昆明市“月月无车日活动”及公众调查分析

调查了解公众对无车日活动常态化这一公共政策的反应,能够为城市交通政策的制定提供一定的技术参考。介绍了昆明市城市交通概况及首个无车日活动成效,同时,对“月月无车日活动”做了概述,在总结前几次活动经验的基础上,给出了活动的进一步调整方案。通过对无车日活动公众调查问卷的数据分析,得出大多数公众对“月月无车日活动”的态度是理解和支持的;在无车日当天,私人小汽车拥有者选择公共交通作为替代小汽车出行方式的比例最高。另外,大力发展公共交通也被受访者认为是解决城市交通拥堵应当首选的措施。     

行人微观仿真中的运动模型与行为模型——STEPS与NOMAD模型对比

为了解各类行人仿真模型的性能差异和适用范围,以选取适当模型进行交通枢纽行人仿真,对两种典型行人仿真模型进行研究。依据模型对行人行为描述的复杂程度,将行人仿真模型分为运动模型、部分行为模型、行为模型3类,并分别介绍了各自的特点。选取典型的运动模型软件STEPS和行为模型软件NOMAD,详细探讨其采用的数学模型,并通过4个仿真实例对比分析这两种模型的特点。分析结果得出两种模型在宏观与微观层面的异同点以及存在的问题。     

纤维与沥青胶结料的抗拉拔性能评价

通过纤维拉拔试验来评价纤维与沥青胶结料的界面粘结强度,提出了拉拔过程中纤维与沥青胶结料相互作用的受力模型;利用此试验对聚酯纤维和芳纶纤维进行了试验研究,分析了影响界面粘结强度的主要因素,给出了沥青混合料中纤维的合理长度。     

Identity-based broadcast encryption with shorter transmissions

This paper describes two identity-based broadcast encryption(IBBE) schemes for mobile ad hoc networks.The first scheme proposed achieves sub-linear size cipertexts and the second scheme achieves O(1)-size ciphertexts.Furthermore, when the public keys are transmitted, the two schemes have short transmissions and achieve O(1) user storage cost, which are important for a mobile ad hoc network.Finally, the proposed schemes are provable security under the decision generalized bilinear Di?-Hellman(GBDH) assumption in the random oracles model.     

Power network parameter estimation method based on data mining technology

The parameter values which actually change with the circumstances, weather and load level etc.produce great effect to the result of state estimation. A new parameter estimation method based on data mining technology was proposed. The clustering method was used to classify the historical data in supervisory control and data acquisition (SCADA) database as several types. The data processing technology was impliedto treat the isolated point, missing data and yawp data in samples for classified groups. The measurement data which belong to each classification were introduced to the linear regression equation in order to gain the regression coefficient and actual parameters by the least square method. A practical system demonstrates the high correctness, reliability and strong practicability of the proposed method.     

刀豆蛋白A条件化培养基对体外培养的兔角膜内皮细胞生长的影响

目的研究刀豆蛋白A(concanavalin A,ConA)条件化培养基对体外培养的兔角膜内皮细胞(rabbit corneal endothelial cells,RCECs)生长的影响。方法采用揭取后弹力层及内皮细胞联合组织块法进行RCECs的体外原代培养,分别用含有体积分数为5%、10%、15%、20%ConA的活化大鼠脾细胞条件上清液的RPMI1640培养基进行RCECs原代培养,并用含10%(体积分数)胎牛血清(fetal bovine serum,FBS)的RPMI1640培养基作为对照。神经烯醇化酶免疫组织化学染色法进行细胞鉴定;噻唑蓝比色法观察各组RCECs生长的状况;SPSS11.0One-way ANOVA及LSD检验进行统计分析。结果成功进行了RCECs的体外培养,培养细胞内神经烯醇化酶阳性表达。ConA条件化培养基各组细胞增殖均高于对照组(P<0.001),且10%、15%ConA条件化培养基组的RCECs细胞数量明显高于其他各实验组(P<0.001)。结论ConA条件化培养基具有促进RCECs生长的作用,可作为培养辅佐剂。     

QoS awared power and admission controls based on non-cooperative game theory in wireless networks

In order to better accommodate heterogeneous quality of service (QoS) in wireless networks, an algorithm called QeS-aware power and admission controls (QAPAC) is proposed. The system is modeled as u non- cooperative game where the users adjust their transmit powers to maximize the utility, thus restraining the interferences. By using adaptive utility functions and tunable pricing parameters according to QoS levels, this algorithm can well meet different QoS reqniremcnts and improve system capacity compared with those that ignore the QoS differ- ences.     

