导电密封胶对不锈钢车体电阻点焊焊接性能影响研究

分析了电阻点焊原理,对相同的点焊搭接方式,分别采用3种工况进行焊接试验。3种工况分别采用相同焊接参数对搭接接触面涂导电密封胶和不涂导电密封胶的试件进行焊接;优化焊接参数后,对搭接接触面涂导电密封胶的试验件进行焊接。对3种工况下完成的焊接试件进行拉伸力检测试验、金相熔核尺寸检测和缺陷检测试验,对熔核直径、拉伸力、熔核内部缺陷的试验结果进行分析。试验结果表明:导电密封胶对电阻点焊的焊接性能存在影响;通过适当优化焊接参数,可以提高涂导电密封胶试件的焊接性能,焊接质量能够满足产品焊接要求。     

电控转向系统直流电机实时故障诊断方法

作为电动助力转向系统和线控转向系统执行元件的电动机,其可靠运行对电控转向汽车的行驶安全至关重要.本文中通过建立永磁直流电机模型和运用带有遗忘因子的递推最小二乘法,提出了一种电控转向系统直流电机故障诊断方法.线控转向实车试验验证的结果表明,该方法可准确、实时地诊断直流电机故障,为电控转向系统的容错控制奠定基础.     

港口供电系统改进

目前港口供电系统存在体积大、结构设置不合理和不适应环境等问题,经研究提出了减小体积、优化系统配置、提高箱变安全性和环境适应性等相应对策,在研究的基础上设计了一种新型箱式变电站,解决了目前港口供电系统存在的不足.     

Subjective-utility travel time budget modeling in the stochastic traffic network assignment

ABSTRACT

In this article, we propose a new model called subjective-utility travel time budget (SU-TTB) model to capture travelers' risk-averse route choices. In the travel time budget (TTB) and mean-excess travel time (METT) model, a predefined confidence level is needed to capture the risk-aversion in route choice. Due to the day-to-day route travel time variations, the exact confidence level is hard to be predicted. With the SU-TTB model, we assume travelers' confidence level belongs to an interval that they may comply with in the route choice. The two main components of SU-TTB are the utility function and the TTB model. We can show that the SU-TTB can be reduced to the TTB and METT model with proper utility function for the confidence levels. We can also prove its equivalence with our recently proposed nonlinear-expectation route travel time (NERTT) model in some cases and give some new interpretation on the NERTT with this equivalence. Finally, we formulate the SU-TTB model as a variational inequality (VI) problem to model the risk-averse user equilibrium (RAUE), termed as generalized RAUE (GRAUE). The GRAUE is solved via a heuristic gradient projection algorithm, and the model and solution algorithm are demonstrated with the Braess's traffic network and the Nguyen and Dupuis's traffic network.     

State estimation in roll dynamics for commercial vehicles

Accurate lateral load transfer estimation plays an important role in improving the performance of the active rollover prevention system equipped in commercial vehicles. This estimation depends on the accurate prediction of roll angles for both the sprung and the unsprung subsystems. This paper proposes a novel computational method for roll-angle estimation in commercial vehicles employing sensors which are already used in a vehicle dynamic control system without additional expensive measurement units. The estimation strategy integrates two blocks. The first block contains a sliding-mode observer which is responsible for calculating the lateral tyre forces, while in the second block, the Kalman filter estimates the roll angles of the sprung mass and those of axles in the truck. The validation is conducted through MATLAB/TruckSim co-simulation. Based on the comparison between the estimated results and the simulation results from TruckSim, it can be concluded that the proposed estimation method has a promising tracking performance with low computational cost and high convergence speed. This approach enables a low-cost solution for the rollover prevention in commercial vehicles.     

