阶段一些沥青类彩色路面的主流着色方案和铺装方案,以期对广大彩色路面设计研究人员提供帮助。     

Development of an intelligent CAE system for auto-body concept design

The concept design phase is a critical step in auto-body design, as it has a great effect on later design work. This paper describes the implementation of an auto-body structure design in the early stages of a new auto-body developing program. In order to reduce the long design period and analysis error that plagues traditional auto-body concept design, an intelligent CAE system has been successfully developed and implemented based on the UGS NX/API opening platform. This system, the so-called ACD-ICAE (Auto-body concept design-intelligent computer aided engineering) System that means concept design-intelligent computer aided engineering system, employs a fully parametrized template method to build the conceptual auto-body geometry model and FEM model quickly and easily. It also integrates auto-body modeling, analysis and optimization on only one CAD platform via a parametric variables database. Moreover, all parametric variables are shared and updated in different phases of the ACD-ICAE System. A wizard User Interface (UI) based on knowledge of auto-body engineering was developed and used in this system. The procedures implementing the functional diagram of the ACD-ICAE system are also provided. A typical example of a car body concept design with four doors shows that the ACD-ICAE system is efficient and accurate.     

特长公路隧道通风竖井施工方法分析

目前随着山岭重丘区高速公路建设的日益增多,对环境保护方面的工作也日益重视。高速公路特长隧道的设计逐步增多,通风竖井作为特长隧道的通风设施普遍采用。结合广梧高速公路石牙山隧道通风竖井的施工工艺进行分析。