Role of candesartan against cerebral ischemia and oxidative damage in normotensive rats

Objective Angiotensin Ⅱ (Ang Ⅱ ) contributes to modulating blood pressure by stimulation of Ang Ⅱ AT1 receptors. We devised a rat transient middle cerebral artery occlusion (MCAO) model to assess whether oxidative damage is decreased after pretreatment with Angiotensin Ⅱ AT1 receptor blocker (ARB). Methods After 2 weeks pretreatment with ARB 0. 5 and 1 mg/kg, the male Wister rats were subjected to 2 h middle cerebral artery occlusion (MCAO). At 24 h, the lumen diameter of middle cerebral artery, the plasma level of 8-hydroxy-2'-deoxyguanosine (8-OHdG), and HIF-1 a levels were recorded and compared. Results After pretrcatment with ARB 0.5 and 1 mg/kg, blood pressure did not significantly change compared with that of controls. In the group of candesartan at 1 mg/(kg· day), the lumen diameter was significantly increased compared to that in control group [(86.0±5.0) μm vs. (69.0± 2.1) μm; P<0. 01, n = 6- 8]. The plasma 8-OHdG levels of ARB pretreatment groups were decreased. In immunohistochemical findings, 8-OHdG- and HIF-1α-containing cells in ARB pretreatment groups were decreased. Conclusion Brain ischemia and oxidative damage can be reversed by AT1 receptor blockade in normotensive rats after transient cerebral artery occlusion.     

The role of interleukin-8 produced by tumor induced fibroblasts in the development of cutaneous melanoma

Objective To determine the role of interleukin-8 (IL-8) produced by tumor induced fibroblasts in the development of cutaneous melanoma. Methods B16 melanoma cells induced L929 fibroblasts phenotype was transdifferentiated to myofibroblasts (MF) by co-culture in vitro. MF was monitored by morphology and immunophenotype for a-SMA. The level of IL-8 was detected by ELISA. The effect on B16 cell proliferation rate was estimated using MIT method in vitro. Melanoma implanting model was constructed in C57 mice. Results L929 MF phenotype could be modulated by B16 melanoma cells-derived transforming growth factor-β1 (TGF-β1) and elevated the levels of IL-8. L929 MF did not influence the B16 melanoma cells viability in vitro, but shortened the time of tumor formation and increased the incidence rates of tumors in C57 implanting model mice. Conclusion Fibroblasts can be activated by tumor cells and produce IL-8, which acts as an inflammatory cytokine promoting the development of cutaneous melanoma.     

The changes of neuronal activity of the zone incerta in a rodent model of Parkinson＇s disease

Objective To study the changes in neuronal activity of the zone incerta (ZI) following the unilateral lesion of the nigrostriatal pathway. Methods Eiectrophysiological recordings of ZI neurons were made in normal rats and in two groups of rats at different time intervals after injection of 6-hydrodopamine (6-OHDA) into the pars compacta of snbstantia nigra by extracellular recording in vivo. Results The results showed a significant increase in the mean firing rate of ZI during the second and fourth weeks after 6-OHDA lesion [-n=32, (3.6±2. 2)Hz, P<0.001; n=35, (9.3±6.6)Hz, P<0. 001, respectively] compared to that of normal rats [n=39, (9.2±5.2)Hz]. However, no significant change was observed between two groups of 6-OHDA-lesioned rats. With regard to firing pattern, 7.7%(3/39) of ZI neurons discharged regularly, 82.1% (32/39) irregularly and 10. 3% (4/39) in bursts in normal rats. During the second week after 6-OHDA lesion, the regular, irregular and bursting firing neurons recorded in the ZI were 9.4% (3/32), 59. 4% (19/32) and 31.3% (10/32), and during fourth week, the regular, irregular and bursting firing neurons were 14.3% (5/35), 57.1% (20/35) and 28. 6% (10/35), respectively. The firing pattern of the neurons in the three different groups did not change significantly. Conclusion These results suggest that the firing rate of ZI neurons in 6-OHDA-lesioned rats is increased significantly, which may contribute to the pathophysiology of Parkinson's disease.