幽门螺杆菌尿素通道蛋白基因UreI载体的构建、融合表达及纯化

目的构建幽门螺杆菌(H.pylori,Hp)尿素通道蛋白基因UreI的融合表达载体,并在E.coliBL21中表达,为进一步研究UreI的功能奠定基础。方法利用分子克隆技术以Hp DNA染色体为模板,扩增Hp UreI基因片段,将目的基因UreI与载体pET32a( )分别经kpnⅠ和HindⅢ双酶切,纯化,连接。筛选阳性重组载体,转化大肠杆菌BL21,以IPTG诱导表达pET32a( )/UreI融合蛋白。表达产物经Ni-NTA琼脂糖树脂纯化后,用SDS-PAGE及Western blot分析。结果经酶切、测序分析表明,插入的基因片段为Hp UreI编码基因,由585 bp组成,与GenBank相应菌株HP AM417 609序列完全一致,无碱基突变。经SDS-PAGE分析显示重组质粒pET32a( )/UreI在原核细胞中得到了高效融合表达,其重组融合蛋白分子量为130 ku,经Ni-NTA琼脂糖树脂纯化后,其纯度达95%以上,Western blot分析显示,该重组融合蛋白可被6-His鼠抗单克隆抗体所识别,具有良好的反应原性。结论成功地克隆和构建了原核表达载体pET32a( )/UreI,并在原核细胞中得到了高效融合表达。     

掺锶磷酸钙骨水泥的体外细胞生物学性能评价及其与掺锶量的相关性

目的 评价"SrHPO4/Ca4(PO4)2O/CaHPO4"系掺锶磷灰石骨水泥的生物相容性及其与掺锶量的关系.方法 通过急性全身毒性、热原性、溶血性、体外细胞毒性以及成骨细胞在材料表面的黏附、增殖与表达等实验,检测材料的性能及生物降解潜能.结果 "SrHPO4/Ca4(PO4)2O/CaHPO4"系掺锶磷灰石骨水泥的急性全身毒性、热原性、溶血性、体外细胞毒性均为合格.Sr-CPC的热原性、溶血性以及碱性磷酸酶值与掺锶量呈非线性关系.结论 含锶磷酸钙骨水泥体外细胞生物相容性良好,是安全的新型骨组织工程支架材料.     

快速老化小鼠的学习记忆缺陷及其生化机制

目的用快速老化小鼠(SAMP8)模拟老年痴呆,观察其学习记忆能力,并探讨其学习记忆能力下降的可能机制。方法采用10月龄SAMP8,并用同龄抗快速老化小鼠(SAMR1)作为正常对照。用水迷路检测近记忆以及空间学习记忆能力。用生物化学方法检测大脑皮层线粒体膜电位和ATP酶活力,观察线粒体功能;检测皮层和海马胆碱乙酰基转移酶(ChAT)和乙酰胆碱酯酶(AChE)的活力,观察中枢胆碱能神经功能;检测血清超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量,观察其全身性氧化应激状况。结果与SAMR1相比,SAMP8出现明显的衰老体征,其近记忆和空间学习记忆能力明显减弱,具有衰老和痴呆特征;皮层线粒体膜电位和ATP酶活力显著降低,海马ChAT活力显著下降,AChE活力显著升高,血清SOD活力稍有增高。结论SAMP8可以很好地模拟老年痴呆,其机制包括大脑皮层线粒体功能降低、海马胆碱能神经功能下降以及氧化应激等。     

行政法与行政诉讼法教学法探析

“行政法与行政诉讼法学”是一门复杂的有较强理论性但又必须和社会实际相结合的课程。要提高该课程的教学质量,改善教学效果,文中提出了案例教学法、多媒体技术与模拟法庭教学法。     

地震作用下钢筋混凝土桥墩抗倒塌性能研究

为研究混凝土桥墩的结构抗震性能,进行了单墩多自由度体系非线性静力推倒分析、非线性动力分析、增量动力分析。分别采用纤维模型中分布塑性铰和集中塑性铰两种建模方法,建立单墩有限元模型,利用Open Sees软件中的Hysteretic滞回模型考虑各种累积损伤的影响。